Advances in laboratory evolution of enzymes.

[1]  M. Huynen,et al.  Neutral evolution of mutational robustness. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[2]  E. Bornberg-Bauer,et al.  Modeling evolutionary landscapes: mutational stability, topology, and superfunnels in sequence space. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[3]  J. H. Shim,et al.  Combinatorial protein engineering by incremental truncation. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[4]  W. Fontana,et al.  Plasticity, evolvability, and modularity in RNA. , 2000, The Journal of experimental zoology.

[5]  M. Lehmann,et al.  From DNA sequence to improved functionality: using protein sequence comparisons to rapidly design a thermostable consensus phytase. , 2000, Protein engineering.

[6]  M Wilmanns,et al.  Directed evolution of a (beta alpha)8-barrel enzyme to catalyze related reactions in two different metabolic pathways. , 2000, Proceedings of the National Academy of Sciences of the United States of America.

[7]  F. Arnold,et al.  How enzymes adapt: lessons from directed evolution , 2001, Trends in biochemical sciences.

[8]  Manel Camps,et al.  Targeted gene evolution in Escherichia coli using a highly error-prone DNA polymerase I , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[9]  Jean-Louis Reymond,et al.  Enzyme assays for high-throughput screening. , 2004, Current opinion in biotechnology.

[10]  D. Bamford,et al.  Evolutionary Potential of an RNA Virus , 2004, Journal of Virology.

[11]  K. Wittrup,et al.  Shuffled antibody libraries created by in vivo homologous recombination and yeast surface display. , 2004, Nucleic acids research.

[12]  Dan S. Tawfik,et al.  Directed evolution of mammalian paraoxonases PON1 and PON3 for bacterial expression and catalytic specialization. , 2004, Proceedings of the National Academy of Sciences of the United States of America.

[13]  R. Kazlauskas,et al.  Improving enzyme properties: when are closer mutations better? , 2005, Trends in biotechnology.

[14]  A. Griffiths,et al.  High-throughput screening of enzyme libraries: in vitro evolution of a beta-galactosidase by fluorescence-activated sorting of double emulsions. , 2005, Chemistry & biology.

[15]  Dan S. Tawfik,et al.  The 'evolvability' of promiscuous protein functions , 2005, Nature Genetics.

[16]  George Georgiou,et al.  Engineering of protease variants exhibiting high catalytic activity and exquisite substrate selectivity. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[17]  Peter L Bergquist,et al.  Degenerate oligonucleotide gene shuffling (DOGS) and random drift mutagenesis (RNDM): two complementary techniques for enzyme evolution. , 2005, Biomolecular engineering.

[18]  P. Alexander,et al.  Directed coevolution of stability and catalytic activity in calcium-free subtilisin. , 2005, Biochemistry.

[19]  Dan S. Tawfik,et al.  High-throughput screening of enzyme libraries: thiolactonases evolved by fluorescence-activated sorting of single cells in emulsion compartments. , 2005, Chemistry & biology.

[20]  Benny K. C. Lo,et al.  Generating molecular diversity by homologous recombination in Escherichia coli. , 2005, Protein engineering, design & selection : PEDS.

[21]  Stephen J Benkovic,et al.  Evolution of highly active enzymes by homology-independent recombination. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[22]  Motowo Nakajima,et al.  Biased mutation-assembling: an efficient method for rapid directed evolution through simultaneous mutation accumulation. , 2005, Protein engineering, design & selection : PEDS.

[23]  Peter T. Lansbury,et al.  A computer program for the estimation of protein and nucleic acid sequence diversity in random point mutagenesis libraries , 2005, Nucleic acids research.

[24]  Mats Holmquist,et al.  Focusing mutations into the P. fluorescens esterase binding site increases enantioselectivity more effectively than distant mutations. , 2005, Chemistry & biology.

[25]  Dan S. Tawfik,et al.  Evolution of new protein topologies through multistep gene rearrangements , 2006, Nature Genetics.

[26]  Rohit Sharma,et al.  Directed Evolution: An Approach to Engineer Enzymes , 2006, Critical reviews in biotechnology.

[27]  Amir Aharoni,et al.  High-throughput screening methodology for the directed evolution of glycosyltransferases , 2006, Nature Methods.

[28]  W. Patrick,et al.  Natural history as a predictor of protein evolvability. , 2006, Protein engineering, design & selection : PEDS.

[29]  Dan S. Tawfik,et al.  Enzyme promiscuity: evolutionary and mechanistic aspects. , 2006, Current opinion in chemical biology.

[30]  Manfred T Reetz,et al.  Designing new Baeyer-Villiger monooxygenases using restricted CASTing. , 2006, The Journal of organic chemistry.

[31]  Dan S. Tawfik,et al.  Robustness–epistasis link shapes the fitness landscape of a randomly drifting protein , 2006, Nature.

[32]  Frances H. Arnold,et al.  Structure-guided SCHEMA recombination of distantly related β-lactamases , 2006 .

[33]  Andrew D Griffiths,et al.  Miniaturising the laboratory in emulsion droplets. , 2006, Trends in biotechnology.

[34]  F. Arnold,et al.  Protein stability promotes evolvability. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[35]  Jared R. Leadbetter,et al.  Dual selection enhances the signaling specificity of a variant of the quorum-sensing transcriptional activator LuxR , 2006, Nature Biotechnology.

[36]  Marco A Mena,et al.  Blue fluorescent proteins with enhanced brightness and photostability from a structurally targeted library , 2006, Nature Biotechnology.

[37]  Jay H. Lee,et al.  Pooling for Improved Screening of Combinatorial Libraries for Directed Evolution , 2006, Biotechnology progress.

[38]  Ryota Fujii,et al.  RAISE: a simple and novel method of generating random insertion and deletion mutations , 2006, Nucleic acids research.

[39]  George Georgiou,et al.  The evolution of catalytic efficiency and substrate promiscuity in human theta class 1-1 glutathione transferase. , 2006, Journal of molecular biology.

[40]  Ulrich Schwaneberg,et al.  A screening system for the directed evolution of epoxygenases: importance of position 184 in P450 BM3 for stereoselective styrene epoxidation. , 2006, Angewandte Chemie.

[41]  Huimin Zhao,et al.  Directed evolution of enzymes and biosynthetic pathways. , 2006, Current opinion in microbiology.

[42]  Thomas E. Ferrin,et al.  Designed divergent evolution of enzyme function , 2006, Nature.

[43]  Ranjini Chatterjee,et al.  Directed evolution of metabolic pathways. , 2006, Trends in biotechnology.

[44]  Martin Zacharias,et al.  A statistical analysis of random mutagenesis methods used for directed protein evolution. , 2006, Journal of molecular biology.

[45]  Andrew D Griffiths,et al.  Directed evolution by in vitro compartmentalization , 2006, Nature Methods.

[46]  Huimin Zhao,et al.  In vitro 'sexual' evolution through the PCR-based staggered extension process (StEP) , 2006, Nature Protocols.

[47]  H. Leemhuis,et al.  Conversion of a cyclodextrin glucanotransferase into an alpha-amylase , 2018 .

[48]  Tuck Seng Wong,et al.  Steering directed protein evolution: strategies to manage combinatorial complexity of mutant libraries. , 2007, Environmental microbiology.

[49]  Karen M Polizzi,et al.  Stability of biocatalysts. , 2007, Current opinion in chemical biology.

[50]  A. Dean,et al.  Mechanistic approaches to the study of evolution: the functional synthesis , 2007, Nature Reviews Genetics.

[51]  Gavin J. Williams,et al.  Expanding the promiscuity of a natural-product glycosyltransferase by directed evolution. , 2007, Nature chemical biology.

[52]  Dan S. Tawfik,et al.  Protein engineers turned evolutionists , 2007, Nature Methods.

[53]  Manfred T Reetz,et al.  Iterative saturation mutagenesis (ISM) for rapid directed evolution of functional enzymes , 2007, Nature Protocols.

[54]  Manel Camps,et al.  Genetic Constraints on Protein Evolution , 2007, Critical reviews in biochemistry and molecular biology.

[55]  Dan S. Tawfik,et al.  Latent evolutionary potentials under the neutral mutational drift of an enzyme , 2007 .

[56]  F. Matsumura,et al.  Rapid activation of c-Src kinase by dioxin is mediated by the Cdc37-HSP90 complex as part of Ah receptor signaling in MCF10A cells. , 2007, Biochemistry.

[57]  Homme W Hellinga,et al.  Protein fabrication automation , 2007, Protein science : a publication of the Protein Society.

[58]  D. D. Jones,et al.  Investigating protein structural plasticity by surveying the consequence of an amino acid deletion from TEM‐1 β‐lactamase , 2007, FEBS letters.

[59]  Alpan Raval,et al.  Evolution favors protein mutational robustness in sufficiently large populations , 2007 .

[60]  S. Pääbo,et al.  Molecular breeding of polymerases for amplification of ancient DNA , 2007, Nature Biotechnology.

[61]  Elizabeth M J Gillam,et al.  Extending the diversity of cytochrome P450 enzymes by DNA family shuffling. , 2007, Gene.

[62]  Frances H Arnold,et al.  Neutral genetic drift can aid functional protein evolution , 2007, 0705.0201.

[63]  Yu Zheng,et al.  Selection of restriction endonucleases using artificial cells , 2007, Nucleic acids research.

[64]  W. Quax,et al.  Selection strategies for improved biocatalysts , 2007, The FEBS journal.

[65]  K Rumbold,et al.  A novel screening assay for hydroxynitrile lyases suitable for high-throughput screening. , 2007, Journal of biotechnology.

[66]  Burckhard Seelig,et al.  Selection and evolution of enzymes from a partially randomized non-catalytic scaffold , 2007, Nature.

[67]  Dan S. Tawfik,et al.  Reconstruction of functional beta-propeller lectins via homo-oligomeric assembly of shorter fragments. , 2007, Journal of molecular biology.

[68]  T. Yamane,et al.  Creation of novel enantioselective lipases by SIMPLEX. , 2007, Methods in molecular biology.

[69]  B. Tidor,et al.  Selection of horseradish peroxidase variants with enhanced enantioselectivity by yeast surface display. , 2007, Chemistry & biology.

[70]  J. Hiratake,et al.  Engineering of Pseudomonas aeruginosa lipase by directed evolution for enhanced amidase activity: mechanistic implication for amide hydrolysis by serine hydrolases. , 2007, Protein engineering, design & selection : PEDS.

[71]  The M.EcoRV DNA-(Adenine N6)-methyltransferase Uses DNA Bending for Recognition of an Expanded EcoDam Recognition Site* , 2007, Journal of Biological Chemistry.

[72]  V. V. Dasu,et al.  Developments in Directed Evolution for Improving Enzyme Functions , 2007, Applied biochemistry and biotechnology.

[73]  S. Kurtovic,et al.  Multivariate-activity mining for molecular quasi-species in a glutathione transferase mutant library. , 2007, Protein engineering, design & selection : PEDS.

[74]  D. Hilvert,et al.  Metabolic engineering of a genetic selection system with tunable stringency , 2007, Proceedings of the National Academy of Sciences.

[75]  Dan S. Tawfik,et al.  Incorporating Synthetic Oligonucleotides via Gene Reassembly (ISOR): a versatile tool for generating targeted libraries. , 2007, Protein engineering, design & selection : PEDS.

[76]  T. Stachelhaus,et al.  An optimized ATP/PP(i)-exchange assay in 96-well format for screening of adenylation domains for applications in combinatorial biosynthesis. , 2007, Biotechnology journal.

[77]  John C Whitman,et al.  Improving catalytic function by ProSAR-driven enzyme evolution , 2007, Nature Biotechnology.

[78]  Karl-Erich Jaeger,et al.  Ultrahigh‐Throughput Screening to Identify E. coli Cells Expressing Functionally Active Enzymes on their Surface , 2007, Chembiochem : a European journal of chemical biology.

[79]  Donald Hilvert,et al.  A simple selection strategy for evolving highly efficient enzymes , 2007, Nature Biotechnology.

[80]  S. Shleev,et al.  Altering the laccase functionality by in vivo assembly of mutant libraries with different mutational spectra , 2008, Proteins.