Controlling the enantioselectivity of enzymes by directed evolution: practical and theoretical ramifications.
暂无分享,去创建一个
[1] Marc Ostermeier,et al. A combinatorial approach to hybrid enzymes independent of DNA homology , 1999, Nature Biotechnology.
[2] L. Pauling,et al. Nature of Forces between Large Molecules of Biological Interest , 1948, Nature.
[3] C. Mak,et al. Directed evolution of D-2-keto-3-deoxy-6-phosphogluconate aldolase to new variants for the efficient synthesis of D- and L-sugars. , 2000, Chemistry & biology.
[4] M. Nardini,et al. Directed evolution of an enantioselective lipase. , 2000, Chemistry & biology.
[5] K. Faber,et al. Stereoselectivities of microbial epoxide hydrolases. , 1999, Current opinion in chemical biology.
[6] Frances H. Arnold,et al. Directed enzyme evolution : screening and selection methods , 2003 .
[7] M. Reetz. Directed evolution of enantioselective enzymes as catalysts in the production of chiral pharmaceuticals , 2002 .
[8] Frances H. Arnold,et al. Inverting enantioselectivity by directed evolution of hydantoinase for improved production of l-methionine , 2000, Nature Biotechnology.
[9] G. F. Joyce,et al. Randomization of genes by PCR mutagenesis. , 1992, PCR methods and applications.
[10] P. Agarwal,et al. Network of coupled promoting motions in enzyme catalysis , 2002, Proceedings of the National Academy of Sciences of the United States of America.
[11] Manfred T. Reetz,et al. Directed evolution of selective enzymes and hybrid catalysts , 2002 .
[12] A. Svendsen. Enzyme Functionality : Design: Engineering, and Screening , 2003 .
[13] B. List. Proline-catalyzed asymmetric reactions , 2002 .
[14] H. Stunnenberg,et al. Improved method for PCR-mediated site-directed mutagenesis. , 1994, Nucleic acids research.
[15] Manfred T. Reetz,et al. Towards the directed evolution of hybrid catalysts , 2002 .
[16] N. Oppenheimer,et al. Structure and mechanism , 1989 .
[17] F. Christians,et al. Tolerance of different proteins for amino acid diversity , 1996, Molecular Diversity.
[18] F. Cédrone,et al. Directed Evolution of the Epoxide Hydrolase from Aspergillus niger , 2003 .
[19] M. Reetz,et al. Second-generation MS-based high-throughput screening system for enantioselective catalysts and biocatalysts , 2002 .
[20] Andrea Zocchi,et al. Artificial metalloenzymes for enantioselective catalysis based on biotin-avidin. , 2003, Journal of the American Chemical Society.
[21] M. Reetz. Lipases as practical biocatalysts. , 2002, Current opinion in chemical biology.
[22] Nicholas J Turner,et al. Directed evolution of an amine oxidase possessing both broad substrate specificity and high enantioselectivity. , 2003, Angewandte Chemie.
[23] E. Jacobsen,et al. Asymmetric catalysis of epoxide ring-opening reactions. , 2000, Accounts of chemical research.
[24] Matthias Boese,et al. A practical high-throughput screening system for enantioselectivity by using FTIR spectroscopy. , 2003, Chemistry.
[25] F. Arnold,et al. How enzymes adapt: lessons from directed evolution , 2001, Trends in biochemical sciences.
[26] Manfred T. Reetz,et al. Creation of Enantioselective Biocatalysts for Organic Chemistry by In Vitro Evolution , 1997 .
[27] E. Fischer. Einfluss der Configuration auf die Wirkung der Enzyme , 1894 .
[28] Ryoji Noyori Prof.. Asymmetric Catalysis: Science and Opportunities (Nobel Lecture) , 2002 .
[29] A. Klibanov. Improving enzymes by using them in organic solvents , 2001, Nature.
[30] M. Distefano,et al. Generation of new enzymes via covalent modification of existing proteins. , 2001, Chemical reviews.
[31] Manfred T Reetz,et al. A Method for High-Throughput Screening of Enantioselective Catalysts. , 1999, Angewandte Chemie.
[32] Keith A. Powell,et al. Directed Evolution and Biocatalysis. , 2001, Angewandte Chemie.
[33] U. Bornscheuer,et al. Directed Evolution of an Esterase from Pseudomonas fluorescens. Random Mutagenesis by Error-Prone PCR or a Mutator Strain and Identification of Mutants Showing Enhanced Enantioselectivity by a Resorufin-Based Fluorescence Assay , 1999, Biological chemistry.
[34] Ryoji Noyori,et al. Asymmetric catalysis: science and opportunities (Nobel lecture). , 2002, Angewandte Chemie.
[35] George M. Whitesides,et al. Conversion of a protein to a homogeneous asymmetric hydrogenation catalyst by site-specific modification with a diphosphinerhodium(I) moiety , 1978 .
[36] Kai Johnsson,et al. Directed Molecular Evolution of Proteins or How to Improve Enzymes for Biocatalysis , 2002 .
[37] D. Hilvert,et al. Semisynthetic enzymes: design of flavin-dependent oxidoreductases. , 1987, Biotechnology & genetic engineering reviews.
[38] K. Sharpless,et al. Searching for new reactivity (Nobel lecture). , 2002, Angewandte Chemie.
[39] W. Knowles. Asymmetric hydrogenations (Nobel lecture). , 2002, Angewandte Chemie.
[40] Manfred T. Reetz,et al. A Practical NMR‐Based High‐Throughput Assay for Screening Enantioselective Catalysts and Biocatalysts , 2002 .
[41] M. Reetz. Application of directed evolution in the development of enantioselective enzymes , 2000 .
[42] K. Achiwa,et al. INVERSION OF ENANTIOSELECTIVITY IN HYDROLYSIS OF 1,4-DIHYDROPYRIDINES BY POINT MUTATION OF LIPASE PS , 1995 .
[43] Yvonne Genzel,et al. Enhancing the enantioselectivity of an epoxide hydrolase by directed evolution. , 2004, Organic letters.
[44] Eric N. Jacobsen,et al. Comprehensive asymmetric catalysis , 1999 .
[45] Volker Sieber,et al. Libraries of hybrid proteins from distantly related sequences , 2001, Nature Biotechnology.
[46] Karlheinz Drauz,et al. Enzyme Catalysis in Organic Synthesis: A Comprehensive Handbook: Vol. 1 J. Am. Chem. Soc. 1996, 118, 11340 , 1997 .
[47] Manfred T Reetz,et al. An overview of high-throughput screening systems for enantioselective enzymatic transformations. , 2003, Methods in molecular biology.
[48] F. Arnold. Combinatorial and computational challenges for biocatalyst design , 2001, Nature.
[49] F. Arnold,et al. Directed evolution converts subtilisin E into a functional equivalent of thermitase. , 1999, Protein engineering.
[50] R. Lerner,et al. The chemistry of the antibody molecule. , 2002, Angewandte Chemie.
[51] M. Reetz,et al. Superior Biocatalysts by Directed Evolution , 1999 .
[52] C. Saudan,et al. Directed molecular evolution of cytochrome c peroxidase. , 2000, Biochemistry.
[53] A. Fersht. Structure and mechanism in protein science , 1998 .
[54] J. Short,et al. A Novel, High Performance Enzyme for Starch Liquefaction , 2002, The Journal of Biological Chemistry.
[55] Donald Hilvert,et al. Investigating and Engineering Enzymes by Genetic Selection. , 2001, Angewandte Chemie.
[56] K. Hult,et al. Improved Enantioselectivity of a Lipase by Rational Protein Engineering , 2001, Chembiochem : a European journal of chemical biology.
[57] Manfred T Reetz,et al. Assembly of Designed Oligonucleotides as an Efficient Method for Gene Recombination: A New Tool in Directed Evolution , 2003, Chembiochem : a European journal of chemical biology.
[58] M. Burk,et al. Creation of a productive, highly enantioselective nitrilase through gene site saturation mutagenesis (GSSM). , 2003, Journal of the American Chemical Society.
[59] M. Nardini,et al. University of Groningen Crystal structure of Pseudomonas aeruginosa lipase in the open conformation-The prototype for family I.1 of bacterial lipases Nardini, , 2000 .
[60] R. Hosur,et al. Effects of remote mutation on the autolysis of HIV-1 PR: X-ray and NMR investigations. , 2002, Biochemical and biophysical research communications.
[61] A. Crameri,et al. Combinatorial multiple cassette mutagenesis creates all the permutations of mutant and wild-type sequences. , 1995, BioTechniques.
[62] Manfred T. Reetz,et al. Directed Evolution of an Enantioselective Enzyme through Combinatorial Multiple-Cassette Mutagenesis. , 2001, Angewandte Chemie.
[63] M. Reetz. Combinatorial and Evolution-Based Methods in the Creation of Enantioselective Catalysts. , 2001, Angewandte Chemie.