A 3D-impedimetric immunosensor based on foam Ni for detection of sulfate-reducing bacteria

A 3D-immunosensor based on simple and efficient trapping platform (foam Ni) combining with adsorption of gold nanoparticles and specific recognition of biological/chemical molecular has been reported for detection of sulfate-reducing bacteria (SRB) using electrochemical impedance spectroscopy (EIS). The impedance spectra were also used to characterize the successful construct and stepwise modification of the impedimetric immunosensors. This results show that a linear relationship between electron-transfer resistance (R(ct)) values and the logarithm of the SRB concentrations was obtained for the SRB concentration range of 2.1 x 10(1)-2.1 x 10(7) cfu/ml. Additionally, the fabricated immunosensor shows a high selectivity against other bacteria. (C) 2010 Elsevier B.V. All rights reserved.

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