Enhanced motility in NIH 3T3 fibroblasts that overexpress gelsolin.

Increasing the content of the actin-binding protein gelsolin in cultured mouse fibroblasts by up to 125 percent by gene transfection proportionally enhanced the rate at which the cells migrated through porous filters toward a gradient of serum and closed a wound made on a confluent monolayer of cells in a tissue culture dish. These results provide direct evidence that gelsolin, which promotes both actin assembly and disassembly in vitro, is an important element in fibroblast locomotion and demonstrate that the manipulation of intracellular machinery can increase cell motility.

[1]  T. Stossel,et al.  Gelsolin-actin interaction and actin polymerization in human neutrophils , 1990, The Journal of cell biology.

[2]  A. Füchtbauer,et al.  Differential effects of gelsolins on tissue culture cells. , 1990, Cell motility and the cytoskeleton.

[3]  T. Stossel,et al.  From signal to pseudopod. How cells control cytoplasmic actin assembly. , 1989, The Journal of biological chemistry.

[4]  P. Janmey,et al.  Identification of critical functional and regulatory domains in gelsolin , 1989, The Journal of cell biology.

[5]  P. Janmey,et al.  Gelsolin-polyphosphoinositide interaction. Full expression of gelsolin-inhibiting function by polyphosphoinositides in vesicular form and inactivation by dilution, aggregation, or masking of the inositol head group. , 1989, The Journal of biological chemistry.

[6]  J. Segall,et al.  A Dictyostelium mutant deficient in severin, an F-actin fragmenting protein, shows normal motility and chemotaxis , 1989, The Journal of cell biology.

[7]  J. Hartwig,et al.  Association of gelsolin with actin filaments and cell membranes of macrophages and platelets , 1989, The Journal of cell biology.

[8]  S. J. Smith,et al.  Actions of cytochalasins on the organization of actin filaments and microtubules in a neuronal growth cone , 1988, The Journal of cell biology.

[9]  D. Kwiatkowski,et al.  Predominant induction of gelsolin and actin-binding protein during myeloid differentiation. , 1988, The Journal of biological chemistry.

[10]  W. Loomis,et al.  Cell motility and chemotaxis in Dictyostelium amebae lacking myosin heavy chain. , 1988, Developmental biology.

[11]  J. White,et al.  Cortical flow in animal cells. , 1988, Science.

[12]  A. Noegel,et al.  Inactivation of the alpha-actinin gene in Dictyostelium. , 1988, Developmental genetics.

[13]  P. Janmey,et al.  Polyphosphoinositide micelles and polyphosphoinositide-containing vesicles dissociate endogenous gelsolin-actin complexes and promote actin assembly from the fast-growing end of actin filaments blocked by gelsolin. , 1987, The Journal of biological chemistry.

[14]  P. Janmey,et al.  Reversible binding of actin to gelsolin and profilin in human platelet extracts , 1987, The Journal of cell biology.

[15]  L. Kedes,et al.  A human beta-actin expression vector system directs high-level accumulation of antisense transcripts. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[16]  D A Knecht,et al.  Antisense RNA inactivation of myosin heavy chain gene expression in Dictyostelium discoideum. , 1987, Science.

[17]  J. Spudich,et al.  Disruption of the Dictyostelium myosin heavy chain gene by homologous recombination. , 1987, Science.

[18]  Jonathan A. Cooper,et al.  Microinjection of gelsolin into living cells , 1987, The Journal of cell biology.

[19]  P. Janmey,et al.  Modulation of gelsolin function by phosphatidylinositol 4,5-bisphosphate , 1987, Nature.

[20]  S. Orkin,et al.  Plasma and cytoplasmic gelsolins are encoded by a single gene and contain a duplicated actin-binding domain , 1986, Nature.

[21]  J. Hartwig,et al.  Ca2+ control of actin filament length. Effects of macrophage gelsolin on actin polymerization. , 1981, The Journal of biological chemistry.

[22]  K. Zaner,et al.  Ca2+ control of actin gelation. Interaction of gelsolin with actin filaments and regulation of actin gelation. , 1980, The Journal of biological chemistry.

[23]  H. Towbin,et al.  Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[24]  M. M. Bradford.  A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. , 1976, Analytical biochemistry.