Wide-field, full-field optical coherence microscopy for high-axial-resolution phase and amplitude imaging.

An original single-objective, full-field optical coherence microscopy system is reported that is capable of imaging both the phase and the amplitude of semi-transparent samples over a field of view of 17.5  mm×17.5  mm with an axial sectioning resolution of 1.5 μm. A special stack acquisition arrangement ensures optimal reachable imaging depth. Several phase-shifting interferometry algorithms for phase measurement with broadband light are compared theoretically and experimentally. Using the phase information, noninvasive depth-resolved topographic images of multilayer samples are produced to characterize each layer by measuring their defects and curvature with a nanometric scale precision. Using the amplitude information, tomographic images with a constant detection sensitivity of ∼80  dB through the entire field of view are obtained and applied to biological specimens.

[1]  A. Fercher,et al.  In vivo human retinal imaging by Fourier domain optical coherence tomography. , 2002, Journal of biomedical optics.

[2]  R P Salathé,et al.  Spatially incoherent illumination as a mechanism for cross-talk suppression in wide-field optical coherence tomography. , 2004, Optics letters.

[3]  Kieran G. Larkin,et al.  Efficient nonlinear algorithm for envelope detection in white light interferometry , 1996 .

[4]  J. Schuman,et al.  Optical coherence tomography. , 2000, Science.

[5]  Kai Wu,et al.  Multilayer thin-film inspection through measurements of reflection coefficients. , 2011, Optics letters.

[6]  M. Miwa,et al.  Low-coherent quantitative phase microscope for nanometer-scale measurement of living cells morphology. , 2008, Optics express.

[7]  A C Boccara,et al.  Sinusoidally phase-modulated interference microscope for high-speed high-resolution topographic imagery. , 2001, Optics letters.

[8]  Claude Boccara,et al.  Thermal-light full-field optical coherence tomography in the 1.2 μm wavelength region , 2006 .

[9]  Adolf Friedrich Fercher.  Optical coherence tomography. , 1996 .

[10]  A. Dubois,et al.  Phase-map measurements by interferometry with sinusoidal phase modulation and four integrating buckets. , 2001, Journal of the Optical Society of America. A, Optics, image science, and vision.

[11]  Yuuki Watanabe,et al.  Three-dimensional wide-field optical coherence tomography using an ultrahigh-speed CMOS camera , 2008 .

[12]  Harald Sattmann,et al.  En face imaging of single cell layers by differential phase-contrast optical coherence microscopy. , 2002, Optics letters.

[13]  Joseph A Izatt,et al.  Investigating nanoscale cellular dynamics with cross-sectional spectral domain phase microscopy. , 2007, Optics express.

[14]  Katharine Grieve,et al.  Full-field optical coherence microscopy , 2004, Advanced Laser Technologies.

[15]  J. Wyant,et al.  Improved vertical-scanning interferometry. , 2000, Applied optics.

[16]  C. Boccara,et al.  Ultrahigh-resolution full-field optical coherence tomography. , 2004, Applied optics.

[17]  James C. Wyant,et al.  Surface Profilers, Multiple Wavelength, and White Light Intereferometry , 2006 .

[18]  J. Fujimoto,et al.  Optical coherence microscopy in scattering media. , 1994, Optics letters.

[19]  A. Boccara,et al.  Defocus test and defocus correction in full-field optical coherence tomography. , 2009, Optics letters.

[20]  Chandra Shakher,et al.  High-resolution full-field optical coherence microscopy using a Mirau interferometer for the quantitative imaging of biological cells. , 2011, Applied optics.

[21]  Antonello De Martino,et al.  Full-field optical coherence tomography with thermal light. , 2002, Applied optics.

[22]  Leslie L. Deck,et al.  Surface Profiling by Analysis of White-light Interferograms in the Spatial Frequency Domain , 1995 .

[23]  K. Creath.  V Phase-Measurement Interferometry Techniques , 1988 .

[24]  Antoine Federici,et al.  Three-band, 1.9-μm axial resolution full-field optical coherence microscopy over a 530-1700 nm wavelength range using a single camera. , 2014, Optics letters.

[25]  C. Werner,et al.  Satellite radar interferometry: Two-dimensional phase unwrapping , 1988 .

[26]  A. Boccara,et al.  Thermal-light full-field optical coherence tomography. , 2002, Optics letters.

[27]  Shoude Chang,et al.  Large Area Full-Field Optical Coherence Tomography and its Applications , 2008 .

[28]  J G Fujimoto,et al.  High-resolution optical coherence microscopy for high-speed, in vivo cellular imaging. , 2003, Optics letters.

[29]  M S Feld,et al.  Phase-dispersion optical tomography. , 2001, Optics letters.

[30]  Soohyun Kim,et al.  Measurement of the thickness profile of a transparent thin film deposited upon a pattern structure with an acousto-optic tunablefilter. , 2002, Optics letters.

[31]  C. Fang-Yen,et al.  Tomographic phase microscopy , 2008, Nature Methods.

[32]  J. Biegen,et al.  Determination of the phase change on reflection from two-beam interference. , 1994, Optics letters.

[33]  N Tsurumachi,et al.  Wide-field optical coherence tomography: imaging of biological tissues. , 2002, Applied optics.

[34]  Chen D. Lu,et al.  Retinal, anterior segment and full eye imaging using ultrahigh speed swept source OCT with vertical-cavity surface emitting lasers , 2012, Biomedical optics express.

[35]  Freddy T. Nguyen,et al.  Optical coherence tomography: a review of clinical development from bench to bedside. , 2007, Journal of biomedical optics.

[36]  T. Eiju,et al.  Digital phase-shifting interferometry: a simple error-compensating phase calculation algorithm. , 1987, Applied optics.

[37]  Justin Pedro,et al.  Multidimensional en-face OCT imaging of the retina. , 2009, Optics express.

[38]  P. Marquet,et al.  Marker-free phase nanoscopy , 2013, Nature Photonics.

[39]  Taner Akkin,et al.  Quantitative phase-contrast imaging of cells with phase-sensitive optical coherence microscopy. , 2004, Optics letters.

[40]  Lingfeng Yu,et al.  Full-color three-dimensional microscopy by wide-field optical coherence tomography. , 2004, Optics express.

[41]  J. Fujimoto,et al.  High-resolution line-scanning optical coherence microscopy. , 2007, Optics letters.

[42]  A. Boccara,et al.  High-resolution full-field optical coherence tomography with a Linnik microscope. , 2002, Applied optics.

[43]  Serge Monneret,et al.  Tomographic incoherent phase imaging, a diffraction tomography alternative for any white-light microscope , 2013, Photonics West - Biomedical Optics.

[44]  A. Fercher,et al.  Quantitative differential phase measurement and imaging in transparent and turbid media by optical coherence tomography. , 2001, Optics letters.

[45]  H Saint-Jalmes,et al.  Full-field optical coherence microscopy. , 1998, Optics letters.

[46]  S. W. Kim,et al.  Thickness-profile measurement of transparent thin-film layers by white-light scanning interferometry. , 1999, Applied optics.