A spectrophotometric assay for gentamicin.

A rapid and accurate spectrophotometric assay has been developed for the determination of blood werum levels of gentamicin and related antibiotics. The assay uses a purified enzyme from Escherichia coli JR88/C600 that acetylates gentamicin with the production of coenzyme A, linked to a chemical reaction with a sulfhydryl reagent to produce stoichiometric amounts of a sensitive chromophore, monitored in the visible spectrum. The system provides advantages of speed, cost, convenience, accuracy, and enzyme stability to the desirable characteristics encountered with previous enzymatic methods.

[1]  J. Waitz,et al.  Comparison of Enzymatic and Microbiological Gentamicin Assays , 1974, Antimicrobial Agents and Chemotherapy.

[2]  R. Holmes,et al.  Enzymatic assay for gentamicin and related aminoglycoside antibiotics. , 1974, The Journal of infectious diseases.

[3]  D. Smith,et al.  Gentamicin:adenine mononucleotide transferase: partial purification, characterization, and use in the clinical quantitation of gentamicin. , 1974, The Journal of infectious diseases.

[4]  M. Haas,et al.  Enzymatic Acetylation as a Means of Determining Serum Aminoglycoside Concentrations , 1973, Antimicrobial Agents and Chemotherapy.

[5]  J. Weinstein,et al.  Gentamicin resistance in strains of Pseudomonas aeruginosa mediated by enzymatic N-acetylation of the deoxystreptamine moiety. , 1972, Biochemistry.

[6]  R. Benveniste,et al.  R‐factor mediated gentamicin resistance: A new enzyme which modifies aminoglycoside antibiotics , 1971, FEBS letters.

[7]  J. Davies,et al.  Enzymatic acetylation of aminoglycoside antibiotics by Escherichia coli carrying an R factor. , 1971, Biochemistry.

[8]  J. Wagner,et al.  Gentamicin dosages for renal insufficiency. Adjustments based on endogenous creatinine clearance and serum creatinine concentration. , 1971, Annals of internal medicine.

[9]  F. G. Falco,et al.  Clinical research experience with gentamicin. Incidence of adverse reactions. , 1970, The Medical journal of Australia.

[10]  J F Murray,et al.  Determination of sulfhydryl groups with 2,2'- or 4,4'-dithiodipyridine. , 1967, Archives of biochemistry and biophysics.

[11]  S. Appel,et al.  A SPECTROPHOTOMETRIC ASSAY FOR THIOGALACTOSIDE TRANSACETYLASE. , 1965, The Journal of biological chemistry.

[12]  G. Ellman,et al.  Tissue sulfhydryl groups. , 1959, Archives of biochemistry and biophysics.