Mitochondrial genetic differentiation and morphological difference of Miniopterus fuliginosus and Miniopterus magnater in China and Vietnam

Because of its complicated systematics, the bent-winged bat is one of the most frequently studied bat species groups. In China, two morphologically similar bent-winged bat species, Miniopterus fuliginosus and Miniopterus magnater were identified, but their distribution range and genetic differentiation are largely unexplored. In this study, we applied DNA bar codes and two other mitochondrial DNA genes including morphological parameters to determine the phylogeny, genetic differentiation, spatial distribution, and morphological difference of the M. fuliginosus and M. magnater sampled from China and one site in Vietnam. Mitochondrial DNA gene genealogies revealed two monophyletic lineages throughout the Tropic of Cancer. According to DNA bar code divergences, one is M. fuliginosus corresponding to the Chinese mainland and the other is M. magnater corresponding to tropical regions including Hainan and Guangdong provinces of China and Vietnam. Their most recent common ancestor was dated to the early stage of the Quaternary glacial period (ca. 2.26 million years ago [Ma] on the basis of D-loop data, and ca. 1.69–2.37 Ma according to ND2). A population expansion event was inferred for populations of M. fuliginosus at 0.14 Ma. The two species probably arose in separate Pleistocene refugia under different climate zones. They significantly differed in forearm length, maxillary third molar width, and greatest length of the skull.

[1]  S. Puechmaille,et al.  A new species of the Miniopterus schreibersii species complex (Chiroptera: Miniopteridae) from the Maghreb Region, North Africa. , 2014, Zootaxa.

[2]  L. Christidis,et al.  Insights into the Evolution of a Cryptic Radiation of Bats: Dispersal and Ecological Radiation of Malagasy Miniopterus (Chiroptera: Miniopteridae) , 2014, PloS one.

[3]  Tinglei Jiang,et al.  Phylogeography of Hipposideros armiger (Chiroptera: Hipposideridae) in the Oriental Region: the contribution of multiple Pleistocene glacial refugia and intrinsic factors to contemporary population genetic structure , 2014 .

[4]  Koichiro Tamura,et al.  MEGA6: Molecular Evolutionary Genetics Analysis version 6.0. , 2013, Molecular biology and evolution.

[5]  J. Sumner,et al.  Mountain barriers and river conduits: phylogeographical structure in a large, mobile lizard (Varanidae: Varanus varius) from eastern Australia , 2013 .

[6]  Syntopic Occurrence in Turkey Supports Separate Species Status for Miniopterus schreibersii schreibersii and M. schreibersii pallidus (Mammalia: Chiroptera) , 2012 .

[7]  N. Baeshen,et al.  Biological Identifications Through DNA Barcodes , 2012 .

[8]  Li-biao Zhang,et al.  [Dietary composition, echolocation pulses and morphological measurements of the long-fingered bat Miniopterus fuliginosus (Chiroptera: Vespertilioninae)]. , 2011, Dong wu xue yan jiu = Zoological research.

[9]  S. Rossiter,et al.  Identifying the effects of the Pleistocene on the greater horseshoe bat, Rhinolophus ferrumequinum, in East Asia using ecological niche modelling and phylogenetic analyses , 2011 .

[10]  N. Ivanova,et al.  The Role of DNA Barcodes in Understanding and Conservation of Mammal Diversity in Southeast Asia , 2010, PloS one.

[11]  S. Berquist,et al.  Phylogeography and population genetic structure of the great leaf-nosed bat (Hipposideros armiger) in China. , 2010, The Journal of heredity.

[12]  A. Furman,et al.  Cryptic diversity of the bent-wing bat, Miniopterus schreibersii (Chiroptera: Vespertilionidae), in Asia Minor , 2010, BMC Evolutionary Biology.

[13]  Andrew T. Smith,et al.  中国兽类野外手册 = A guide to the mammals of China , 2010 .

[14]  P. Salgueiro,et al.  Population structure of a cave-dwelling bat, Miniopterus schreibersii: does it reflect history and social organization? , 2009, The Journal of heredity.

[15]  L. Christidis,et al.  The use of molecular and morphological characters to resolve the taxonomic identity of cryptic species: the case of Miniopterus manavi (Chiroptera, Miniopteridae) , 2009 .

[16]  A. Karataş,et al.  Molecular ecology and phylogeography of the bent‐wing bat complex (Miniopterus schreibersii) (Chiroptera: Vespertilionidae) in Asia Minor and adjacent regions , 2009 .

[17]  P. Hebert,et al.  Probing Evolutionary Patterns in Neotropical Birds through DNA Barcodes , 2009, PloS one.

[18]  Gang Li,et al.  Phylogeography of the greater horseshoe bat, Rhinolophus ferrumequinum: contrasting results from mitochondrial and microsatellite data , 2009, Molecular ecology.

[19]  J. Morales,et al.  Regionally and climatically restricted patterns of distribution of genetic diversity in a migratory bat species, Miniopterus schreibersii (Chiroptera: Vespertilionidae) , 2008, BMC Evolutionary Biology.

[20]  C. Miller-Butterworth,et al.  Isolation and characterization of microsatellite loci in the long‐fingered bat Miniopterus fuliginosus , 2008, Molecular ecology resources.

[21]  R. Hanner,et al.  DNA barcoding in surveys of small mammal communities: a field study in Suriname , 2008, Molecular ecology resources.

[22]  A. Rambaut,et al.  BEAST: Bayesian evolutionary analysis by sampling trees , 2007, BMC Evolutionary Biology.

[23]  L. Christidis,et al.  Specific Status of Populations on Madagascar Referred to Miniopterus fraterculus (Chiroptera: Vespertilionidae), with Description of a New Species , 2007 .

[24]  Nai-fa Liu,et al.  Phylogeography of rusty-necklaced partridge (Alectoris magna) in northwestern China. , 2007, Molecular phylogenetics and evolution.

[25]  Hu Chi-ming,et al.  Phylogeographic structure of Primula obconica (Primulaceae) inferred from chloroplast microsatellites (cpSSRs) markers , 2007 .

[26]  Jian-jun Gao,et al.  Genus Amiota Loew (Diptera: Drosophilidae) from the Qinling mountain system, central China , 2007 .

[27]  T. Saitoh,et al.  Role of Tannin-Binding Salivary Proteins and Tannase-Producing Bacteria in the Acclimation of the Japanese Wood Mouse to Acorn Tannins , 2006, Journal of Chemical Ecology.

[28]  Olivier Gascuel,et al.  PHYML Online: A Web Server for Fast Maximum Likelihood-Based Phylogenetic Inference , 2018 .

[29]  Eliot Marshall,et al.  Will DNA Bar Codes Breathe Life Into Classification? , 2005, Science.

[30]  Michael P. Cummings,et al.  PAUP* [Phylogenetic Analysis Using Parsimony (and Other Methods)] , 2004 .

[31]  J. A. Mckenzie,et al.  Molecular systematics and biogeography of the bent-wing bat complex Miniopterus schreibersii (Kuhl, 1817) (Chiroptera: Vespertilionidae). , 2004, Molecular phylogenetics and evolution.

[32]  Shuyi Zhang,et al.  Molecular studies on the classification of Miniopterus schreibersii (Chiroptera: Vespertilionidae) inferred from mitochondrial cytochrome b sequences , 2004 .

[33]  Xavier Messeguer,et al.  DnaSP, DNA polymorphism analyses by the coalescent and other methods , 2003, Bioinform..

[34]  E. Harley,et al.  Strong population substructure is correlated with morphology and ecology in a migratory bat , 2003, Nature.

[35]  R. Nikula,et al.  Phylogeography of Cerastoderma glaucum (Bivalvia: Cardiidae) across Europe: a major break in the Eastern Mediterranean , 2003 .

[36]  D. Swofford.  PAUP*: Phylogenetic analysis using parsimony (*and other methods), Version 4.0b10 , 2002 .

[37]  G. Hewitt.  The genetic legacy of the Quaternary ice ages , 2000, Nature.

[38]  L. Excoffier,et al.  NO EVIDENCE OF BOTTLENECK IN THE POSTGLACIAL RECOLONIZATION OF EUROPE BY THE NOCTULE BAT (NYCTALUS NOCTULA) , 1999, Evolution; international journal of organic evolution.

[39]  L. Excoffier,et al.  Estimation of past demographic parameters from the distribution of pairwise differences when the mutation rates vary among sites: application to human mitochondrial DNA. , 1999, Genetics.

[40]  N. Jablonski,et al.  Environmental change during the Quaternary in East Asia and its consequences for mammals , 1999 .

[41]  P. Taberlet,et al.  Comparative phylogeography and postglacial colonization routes in Europe , 1998, Molecular ecology.

[42]  J. Thompson,et al.  The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. , 1997, Nucleic acids research.

[43]  Y. Fu,et al.  Statistical tests of neutrality of mutations against population growth, hitchhiking and background selection. , 1997, Genetics.

[44]  W. Li,et al.  Statistical tests of neutrality of mutations. , 1993, Genetics.

[45]  H. Harpending,et al.  Population growth makes waves in the distribution of pairwise genetic differences. , 1992, Molecular biology and evolution.

[46]  G. Wilkinson,et al.  Length and sequence variation in evening bat D-loop mtDNA. , 1991, Genetics.

[47]  M. Raymo,et al.  Northern Hemisphere climate regimes during the past 3 Ma: possible tectonic connections , 1988 .

[48]  前田 喜四雄.  Studies on the classification of miniopterus in Eurasia, Australia and Melanesia , 1982 .

[49]  J. Honacki,et al.  Mammal species of the world : a taxonomic and geographic reference , 1982 .