Deployment of low-level ozone-enrichment for the preservation of chilled fresh produce

Abstract Tomatoes, strawberries, table grapes and plums were inoculated with Botrytis cinerea (grey mould), transferred to chilled storage (13 °C) and exposed to ‘clean air’ or low-level ozone-enrichment (0.1 μmol mol−1). Ozone-enrichment resulted in a substantial decline in spore production as well as visible lesion development in all treated fruit. Exposure-response studies performed specifically on tomato fruit (exposed to concentrations ranging between 0.005 and 5.0 μmol mol−1 ozone) revealed lesion development and spore production/viability to be markedly reduced in produce exposed to ozone prior to, or following, infection with B. cinerea; higher concentrations/duration of exposure yielding greater reductions in lesion development and spore production/viability. Impacts on Botrytis colonies grown on Potato Dextrose Agar (PDA) for 5–6 days at 13 °C and 95% relative humidity (RH) revealed less effects than studies on fruit inoculated with the pathogen in vivo. Taken as a whole, the results imply that ozone-induced suppression of pathogen development is due, to some extent, to impacts on fruit–pathogen interactions. This work suggests that ozone may constitute a desirable and effective residue-free alternative to traditional postharvest fungicide practices. Data presented illustrate that optimal ozone treatment regimes are likely to be commodity-specific and require detailed investigation before such practices can be contemplated commercially.

[1]  J. Visser,et al.  The endopolygalacturonase gene Bcpg1 is required for full virulence of Botrytis cinerea. , 1998, Molecular plant-microbe interactions : MPMI.

[2]  F. Geering Ozone Applications The State-of-the-Art in Switzerland , 1999 .

[3]  M. Schirra Advances in postharvest diseases and disorders control of citrus fruit. , 1999 .

[4]  C. Krause,et al.  Effects of Ozone on the Sporulation, Germination, and Pathogenicity of Botrytis cinerea , 1978 .

[5]  R. Prange,et al.  Effect of ozone and storage temperature on postharvest diseases and physiology of carrots (Daucus carota L) , 1994 .

[6]  R. C. Weast CRC Handbook of Chemistry and Physics , 1973 .

[7]  R. Ben-arie,et al.  Ozone for control of post-harvest decay of table grapes caused byRhizopus stolonifer , 1996 .

[8]  J. Smilanick,et al.  Ozone gas penetration and control of the sporulation of Penicillium digitatum and Penicillium italicum within commercial packages of oranges during cold storage , 2003 .

[9]  J. Ogawa,et al.  THE CHEMICAL CONTROL OF POSTHARVEST DISEASES: Deciduous Fruits, Berries, Vegetables and Root/Tuber Crops , 1988 .

[10]  I. Singleton,et al.  Impact of atmospheric ozone-enrichment on quality-related attributes of tomato fruit , 2007 .

[11]  Jun Song,et al.  DO NEGATIVE AIR IONS REDUCE DECAY OF FRESH FRUITS AND VEGETABLES , 2001 .

[12]  Rip G. Rice,et al.  Ozone in the United States of America -- State-Of-The-Art , 1999 .

[13]  A. Snowdon A colour atlas of post-harvest diseases and disorders of fruits and vegetables. Volume 1: General introduction and fruits. , 1990 .

[14]  Joseph L. Smilanick,et al.  Effects of continuous 0.3 ppm ozone exposure on decay development and physiological responses of peaches and table grapes in cold storage , 2002 .

[15]  F. Payne,et al.  Ozone Storage Effects on Anthocyanin Content and Fungal Growth in Blackberries , 1995 .

[16]  P. Schreier,et al.  An ozone-responsive region of the grapevine resveratrol synthase promoter differs from the basal pathogen-responsive sequence , 1997, Plant Molecular Biology.

[17]  S. Foster,et al.  Ascorbate Oxidase-Dependent Changes in the Redox State of the Apoplast Modulate Gene Transcript Accumulation Leading to Modified Hormone Signaling and Orchestration of Defense Processes in Tobacco1[W] , 2006, Plant Physiology.

[18]  Y. Elad,et al.  Multiple fungicide resistance to benzimidazoles, dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel , 1992 .

[19]  A. G. Pérez,et al.  Effects of ozone treatment on postharvest strawberry quality. , 1999, Journal of agricultural and food chemistry.

[20]  D. H. Spalding Effects of ozone atmospheres on spoilage of fruits and vegetables after harvest , 1968 .

[21]  M. Khan,et al.  Effects of intermittent ozone exposures on powdery mildew of cucumber , 1999 .

[22]  Rip G. Rice,et al.  Century 21 - Pregnant with Ozone , 2002 .

[23]  S. Droby,et al.  Commercial Testing of Aspire: A Yeast Preparation for the Biological Control of Postharvest Decay of Citrus , 1998 .

[24]  J. Smilanick,et al.  Effect of Gaseous Ozone Exposure on the Development of Green and Blue Molds on Cold Stored Citrus Fruit. , 2001, Plant disease.

[25]  J. Smilanick,et al.  Impact of Ozonated Water on the Quality and Shelf-life of Fresh Citrus Fruit, Stone Fruit, and Table Grapes , 2002 .

[26]  D. M. Graham,et al.  Use of ozone for food processing , 1997 .

[27]  V. Escalona,et al.  Effect of cyclic exposure to ozone gas on physicochemical, sensorial and microbial quality of whole and sliced tomatoes , 2006 .

[28]  G. Stotzky,et al.  Effects of ozone on the germination of fungus spores. , 1969, Canadian journal of microbiology.

[29]  D. Slaughter,et al.  A Fluorescent Lectin Test for Mold in Raw Tomato Juice , 2000 .

[30]  Joseph L. Smilanick,et al.  USE OF OZONE IN STORAGE AND PACKING FACILITIES , 2003 .

[31]  G. Chastagner A Fungicide-Wax Treatment to Suppress Botrytis cinerea and Protect Fresh-Market Tomatoes , 1979 .

[32]  A. Snowdon General introduction and fruits , 1990 .

[33]  R. Spotts,et al.  Effect of Ozonated Water on Postharvest Pathogens of Pear in Laboratory and Packinghouse Tests , 1992 .

[34]  C. Pieterse,et al.  Priming in plant-pathogen interactions. , 2002, Trends in plant science.