论文信息 - Optogenetics meets optical wavefront shaping

Optogenetics meets optical wavefront shaping

Optogenetic stimulation by ultrashort laser pulses could allow neural circuits in the living brain to be probed with cellular resolution, despite pervasive light scattering. Now sophisticated new multiphoton stimulation systems that strike a better balance between lateral and axial resolution help realize this potential by matching the illumination volume to the soma's dimensions.

Shy Shoham | S. Shoham

[1] D. Tank,et al. Two-photon excitation of channelrhodopsin-2 at saturation , 2009, Proceedings of the National Academy of Sciences.

[2] Benjamin F. Grewe,et al. High-speed in vivo calcium imaging reveals neuronal network activity with near-millisecond precision , 2010, Nature Methods.

[3] Christoph Lutz,et al. Holographic photolysis of caged neurotransmitters , 2008, Nature Methods.

[4] Rafael Yuste,et al. Two-photon photostimulation and imaging of neural circuits , 2007, Nature Methods.

[5] S. Bernet,et al. Near-perfect hologram reconstruction with a spatial light modulator. , 2008, Optics express.

[6] Y. Silberberg,et al. Scanningless depth-resolved microscopy. , 2005, Optics express.

[7] Jesper Glückstad. Phase contrast image synthesis , 1996 .

[8] N Farah,et al. Design and characteristics of holographic neural photo-stimulation systems , 2009, Journal of neural engineering.

[9] B. Zemelman,et al. Two-photon single-cell optogenetic control of neuronal activity by sculpted light , 2010, Proceedings of the National Academy of Sciences.

[10] E. Isacoff,et al. Scanless two-photon excitation of channelrhodopsin-2 , 2010, Nature Methods.

[11] Raag D. Airan,et al. Optogenetic interrogation of neural circuits: technology for probing mammalian brain structures , 2010, Nature Protocols.

[12] Shy Shoham,et al. Rapid neurotransmitter uncaging in spatially defined patterns , 2005, Nature Methods.