Argonaute and RNA--getting into the groove.

RNAi has made an enormous impact on biology in a very short period of time. It became an extraordinarily useful and simple tool for gene silencing, even as its fascinating mechanism was gradually unraveling. Understanding the mechanism of RNAi-related pathways, including both transcriptional and posttranscriptional gene silencing and even processes such as DNA elimination in Tetrahymena, have benefited from an incredible marriage of genetics, biochemistry, molecular biology, bioinformatics and finally structural biology. Structural biology played a key role in deciphering the role that a central player--the Argonaute protein--has in all RNAi processes.

[1]  A. Bateman,et al.  Domains in gene silencing and cell differentiation proteins: the novel PAZ domain and redefinition of the Piwi domain. , 2000, Trends in biochemical sciences.

[2]  Ji-Joon Song,et al.  Purified Argonaute2 and an siRNA form recombinant human RISC , 2005, Nature Structural &Molecular Biology.

[3]  Harrison W. Gabel,et al.  Functional Genomic Analysis of RNA Interference in C. elegans , 2005, Science.

[4]  Wei Yang,et al.  Crystal Structures of RNase H Bound to an RNA/DNA Hybrid: Substrate Specificity and Metal-Dependent Catalysis , 2005, Cell.

[5]  Michael Q. Zhang,et al.  The Argonaute family: tentacles that reach into RNAi, developmental control, stem cell maintenance, and tumorigenesis. , 2002, Genes & development.

[6]  J. M. Thomson,et al.  Argonaute2 Is the Catalytic Engine of Mammalian RNAi , 2004, Science.

[7]  Deborah S Wuttke,et al.  Nucleic acid recognition by OB-fold proteins. , 2003, Annual review of biophysics and biomolecular structure.

[8]  G. Hannon,et al.  Crystal Structure of Argonaute and Its Implications for RISC Slicer Activity , 2004, Science.

[9]  B. Simon,et al.  Structure and nucleic-acid binding of the Drosophila Argonaute 2 PAZ domain , 2003, Nature.

[10]  T. Tuschl,et al.  Crystal structure of A. aeolicus argonaute, a site-specific DNA-guided endoribonuclease, provides insights into RISC-mediated mRNA cleavage. , 2005, Molecular cell.

[11]  D. Patel,et al.  Structural basis for overhang-specific small interfering RNA recognition by the PAZ domain , 2004, Nature.

[12]  Akira Ishizuka,et al.  Distinct roles for Argonaute proteins in small RNA-directed RNA cleavage pathways. , 2004, Genes & development.

[13]  D. Barford,et al.  Structural insights into mRNA recognition from a PIWI domain–siRNA guide complex , 2005, Nature.

[14]  A A Antson,et al.  Single-stranded-RNA binding proteins. , 2000, Current opinion in structural biology.

[15]  Ji-Joon Song,et al.  The crystal structure of the Argonaute2 PAZ domain reveals an RNA binding motif in RNAi effector complexes , 2003, Nature Structural Biology.

[16]  N. Rhind,et al.  A single Argonaute protein mediates both transcriptional and posttranscriptional silencing in Schizosaccharomyces pombe. , 2004, Genes & development.

[17]  Michael Sattler,et al.  Nucleic acid 3′-end recognition by the Argonaute2 PAZ domain , 2004, Nature Structural &Molecular Biology.

[18]  Eric Westhof,et al.  Single Processing Center Models for Human Dicer and Bacterial RNase III , 2004, Cell.

[19]  Thomas Tuschl,et al.  Structural basis for 5′-end-specific recognition of guide RNA by the A. fulgidus Piwi protein , 2005, Nature.

[20]  Anton J. Enright,et al.  MicroRNA targets in Drosophila , 2003, Genome Biology.

[21]  Phillip D. Zamore,et al.  Ribo-gnome: The Big World of Small RNAs , 2005, Science.

[22]  G. Hannon,et al.  RNase III enzymes and the initiation of gene silencing , 2004, Nature Structural &Molecular Biology.

[23]  D. Baulcombe,et al.  Arabidopsis ARGONAUTE1 is an RNA Slicer that selectively recruits microRNAs and short interfering RNAs. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[24]  T. Steitz,et al.  Recombining the structures of HIV integrase, RuvC and RNase H. , 1995, Structure.

[25]  D. Barford,et al.  Crystal structure of a PIWI protein suggests mechanisms for siRNA recognition and slicer activity , 2004, The EMBO journal.