A multi‐functional electrochemical sensing system using microfluidic technology for the detection of urea and creatinine

This study presents a new microfluidic system capable of precise measurements of two important biomarkers, urea and creatinine, automatically. In clinical applications, high levels of these two biomarkers are early indicators of nephropathy or renal failure and should be monitored on a regular basis. The microfluidic system is composed of a microfluidic chip, a control circuit system, a compressed air source and several electromagnetic valves to form a handheld system. The microfluidic chip is fabricated by using micro‐electromechanical systems and microfluidic techniques comprising electrochemical sensor arrays and polydimethylsiloxane‐based microfluidic structures such as micropumps/micromixers, normally closed valves and microchannels. The microfluidic system performs a variety of critical processes including sample pretreatment, mixing, transportation and detection on a single chip. The experimental results show that the entire procedure takes approximately 40 min, which is much faster than the traditional method (more than 6 h). Furthermore, the total sample volume consumed in each operation is only 0.1 mL, which is significantly less than that required in a large system (5 mL). The developed automatic microfluidic system may provide a powerful platform for further clinical applications.

[1]  A. Manz,et al.  Miniaturized total chemical analysis systems: A novel concept for chemical sensing , 1990 .

[2]  Honglan Shi,et al.  A simple and fast method to determine and quantify urinary creatinine , 1995 .

[3]  L. Gorton,et al.  Development of enzyme-based amperometric sensors for the determination of phenolic compounds , 1995 .

[4]  Richard P. Buck,et al.  Microfabricated amperometric creatine and creatinine biosensors , 1996 .

[5]  A Bratov,et al.  Photosensitive polyurethanes applied to the development of CHEMFET and ENFET devices for biomedical sensing. , 1997, Biosensors & bioelectronics.

[6]  Eun Ju Kim,et al.  Disposable creatinine sensor based on thick-film hydrogen peroxide electrode system , 1999 .

[7]  Jordi Bartrolí,et al.  Polyurethane–acrylate photocurable polymeric membrane for ion-sensitive field-effect transistor based urea biosensors , 1999 .

[8]  N. Jaffrezic‐Renault,et al.  A miniaturized urea sensor based on the integration of both ammonium based urea enzyme field effect transistor and a reference field effect transistor in a single chip. , 1999, Talanta.

[9]  J Wang,et al.  Amperometric biosensors for clinical and therapeutic drug monitoring: a review. , 1999, Journal of pharmaceutical and biomedical analysis.

[10]  R. Mathies,et al.  Monolithic integrated microfluidic DNA amplification and capillary electrophoresis analysis system , 2000 .

[11]  H. Arakawa,et al.  Performance Characteristics of a Urea Microsensor Employing a Micromachined Carbon Dioxide Electrode , 2000 .

[12]  H. Suzuki,et al.  Fabrication of a sensing module using micromachined biosensors. , 2001, Biosensors & bioelectronics.

[13]  E. Verpoorte.  Microfluidic chips for clinical and forensic analysis , 2002, Electrophoresis.

[14]  Gwo-Bin Lee,et al.  Micromachined polymerase chain reaction system for multiple DNA amplification of upper respiratory tract infectious diseases. , 2005, Biosensors & bioelectronics.

[15]  Hiroaki Suzuki,et al.  Integrated microfluidic system for the simultaneous determination of ammonia, creatinine, and urea , 2005 .

[16]  P. Sharma,et al.  Creatinine sensor based on a molecularly imprinted polymer-modified hanging mercury drop electrode. , 2006, Talanta.

[17]  Gwo-Bin Lee,et al.  Pneumatically driven peristaltic micropumps utilizing serpentine-shape channels , 2006 .

[18]  Gwo-Bin Lee,et al.  Integrated microfluidic systems for automatic glucose sensing and insulin injection , 2007 .

[19]  T. Chou,et al.  An electrochemical albumin-sensing system utilizing microfluidic technology , 2007 .

[20]  Gwo-Bin Lee,et al.  Membrane-activated microfluidic rotary devices for pumping and mixing , 2007, Biomedical microdevices.

[21]  Artur Dybko,et al.  Microfluidic system with electrochemical and optical detection , 2007 .

[22]  Salvador Alegret,et al.  Bioelectronic tongue for the simultaneous determination of urea, creatinine and alkaline ions in clinical samples. , 2008, Biosensors & bioelectronics.

[23]  Gwo-Bin Lee,et al.  A pneumatic micropump incorporated with a normally closed valve capable of generating a high pumping rate and a high back pressure , 2009 .

[24]  M. Syu,et al.  Ionic effect investigation of a potentiometric sensor for urea and surface morphology observation of entrapped urease/polypyrrole matrix. , 2009, Biosensors & bioelectronics.