论文信息 - Adaptive optics light-sheet microscopy for functional neuroimaging - 字舞流文

Adaptive optics light-sheet microscopy for functional neuroimaging

We present a new implementation of adaptive optics for light-sheet microscopy, with a direct extended-scene wavefront sensing measurement for fast aberration correction. We report AO-enhanced images of GCaMP in freshly dissected drosophila brains.

Xavier Levecq | Alexandra Fragola | Vincent Loriette | Fabrice Harms | Georges Farkouh | Antoine Hubert | Cynthia Veilly | Sophie Imperato | Francois Rouyer

[1] Misha B. Ahrens,et al. Visualizing Whole-Brain Activity and Development at the Single-Cell Level Using Light-Sheet Microscopy , 2015, Neuron.

[2] J. J. Macklin,et al. High-performance calcium sensors for imaging activity in neuronal populations and microcompartments , 2019, Nature Methods.

[3] Kevin M. Dean,et al. Light-Sheet Microscopy of Cleared Tissues with Isotropic, Subcellular Resolution , 2019, Nature Methods.

[4] Na Ji. Adaptive optical fluorescence microscopy , 2017, Nature Methods.

[5] Xavier Levecq,et al. Adaptive optics light-sheet microscopy based on direct wavefront sensing without any guide star. , 2019, Optics letters.

[6] Jonathan M. Taylor,et al. 3D adaptive optics in a light sheet microscope. , 2012, Optics express.

[7] Martin J. Booth,et al. Adaptive optical microscopy: the ongoing quest for a perfect image , 2014, Light: Science & Applications.

[8] Elliot M. Meyerowitz,et al. Observing the cell in its native state: Imaging subcellular dynamics in multicellular organisms , 2018, Science.

[9] Jordi Andilla,et al. Deep and Clear Optical Imaging of Thick Inhomogeneous Samples , 2012, PloS one.

[10] Stefan R. Pulver,et al. Ultra-sensitive fluorescent proteins for imaging neuronal activity , 2013, Nature.