Detection of proliferating hepatocytes by immunohistochemical staining for proliferating cell nuclear antigen (PCNA) in patients with acute hepatic failure.

This study evaluated whether liver regeneration could take place after massive or submassive necrosis of liver cells in 25 patients with several kinds of hepatic failure by immunohistochemical staining for proliferating cell nuclear antigen (PCNA). PCNA positivity was significantly higher (P less than 0.01) in the patients who survived than in the patients who died. Furthermore, PCNA-positive hepatocytes were recognized diffusely in the lobule of the liver in survivors. There was positive correlation between PCNA positivity and plasma concentration of AFP (alpha-fetoprotein), (r = 0.77, P less than 0.01). These results show that liver regeneration could take place after massive necrosis of liver cells in survivors from acute hepatic failure and that immunohistochemical staining for PCNA is useful for prognostic evaluation.

[1]  Y. Hongo,et al.  [Immunohistochemical stain using anti-PCNA antibody in pancreatic tumors]. , 1991, Nihon Shokakibyo Gakkai zasshi = The Japanese journal of gastro-enterology.

[2]  P. Galand,et al.  Cyclin/PCNA immunostaining as an alternative to tritiated thymidine pulse labelling for marking S phase cells in paraffin sections from animal and human tissues , 1989, Cell and tissue kinetics.

[3]  Rochelle L. Garcia,et al.  Analysis of proliferative grade using anti-PCNA/cyclin monoclonal antibodies in fixed, embedded tissues. Comparison with flow cytometric analysis. , 1989, The American journal of pathology.

[4]  B. Calabretta,et al.  Inhibition of cellular proliferation by antisense oligodeoxynucleotides to PCNA cyclin. , 1988, Science.

[5]  R. Bravo,et al.  Cyclin/PCNA is the auxiliary protein of DNA polymerase-δ , 1987, Nature.

[6]  B. Stillman,et al.  Functional identity of proliferating cell nuclear antigen and a DNA polymerase-δ auxiliary protein , 1987, Nature.

[7]  J. Celis,et al.  Cell cycle-dependent variations in the distribution of the nuclear protein cyclin proliferating cell nuclear antigen in cultured cells: subdivision of S phase. , 1985, Proceedings of the National Academy of Sciences of the United States of America.

[8]  R. Bravo,et al.  Changes in the nuclear distribution of cyclin (PCNA) but not its synthesis depend on DNA replication. , 1985, The EMBO journal.

[9]  B. Franza,et al.  Identity of the proliferating cell nuclear antigen and cyclin , 1984, Nature.

[10]  Y. Takasaki,et al.  A nuclear antigen associated with cell proliferation and blast transformation , 1981, The Journal of experimental medicine.

[11]  E. Tan,et al.  Autoantibody to a nuclear antigen in proliferating cells. , 1978, Journal of immunology.

[12]  T. Waldmann,et al.  alpha-fetoprotein in noneoplastic hepatic disorders. , 1975, JAMA.

[13]  T. Waldmann,et al.  α-Fetoprotein in Nonneoplastic Hepatic Disorders , 1975 .

[14]  G. Karvountzis,et al.  Relation of alpha-fetoprotein in acute hepatitis to severity and prognosis. , 1974, Annals of internal medicine.