Hydrolysis of cellulose and wood powder treated with DMDHEU by a hydrolase enzyme complex, Fenton's reagent, and in a liquid culture of Trametes versicolor

Abstract Cellulose was treated with various concentrations of 1,3-dimethylol-4,5-dihydroxyethylene urea (DMDHEU) to obtain different DMDHEU contents in the cellulose preparation. The release of sugars due to hydrolysis by a cellulase enzyme complex clearly decreased with increasing DMDHEU content. When the cellulose preparation with the highest DMDHEU content was subjected to pretreatment with Fenton's reagent (Fe2+, H2O2), the sugar release increased 4.5-fold. Incubation of modified cellulose with only H2O2 did not increase sugar release by cellulase. Compared to cellulose, the sugar release from milled wood powder induced by cellulase was very low but still decreased due to treatment with DMDHEU. Sole incubation of wood powder with Fenton's reagent did barely cause more sugar release compared to the storage of wood powder in buffer, but when the pretreated wood was incubated with cellulase the sugar release was higher than without pretreatment. Release of sugar from untreated cellulose in liquid cultures of the white-rot fungus Trametes versicolor was detected after 7 days and increased linearly with incubation time. Sugar release from DMDHEU-treated cellulose was much lower and was not detectable before 21 days of incubation. Total protein content in the cultures was low, but was slightly higher in the cultures of untreated cellulose. Laccase activity was not different in the presence of untreated and DMDHEU-treated cellulose after 7 and 14 days, but increased with increasing DMDHEU content after 21 and 28 days.

[1]  C. Mai,et al.  Protection mechanisms of DMDHEU treated wood against white and brown rot fungi , 2009 .

[2]  Yanjun Xie,et al.  Weathering of uncoated and coated wood treated with methylated 1,3-dimethylol-4,5-dihydroxyethyleneurea (mDMDHEU) , 2008, Holz als Roh- und Werkstoff.

[3]  C. Mai,et al.  Determination of fungal activity in modified wood by means of micro-calorimetry and determination of total esterase activity , 2008, Applied Microbiology and Biotechnology.

[4]  S. Grelier,et al.  Modification of Fagus sylvatica L. with 1,3-dimethylol-4,5-dihydroxy ethylene urea (DMDHEU). Part 2: Pore size distribution determined by differential scanning calorimetry , 2008 .

[5]  Alessandro Gandini,et al.  Chemical Modification of Wood , 2008 .

[6]  J. Jellison,et al.  Fungal Decay of Wood : Soft Rot-Brown Rot-White Rot , 2008 .

[7]  C. Hill,et al.  Decay resistance of anhydride-modified Corsican pine sapwood exposed to the brown rot fungus Coniophora puteana , 2006 .

[8]  C. Hill,et al.  Wood Modification: Chemical, Thermal and Other Processes , 2006 .

[9]  Yanjun Xie,et al.  Weathering of wood modified with the N-methylol compound 1,3-dimethylol-4,5-dihydroxyethyleneurea , 2005 .

[10]  G. R. Williams,et al.  An investigation of cell wall micropore blocking as a possible mechanism for the decay resistance of anhydride modified wood , 2005 .

[11]  K. Minato,et al.  Chemical modification of wood by non-formaldehyde cross-linking reagents , 1995, Wood Science and Technology.

[12]  H. Militz,et al.  Treatment of timber with water soluble dimethylol resins to improve their dimensional stability and durability , 1993, Wood Science and Technology.

[13]  C. Hill,et al.  The biological effectiveness of wood modified with linear chain carboxylic acid anhydrides against Coniophora puteana , 2002, Holz als Roh- und Werkstoff.

[14]  E. Srebotnik,et al.  Degradation of nonphenolic lignin by the laccase/1-hydroxybenzotriazole system. , 2000, Journal of biotechnology.

[15]  T. Highley,et al.  Biotechnology in the study of brown- and white-rot decay. , 1998 .

[16]  J. Simonsen.  Lack of Dimensional Stability in Cross-Linked Wood-Polymer Composites , 1998 .

[17]  A. Ritschkoff,et al.  The effect of oxidative pretreatment on cellulose degradation by Poria placenta and Trichoderma reesei cellulases , 1997, Applied Microbiology and Biotechnology.

[18]  F. Green,et al.  Mechanism of Brown-Rot decay : Paradigm or paradox , 1997 .

[19]  J. Jellison,et al.  The role of cations in the biodegradation of wood by the brown rot fungi , 1997 .

[20]  S. Yusuf.  PROPERTIES ENHANCEMENT OF WOOD BY CROSS-LINGKING FORMATION AND ITS APPLICATION TO THE RECONSTITUTED WOOD PRODUCTS , 1996 .

[21]  J. Paul,et al.  Changes in the Size and Volume of Pores in Sweetgum Wood During Simultaneous Rot by Phanerochaete chrysosporium Burds. , 1993 .

[22]  Robert A. Blanchette,et al.  Microbial and Enzymatic Degradation of Wood and Wood Components , 2012, Springer Series in Wood Science.

[23]  M. Gold,et al.  OXIDATIVE DEGRADATION OF LIGNIN BY PHOTOCHEMICAL AND CHEMICAL RADICAL GENERATING SYSTEMS , 1983 .

[24]  R. Willson,et al.  Radical-cations as reference chromogens in kinetic studies of ono-electron transfer reactions: pulse radiolysis studies of 2,2′-azinobis-(3-ethylbenzthiazoline-6-sulphonate) , 1982 .

[25]  I. Chet,et al.  Effect of phenolic compounds and tannin on growth and laccase activity of Fomes annosus , 1981 .

[26]  B. Pettersson,et al.  Production , purification and partial characterization of 1,4-beta-glucosidase enzymes from Sporotrichum pulverulentum. , 1978, European journal of biochemistry.

[27]  J. Zeikus,et al.  Ligninolytic enzyme system of Phanaerochaete chrysosporium: synthesized in the absence of lignin in response to nitrogen starvation , 1978, Journal of bacteriology.

[28]  B. Pettersson,et al.  Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 2. Activities of the five endo-1,4-beta-glucanases towards carboxymethylcellulose. , 1975, European journal of biochemistry.

[29]  B. Pettersson,et al.  Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 3. Purification and physico-chemical characterization of an exo-1,4-beta-glucanase. , 1975, European journal of biochemistry.

[30]  B. Pettersson,et al.  Extracellular enzyme system utilized by the fungus Sporotrichum pulverulentum (Chrysosporium lignorum) for the breakdown of cellulose. 1. Separation, purification and physico-chemical characterization of five endo-1,4-beta-glucanases. , 1975, European journal of biochemistry.

[31]  J. W. Koenigs.  Hydrogen Peroxide and Iron: A Proposed System for Decomposition of Wood by Brown-rot Basidiomycetes , 1974 .

[32]  J. E. Stone,et al.  A structural model for the cell wall of water-swollen wood pulp fibres based on their accessibility to macromolecules , 1968 .

[33]  G. L. Miller.  Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar , 1959 .

[34]  O. H. Lowry,et al.  Protein measurement with the Folin phenol reagent. , 1951, The Journal of biological chemistry.

[35]  H. Fenton,et al.  LXXIII.—Oxidation of tartaric acid in presence of iron , 1894 .