Multilayered Scaffold with a Compact Interfacial Layer Enhances Osteochondral Defect Repair.

Repairing osteochondral defect (OCD) using advanced biomaterials that structurally, biologically, and mechanically fulfill the criteria for stratified tissue regeneration remains a significant challenge for researchers. Here, a multilayered scaffold (MLS) with hierarchical organization and heterogeneous composition is developed to mimic the stratified structure and complex components of natural osteochondral tissues. Specifically, the intermediate compact interfacial layer within the MLS is designed to resemble the osteochondral interface to realize the closely integrated layered structure. Subsequently, macroscopic observations, histological evaluation, and biomechanical and biochemical assessments are performed to evaluate the ability of the MLS of repairing OCD in a goat model. By 48 weeks postimplantation, superior hyalinelike cartilage and sound subchondral bone are observed in the MLS group. Furthermore, the biomimetic MLS significantly enhances the biomechanical and biochemical properties of the neo-osteochondral tissue. Taken together, these results confirm the potential of this optimized MLS as an advanced strategy for OCD repair.

[1]  Jun Ma,et al.  Selective laser sintering scaffold with hierarchical architecture and gradient composition for osteochondral repair in rabbits. , 2017, Biomaterials.

[2]  Ali Khademhosseini,et al.  Cell-laden hydrogels for osteochondral and cartilage tissue engineering. , 2017, Acta biomaterialia.

[3]  Yunfeng Lin,et al.  The fabrication of biomimetic biphasic CAN-PAC hydrogel with a seamless interfacial layer applied in osteochondral defect repair , 2017, Bone Research.

[4]  Xin Fu,et al.  Structurally and Functionally Optimized Silk‐Fibroin–Gelatin Scaffold Using 3D Printing to Repair Cartilage Injury In Vitro and In Vivo , 2017, Advanced materials.

[5]  Jian Liu,et al.  Biomimetic design and fabrication of multilayered osteochondral scaffolds by low-temperature deposition manufacturing and thermal-induced phase-separation techniques , 2017, Biofabrication.

[6]  Yingqian Wang,et al.  A Difunctional Regeneration Scaffold for Knee Repair based on Aptamer‐Directed Cell Recruitment , 2017, Advanced materials.

[7]  B. Mandal,et al.  Mimicking Hierarchical Complexity of the Osteochondral Interface Using Electrospun Silk-Bioactive Glass Composites. , 2017, ACS applied materials & interfaces.

[8]  Dongan Wang,et al.  Cell-Free HA-MA/PLGA Scaffolds with Radially Oriented Pores for In Situ Inductive Regeneration of Full Thickness Cartilage Defects. , 2016, Macromolecular bioscience.

[9]  Xin Zhang,et al.  Transplantation of allogenic chondrocytes with chitosan hydrogel-demineralized bone matrix hybrid scaffold to repair rabbit cartilage injury. , 2016, Biomaterials.

[10]  Farshid Guilak,et al.  Fabrication of anatomically-shaped cartilage constructs using decellularized cartilage-derived matrix scaffolds. , 2016, Biomaterials.

[11]  F. O'Brien,et al.  Cell-free multi-layered collagen-based scaffolds demonstrate layer specific regeneration of functional osteochondral tissue in caprine joints. , 2016, Biomaterials.

[12]  Q. Guo,et al.  Induction of mesenchymal stem cell chondrogenic differentiation and functional cartilage microtissue formation for in vivo cartilage regeneration by cartilage extracellular matrix-derived particles. , 2016, Acta biomaterialia.

[13]  Fergal J O'Brien,et al.  Multi-layered collagen-based scaffolds for osteochondral defect repair in rabbits. , 2016, Acta biomaterialia.

[14]  Jian Liu,et al.  In Vivo Evaluation of a Novel Oriented Scaffold-BMSC Construct for Enhancing Full-Thickness Articular Cartilage Repair in a Rabbit Model , 2015, PloS one.

[15]  M. Shie,et al.  Positive effects of cell-free porous PLGA implants and early loading exercise on hyaline cartilage regeneration in rabbits. , 2015, Acta biomaterialia.

[16]  D. Robinson,et al.  Osteochondral regeneration with a novel aragonite-hyaluronate biphasic scaffold: up to 12-month follow-up study in a goat model , 2015, Journal of Orthopaedic Surgery and Research.

[17]  Jiang Peng,et al.  Phytomolecule icaritin incorporated PLGA/TCP scaffold for steroid-associated osteonecrosis: Proof-of-concept for prevention of hip joint collapse in bipedal emus and mechanistic study in quadrupedal rabbits , 2015, Biomaterials.

[18]  R. Reis,et al.  Bilayered silk/silk-nanoCaP scaffolds for osteochondral tissue engineering: In vitro and in vivo assessment of biological performance. , 2015, Acta biomaterialia.

[19]  David Dean,et al.  Evaluating 3D‐Printed Biomaterials as Scaffolds for Vascularized Bone Tissue Engineering , 2015, Advanced materials.

[20]  Shouan Zhu,et al.  Radially oriented collagen scaffold with SDF-1 promotes osteochondral repair by facilitating cell homing. , 2015, Biomaterials.

[21]  Qiang Yang,et al.  Integrated trilayered silk fibroin scaffold for osteochondral differentiation of adipose-derived stem cells. , 2014, ACS applied materials & interfaces.

[22]  Zhongmin Jin,et al.  Cartilage Repair and Subchondral Bone Migration Using 3D Printing Osteochondral Composites: A One-Year-Period Study in Rabbit Trochlea , 2014, BioMed research international.

[23]  Fergal J O'Brien,et al.  A biomimetic multi-layered collagen-based scaffold for osteochondral repair. , 2014, Acta biomaterialia.

[24]  Syam P Nukavarapu,et al.  Osteochondral tissue engineering: current strategies and challenges. , 2013, Biotechnology advances.

[25]  H. Ouyang,et al.  Bi-layer collagen/microporous electrospun nanofiber scaffold improves the osteochondral regeneration. , 2013, Acta biomaterialia.

[26]  Xuesi Chen,et al.  Repair of an articular cartilage defect using adipose-derived stem cells loaded on a polyelectrolyte complex scaffold based on poly(l-glutamic acid) and chitosan. , 2013, Acta biomaterialia.

[27]  F. Guilak,et al.  The effects of crosslinking of scaffolds engineered from cartilage ECM on the chondrogenic differentiation of MSCs. , 2013, Biomaterials.

[28]  Yongnian Yan,et al.  Bony defect repair in rabbit using hybrid rapid prototyping polylactic-co-glycolic acid/β-tricalciumphosphate collagen I/apatite scaffold and bone marrow mesenchymal stem cells , 2013, Indian journal of orthopaedics.

[29]  O. Gauthier,et al.  Effects of In Vitro Low Oxygen Tension Preconditioning of Adipose Stromal Cells on Their In Vivo Chondrogenic Potential: Application in Cartilage Tissue Repair , 2013, PloS one.

[30]  H. Ouyang,et al.  The use of type 1 collagen scaffold containing stromal cell-derived factor-1 to create a matrix environment conducive to partial-thickness cartilage defects repair. , 2013, Biomaterials.

[31]  L. Dürselen,et al.  Decellularized cartilage matrix as a novel biomatrix for cartilage tissue-engineering applications. , 2012, Tissue engineering. Part A.

[32]  Jian Liu,et al.  Oriented cartilage extracellular matrix-derived scaffold for cartilage tissue engineering. , 2012, Journal of bioscience and bioengineering.

[33]  Ali Cinar,et al.  An agent-based model for the investigation of neovascularization within porous scaffolds. , 2011, Tissue engineering. Part A.

[34]  Arndt F Schilling,et al.  High amplitude direct compressive strain enhances mechanical properties of scaffold-free tissue-engineered cartilage. , 2011, Tissue engineering. Part A.

[35]  Shuyun Liu,et al.  Fabrication and cell affinity of biomimetic structured PLGA/articular cartilage ECM composite scaffold , 2011, Journal of materials science. Materials in medicine.

[36]  Masato Sato,et al.  Intravenous administration of anti-vascular endothelial growth factor humanized monoclonal antibody bevacizumab improves articular cartilage repair , 2010, Arthritis research & therapy.

[37]  Guoping Chen,et al.  The influence of structural design of PLGA/collagen hybrid scaffolds in cartilage tissue engineering. , 2010, Biomaterials.

[38]  Akihiko Kusanagi,et al.  In vitro generation of mechanically functional cartilage grafts based on adult human stem cells and 3D-woven poly(epsilon-caprolactone) scaffolds. , 2010, Biomaterials.

[39]  David Dean,et al.  Effect of initial cell seeding density on early osteogenic signal expression of rat bone marrow stromal cells cultured on cross-linked poly(propylene fumarate) disks. , 2009, Biomacromolecules.

[40]  Yilin Cao,et al.  Repair of articular cartilage defect in non-weight bearing areas using adipose derived stem cells loaded polyglycolic acid mesh. , 2009, Biomaterials.

[41]  T. O’Shea,et al.  Bilayered scaffolds for osteochondral tissue engineering. , 2008, Tissue engineering. Part B, Reviews.

[42]  Jiang Peng,et al.  A cartilage ECM-derived 3-D porous acellular matrix scaffold for in vivo cartilage tissue engineering with PKH26-labeled chondrogenic bone marrow-derived mesenchymal stem cells. , 2008, Biomaterials.

[43]  D. Saris,et al.  International Cartilage Repair Society (ICRS) and Oswestry macroscopic cartilage evaluation scores validated for use in Autologous Chondrocyte Implantation (ACI) and microfracture. , 2007, Osteoarthritis and cartilage.

[44]  R. Johnson,et al.  HIF1α regulation of Sox9 is necessary to maintain differentiation of hypoxic prechondrogenic cells during early skeletogenesis , 2007, Development.

[45]  J. Urban,et al.  Factors influencing the oxygen concentration gradient from the synovial surface of articular cartilage to the cartilage-bone interface: a modeling study. , 2004, Arthritis and rheumatism.

[46]  R. Salter,et al.  The chondrogenic potential of free autogenous periosteal grafts for biological resurfacing of major full-thickness defects in joint surfaces under the influence of continuous passive motion. An experimental investigation in the rabbit. , 1986, The Journal of bone and joint surgery. American volume.