The Valorization of Spent Coffee Ground Extract as a Prospective Insecticidal Agent against Some Main Key Pests of Phaseolus vulgaris in the Laboratory and Field

The exploitation of massive amounts of food and agro-waste represents a severe social, economic, and environmental issue. Under the growing demand for food products that are free of toxic synthetic insecticides, a methanolic extract of spent coffee grounds (SCGs), which represent the main byproduct of coffee production, was applied in the current study as a bioinsecticide against the main pests of the green bean: Spodoptera littoralis, Agrotis ipsilon, Bemisia tabaci, Empoasca fabae, and Aphis craccivora. A deterrent assay, contact bioassay, and lethal concentration analysis were performed to reveal the repellent, antifeedant, and oviposition deterrent effects. Parallel to the above-mentioned bioassays, the phytochemical composition of the methanolic SCG extract was investigated via a high-performance liquid chromatography (HPLC) analysis. Fourteen phenolic acids and five flavonoids, in addition to caffeine (alkaloid), were identified in the extract. Cinnamic, rosmarinic, and gallic acids were the predominant phenolics, while apigenin-7-glucoside was the main flavonoid, followed by naringin, catechin, and epicatechin. The extract of SCGs showed an insecticidal effect, with a mortality between 27.5 and 76% compared to the control (7.4%) and based on the concentration of the extract used. In the same trend, the oviposition efficiency revealed different batches of laid eggs (0.67, 2.33, 7.33, and 8.67 batches/jar) for 100, 50, and 25% of the SCG extract and the control. Finally, the major components of the SCG extract were docked into the insecticide acetylcholinesterase enzyme to explore their potential for inhibition, where apigenin-7-glucoside showed a higher binding affinity, followed by catechin, compared to the control (lannate). The obtained findings could be a starting point for developing novel bioinsecticides from SCGs.

[1]  A. Badr,et al.  Bioactivity evaluation for volatiles and water extract of commercialized star anise , 2021, Heliyon.

[2]  A. Seca,et al.  Aqueous and Ethanolic Plant Extracts as Bio-Insecticides—Establishing a Bridge between Raw Scientific Data and Practical Reality , 2021, Plants.

[3]  H. Alpas,et al.  Improving the Recovery of Phenolic Compounds from Spent Coffee Grounds (SCG) by Environmentally Friendly Extraction Techniques , 2021, Molecules.

[4]  Dilip Shriram Ghongade,et al.  Efficacy of biopesticides against the whitefly, Bemisia tabaci (Gennadius) (Hemiptera: Aleyrodidae), on parthenocarpic cucumber grown under protected environment in India , 2021 .

[5]  Chung-Ho Lin,et al.  Evaluation of fatty acids, phenolics and bioactivities of spent coffee grounds prepared from Vietnamese coffee , 2021, International Journal of Food Properties.

[6]  I. Adejumo,et al.  Agricultural Solid Wastes: Causes, Effects, and Effective Management , 2020, Strategies of Sustainable Solid Waste Management.

[7]  N. Uslu,et al.  Effect of microwave and oven roasting methods on total phenol, antioxidant activity, phenolic compounds, and fatty acid compositions of coffee beans , 2020 .

[8]  E. Vamanu,et al.  Bioavailability and Bioactivities of Polyphenols Eco Extracts from Coffee Grounds after In Vitro Digestion , 2020, Foods.

[9]  G. Zengin,et al.  Chemical Composition, Antioxidant and Enzyme Inhibitory Properties of Different Extracts Obtained from Spent Coffee Ground and Coffee Silverskin , 2020, Foods.

[10]  S. Vittori,et al.  An analytical method for the simultaneous quantification of 30 bioactive compounds in spent coffee ground by HPLC-MS/MS. , 2020, Journal of mass spectrometry : JMS.

[11]  Zubir,et al.  Effect of Coffee Grounds as Cockroach Repellent , 2020 .

[12]  A. Leksono,et al.  The active compounds composition and antifeedant activity of leaf extract of two cultivar Carica papaya L. on Spodoptera litura F. larvae , 2020 .

[13]  C. Palanuvej,et al.  Quantification of chlorogenic acid, rosmarinic acid, and caffeic acid contents in selected Thai medicinal plants using RP-HPLC-DAD , 2020 .

[14]  P. Lucci,et al.  Spent espresso coffee grounds as a source of anti-proliferative and antioxidant compounds , 2020, Innovative Food Science & Emerging Technologies.

[15]  Andrew Newton,et al.  London, UK , 2020 .

[16]  S. Attia,et al.  EGYPTIAN DEMAND FOR FABA BEANS FROM THE MOST IMPORTANT INTERNATIONAL IMPORT MARKETS , 2019, Arab Universities Journal of Agricultural Sciences.

[17]  C. H. Tomich de Paula da Silva,et al.  Identification of Potential Inhibitors from Pyriproxyfen with Insecticidal Activity by Virtual Screening , 2019, Pharmaceuticals.

[18]  Mendes Soares Ilsamar,et al.  Chemical composition, oviposition deterrent and larvicidal activities of the wood extracts of Tabebuia avellanedae from the Cerrado of Brazil , 2018, Journal of Medicinal Plants Research.

[19]  A. Kovalcik,et al.  Valorization of spent coffee grounds: A review , 2018, Food and Bioproducts Processing.

[20]  Gianni De Fabritiis,et al.  DeepSite: protein‐binding site predictor using 3D‐convolutional neural networks , 2017, Bioinform..

[21]  P. Enck,et al.  The potential effects of chlorogenic acid, the main phenolic components in coffee, on health: a comprehensive review of the literature , 2017, European Journal of Nutrition.

[22]  R. Campos-Vega,et al.  Spent coffee grounds, an innovative source of colonic fermentable compounds, inhibit inflammatory mediators in vitro. , 2016, Food chemistry.

[23]  J. Zygadlo,et al.  Terpenes: Natural Products for Controlling Insects of Importance to Human Health—A Structure-Activity Relationship Study , 2016 .

[24]  L. Teh,et al.  Identification of novel acetylcholinesterase inhibitors: Indolopyrazoline derivatives and molecular docking studies. , 2016, Bioorganic chemistry.

[25]  O. B. Kovanci Feeding and oviposition deterrent activities of microencapsulated cardamom oleoresin and eucalyptol against Cydia pomonella , 2016 .

[26]  D. S. Alves,et al.  Malpighia emarginata DC. bagasse acetone extract: Phenolic compounds and their effect on Spodoptera frugiperda (J.E. Smith) (Lepidoptera: Noctuidae) , 2016 .

[27]  H. Sytykiewicz,et al.  APHIDICIDAL AND DETERRENT ACTIVITY OF PHENOLIC ACID EXTRACTS FROM SOME HERBAL PLANTS TOWARDS MYZUS PERSICAE SULZ . AND RHOPALOSIPHUM PADI L , 2016 .

[28]  P. Ndakidemi,et al.  Extracts from Field Margin Weeds Provide Economically Viable and Environmentally Benign Pest Control Compared to Synthetic Pesticides , 2015, PloS one.

[29]  C. Nolasco-Hipólito,et al.  Coffee and its waste repel gravid Aedes albopictus females and inhibit the development of their embryos , 2015, Parasites & Vectors.

[30]  A. Basukriadi,et al.  Oviposition Deterrent Activities of Pachyrhizus erosus Seed Extract and Other Natural Products on Plutella xylostella (Lepidoptera: Plutellidae) , 2014, Journal of insect science.

[31]  Marcus D. Hanwell,et al.  Avogadro: an advanced semantic chemical editor, visualization, and analysis platform , 2012, Journal of Cheminformatics.

[32]  M. Bonesi,et al.  Antioxidant and Anti-cholinesterase Activity of Globularia meridionalis Extracts and Isolated Constituents , 2012, Natural product communications.

[33]  H. Sytykiewicz,et al.  Effect of phenolic acids from black currant, sour cherry and walnut on grain aphid (Sitobion avenae F.) development , 2012 .

[34]  M. Maraschin,et al.  Supercritical fluid extraction from spent coffee grounds and coffee husks: antioxidant activity and effect of operational variables on extract composition. , 2012, Talanta.

[35]  S. Mussatto,et al.  Extraction of antioxidant phenolic compounds from spent coffee grounds , 2011 .

[36]  R. Pavela Antifeedant and Larvicidal Effects of Some Phenolic Components of Essential Oils Lasp Lines of Introduction Against Spodoptera littoralis (Boisd.) , 2011 .

[37]  SinghShree,et al.  Review: Breeding common bean for resistance to insect pests and nematodes , 2010 .

[38]  David S. Goodsell,et al.  AutoDock4 and AutoDockTools4: Automated docking with selective receptor flexibility , 2009, J. Comput. Chem..

[39]  Wei Zhao,et al.  Validation of Molecular Docking Programs for Virtual Screening against Dihydropteroate Synthase , 2009, J. Chem. Inf. Model..

[40]  S. Cui,et al.  Phenolic acid profiles and antioxidant activities of wheat bran extracts and the effect of hydrolysis conditions , 2006 .

[41]  H. E. Bicudo,et al.  Effects of caffeine and used coffee grounds on biological features of Aedes aegypti (Diptera, Culicidae) and their possible use in alternative control , 2003 .

[42]  C. Vance,et al.  Legumes: Importance and Constraints to Greater Use , 2003, Plant Physiology.

[43]  A. Mutero,et al.  Drosophila acetylcholinesterase: mechanisms of resistance to organophosphates. , 1993, Chemico-biological interactions.