Regulation of type I collagen mRNA levels in fibroblasts.

Type I procollagen mRNA levels, as well as total RNA and poly(A)-rich mRNA, remain constant when rapidly growing human fetal lung fibroblasts (HFL-1 cells) are compared with quiescent cells. Polysome profiles of cells in both growth states revealed that the distribution of type I collagen mRNA in the mRNP fraction and in polysomes also remained constant even though total RNA and poly(A)-rich mRNA were shifted from polysomes to the mRNP pool in resting cells. Similar results were obtained when RNA fractions in polysomes associated with the cytoskeletal framework were examined. It is known that procollagen production is unaffected by the growth state of cells [Breul, S. D., Bradley, K. H., Hance, A. J., Schafer, M. P., Berg, R. A. and Crystal, R. G. (1980) J. Biol. Chem. 255, 5250-5260] although total protein synthesis is markedly decreased in resting cells. It would therefore appear that the translational control responsible for reduced synthesis of non-collagenous proteins in resting cells does not extend to procollagen and that transcriptional control can account for levels of type I procollagen produced by cultured human fibroblasts.

[1]  M. Bernard,et al.  Fine structural analysis of the human pro-alpha 1 (I) collagen gene. Promoter structure, AluI repeats, and polymorphic transcripts. , 1985, The Journal of biological chemistry.

[2]  M. Stephenson,et al.  Immune interferon inhibits collagen synthesis by rheumatoid synovial cells associated wth decreaded levels of the procollagen mRNAs(FEBS 2156) , 1985 .

[3]  J. Seyer,et al.  Transcriptional regulation of type I collagen genes in cultured fibroblasts by a factor isolated from thioacetamide-induced fibrotic rat liver. , 1984, The Journal of biological chemistry.

[4]  D. Melton,et al.  Efficient in vitro synthesis of biologically active RNA and RNA hybridization probes from plasmids containing a bacteriophage SP6 promoter. , 1984, Nucleic acids research.

[5]  J. Rosenbloom,et al.  Transcriptional control of human diploid fibroblast collagen synthesis by γ-interferon , 1984 .

[6]  L. Wasserman,et al.  Myelofibrosis and the biology of connective tissue , 1984 .

[7]  B. Geiger.  Membrane-cytoskeleton interaction. , 1983, Biochimica et biophysica acta.

[8]  P. Müller,et al.  Procollagen alpha 2(I) mRNA in dermatosparactic fibroblasts: evidence for post‐transcriptional regulation. , 1983, The EMBO journal.

[9]  S. Goelz,et al.  The role of the cytoskeleton in eukaryotic protein synthesis. (A minireview). , 1983, Cell biology international reports.

[10]  W. Jeffery,et al.  The cytoskeletal framework of sea urchin eggs and embryos: developmental changes in the association of messenger RNA. , 1983, Developmental biology.

[11]  J. Vuust,et al.  Control of Type I Collagen Systhesis: Evidence for Pretranslational Coordination of Proα1 (I) and Proα2 (I) Chain Synthesis in Embryonic Chick Bone , 1983 .

[12]  J. Myers,et al.  Cloning and characterization of five overlapping cDNAs specific for the human pro alpha 1(I) collagen chain. , 1982, Nucleic acids research.

[13]  T. Krieg,et al.  Influence of cell density on collagen biosynthesis in fibroblast cultures. , 1982, The Biochemical journal.

[14]  S. Faro,et al.  Cloning a cDNA for the pro-alpha 2 chain of human type I collagen. , 1981, Proceedings of the National Academy of Sciences of the United States of America.

[15]  P. Bornstein,et al.  Coordinate transcriptional regulation of type I procollagen genes by Rous sarcoma virus. , 1981, The Journal of biological chemistry.

[16]  I. Pastan,et al.  Regulation or procollagen messenger ribonucleic acid levels in Rous sarcoma virus transformed chick embryo fibroblasts. , 1981, Biochemistry.

[17]  R. Crystal,et al.  Procollagen production and procollagen messenger RNA levels and activity in human lung fibroblasts during periods of rapid and stationary growth. , 1981, The Journal of biological chemistry.

[18]  G. Dreyfuss,et al.  Messenger RNA is translated when associated with the cytoskeletal framework in normal and VSV-infected HeLa cells , 1981, Cell.

[19]  A. Infante,et al.  Distribution of messenger ribonucleic acid in polysomes and nonpolysomal particles of sea urchin embryos: translational control of actin synthesis. , 1981, Biochemistry.

[20]  R. Crystal,et al.  Control of collagen production by human diploid lung fibroblasts. , 1980, The Journal of biological chemistry.

[21]  K. Paigen,et al.  A simple, rapid, and sensitive DNA assay procedure. , 1980, Analytical biochemistry.

[22]  R. Glanville,et al.  Inhibiting effect of procollagen peptides on collagen biosynthesis in fibroblast cultures. , 1979, The Journal of biological chemistry.

[23]  P. Bornstein,et al.  Declining procollagen mRNA sequences in chick embryo fibroblasts infected with rous sarcoma virus. Correlation with procollagen synthesis. , 1979, The Journal of biological chemistry.

[24]  R. Palmiter,et al.  Correlation of procollagen mRNA levels in normal and transformed chick embryo fibroblasts with different rates of procollagen synthesis. , 1978, Biochemistry.

[25]  I. Pastan,et al.  Levels of translatable mRNAs for cell surface protein, collagen precursors, and two membrane proteins are altered in Rous sarcoma virus-transformed chick embryo fibroblasts. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[26]  P. Rudland,et al.  Changes in RNA metabolism and accumulation of presumptive messenger RNA during transition from the growing to the quiescent state of cultured mouse fibroblasts. , 1975, Journal of molecular biology.

[27]  P. Rudland.  Control of translation in cultured cells: continued synthesis and accumulation of messenger RNA in nondividing cultures. , 1974, Proceedings of the National Academy of Sciences of the United States of America.