Effects of pH and temperature on the continuous production of amylolytic enzymes by bacillus amyloliquefaciens MIR‐41

Bacillus amyloliquefaciens MIR-41 produces three exocellular amylolytic enzymes: α-amylase, pullulanase and α-glucosidase. Physicochemical factors such as pH and/or temperature may affect the enzyme yield. Continuous culture at a dilution rate of 0.15 h -1 demonstrated that the maximum production of α-amylase and α-glucosidase occurred at 36 o C and pH 6.8 and of pullulanase at 44 o C and pH 5.6. The results indicated that combinations of temperature and pH might be used for the production of crude extracts of α-amylase or pullulanase

[1]  J. Svobodová,et al.  Membrane fluidity inBacillus subtilis. Validity of homeoviscous adaptation , 2008, Folia Microbiologica (Prague).

[2]  G. R. Castro,et al.  Amylolytic enzymes produced by Bacillus amyloliquefaciens MIR‐41 in batch and continuous culture , 2007 .

[3]  G. R. Castro,et al.  Simul taneous production of alpha and beta amylases byBacillus subtilis MIR-5 in batch and continuous culture , 2005, Biotechnology Letters.

[4]  A. H. Stouthamer,et al.  Effect of different limitations in chemostat cultures on growth and production of exocellular protease byBacillus licheniformis , 1986, Applied Microbiology and Biotechnology.

[5]  G. R. Castro,et al.  Protein measurement with neocuproine reactive , 1991 .

[6]  G. R. Castro,et al.  A system for the differentiation of some closely related Bacillus species , 1991 .

[7]  Fergus G. Priest,et al.  Products and Applications , 1989 .

[8]  M. Thirunavukkarasu,et al.  Synthesis and localization of α‐amylase and α‐glucosidase in Bacillus licheniformis grown in batch and continuous culture , 1985 .

[9]  S. J. Luenser.  Microbial enzymes for industrial sweetener production , 1983 .

[10]  S. J. Pirt,et al.  Principles of microbe and cell cultivation , 1975 .

[11]  J. Thornley,et al.  Sporulation in Bacillus subtilis. Theoretical and experimental studies in continuous culture systems. , 1970, Journal of general microbiology.

[12]  Maxims Schwartz,et al.  La maltodextrine phosphorylase d'Escherichia coli. , 1967 .

[13]  H. Bender,et al.  Pullulanase from Aerobacter aerogenes; production in a cell-bound state. Purification and properties of the enzyme. , 1966, Biochemical and biophysical research communications.