The effects of storage and development on Agaricus bisporus proteases

SummaryChanges in protein level and protease activity were compared in two tissues of the sporophore of Agaricus bisporus. The skin tissue (pilei pellis) contains on average 58% more protein and 88% more protease activity on a fresh-weight basis than the flesh tissue (pileus trama) of freshly harvested sporophores. The protein level of the tissue fell as the mushrooms grew and developed while in contact with their mycelium, but the protease activities showed no correlated trend. A large increase in proteolytic activity and a decrease in protein content was observed in the tissues of harvested mushrooms after 5 days’ storage at 18°C. The results are discussed in relation to mushroom quality during storage and the development of mushrooms before and after harvesting.

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