Novel electrochemical immune sensor based on Hep-PGA-PPy nanoparticles for detection of α-Fetoprotein in whole blood.

A simple and accurate immune sensor for quantitative detection of α-Fetoprotein (AFP) was developed based on the immobilization of antigen on the surface of Hep-PGA-PPy nanoparticles modified glassy carbon electrodes (GCE). The obtained Hep-PGA-PPy nanoparticles were characterized by fourier transform infrared (FT-IR) spectra and transmission electron microscopy (TEM). And the blood compatibility of Hep-PGA-PPy nanoparticles was investigated by in vitro coagulation tests, hemolysis assay and whole blood adhesion tests. Combining the conductive property of polypyrrole (PPy) and the biocompatibility of heparin (Hep), the Hep-PGA-PPy nanoparticles could improve not only the anti-biofouling effect the electrode, but also improved the electrochemical properties of the immune sensor. Under optimal conditions, the proposed immune sensor could detect AFP in a linear range from 0.1 to 100 ng mL-1 with a detection limit of 0.099 ng mL-1 at the signal-to-noise ratio of 3, and it also possessed good reproducibility and storage stability. Furthermore, the detection of AFP in five human blood samples also showed satisfactory accuracy with low relative errors. Thus, the developed immune sensor which showed acceptable reproducibility, selectivity, stability and accuracy could be potentially used for the detection of whole blood samples directly.

[1]  Ping Liu,et al.  High-performance fluorescence-encoded magnetic microbeads as microfluidic protein chip supports for AFP detection. , 2016, Analytica chimica acta.

[2]  H. B. Lim,et al.  Metal-doped inorganic nanoparticles for multiplex detection of biomarkers by a sandwich-type ICP-MS immunoassay. , 2016, Analytica chimica acta.

[3]  Jiangna Guo,et al.  Water‐soluble cationic polypyrrole based probe for fluorometric and voltammetric detection of base pair mismatched oligonucleotides , 2015 .

[4]  Xuelian Huang,et al.  Heparin-Like Chitosan Hydrogels with Tunable Swelling Behavior, Prolonged Clotting Times, and Prevented Contact Activation and Complement Activation. , 2016, Biomacromolecules.

[5]  M. Karttunen,et al.  Mimicking the biomolecular control of calcium oxalate monohydrate crystal growth: effect of contiguous glutamic acids. , 2012, Langmuir : the ACS journal of surfaces and colloids.

[6]  Zhang Lan,et al.  High-performance polypyrrole nanoparticles counter electrode for dye-sensitized solar cells , 2008 .

[7]  Fujian Xu,et al.  Hemocompatible and antibiofouling PU-F127 nanospheres platform for application to glucose detection in whole blood. , 2013, Journal of materials chemistry. B.

[8]  Arti Vashist,et al.  Electrochemical monitoring-on-chip (E-MoC) of HIV-infection in presence of cocaine and therapeutics. , 2016, Biosensors & bioelectronics.

[9]  N. Morgan,et al.  Electrochemical immunosensors for detection of cancer protein biomarkers. , 2012, ACS nano.

[10]  M. Liu,et al.  Stabilized hemocompatible coating of nitinol devices based on photo-cross-linked alginate/heparin multilayer. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[11]  R. Yuan,et al.  Silver–graphene oxide nanocomposites as redox probes for electrochemical determination of α-1-fetoprotein , 2013 .

[12]  Xingyu Jiang,et al.  A dual-readout chemiluminescent-gold lateral flow test for multiplex and ultrasensitive detection of disease biomarkers in real samples. , 2016, Nanoscale.

[13]  Hong Dai,et al.  Signal-on electrochemiluminescent immunosensor based on poly(amidoamine) dendrimer functionalized carbon nanodots amplification for ultrasensitive detection of α-fetoprotein , 2016 .

[14]  Baoshan He.  Differential pulse voltammetric assay for the carcinoembryonic antigen using a glassy carbon electrode modified with layered molybdenum selenide, graphene, and gold nanoparticles , 2016, Microchimica Acta.

[15]  Yunhui Yang,et al.  Alpha-1-fetoprotein antibody functionalized Au nanoparticles: Catalytic labels for the electrochemical detection of α-1-fetoprotein based on TiO2 nanoparticles synthesized with ionic liquid , 2009 .

[16]  C. Egles,et al.  Polyelectrolyte multilayer films with pegylated polypeptides as a new type of anti-microbial protection for biomaterials. , 2004, Biomaterials.

[17]  Miss A.O. Penney.  (b) , 1974, The New Yale Book of Quotations.

[18]  F. Yan,et al.  Facile preparation of water dispersible polypyrrole nanotube-supported silver nanoparticles for hydrogen peroxide reduction and surface-enhanced Raman scattering , 2012 .

[19]  Guoan Zhang,et al.  Metal oxide intercalated layered double hydroxide nanosphere: With enhanced electrocatalyic activity towards H2O2 for biological applications , 2017 .

[20]  R. Nolte,et al.  Conducting polymers with confined dimensions: Track-etch membranes for amperometric biosensor applications , 2002 .

[21]  M. Ersoz,et al.  An electrochemical biosensor based on human serum albumin/graphene oxide/3-aminopropyltriethoxysilane modified ITO electrode for the enantioselective discrimination of D- and L-tryptophan. , 2013, Biosensors & bioelectronics.

[22]  李幼升,et al.  Ph , 1989 .

[23]  Rajesh,et al.  Biomolecular immobilization on conducting polymers for biosensing applications. , 2007, Biomaterials.

[24]  Paramita Karfa,et al.  A battle between spherical and cube-shaped Ag/AgCl nanoparticle modified imprinted polymer to achieve femtogram detection of alpha-feto protein. , 2016, Journal of materials chemistry. B.

[25]  Wei Wei,et al.  A label-free ultrasensitive assay of 8-hydroxy-2'-deoxyguanosine in human serum and urine samples via polyaniline deposition and tetrahedral DNA nanostructure. , 2016, Analytica chimica acta.

[26]  J. Bao,et al.  Preparation of polypropylene superhydrophobic surface and its blood compatibility. , 2010, Colloids and surfaces. B, Biointerfaces.

[27]  Jian Shen,et al.  Applications of antibiofouling PEG-coating in electrochemical biosensors for determination of glucose in whole blood , 2013 .

[28]  R. Weksberg,et al.  Serum α-fetoprotein levels in Beckwith-Wiedemann syndrome ☆ ☆☆ , 2000 .

[29]  K. Popat,et al.  Hemocompatibility of titania nanotube arrays. , 2010, Journal of biomedical materials research. Part A.

[30]  Junyong Sun,et al.  An electrochemical amperometric immunobiosensor for label-free detection of α-fetoprotein based on amine-functionalized graphene and gold nanoparticles modified carbon ionic liquid electrode , 2011 .

[31]  F. Yan,et al.  Polypyrrole nanotube-supported gold nanoparticles: An efficient electrocatalyst for oxygen reduction and catalytic reduction of 4-nitrophenol , 2012 .

[32]  Da-Ming Wang,et al.  Preparation of γ-PGA/chitosan composite tissue engineering matrices , 2005 .

[33]  A. Riskin,et al.  Alpha-fetoprotein in the early neonatal period--a large study and review of the literature. , 2004, Clinica chimica acta; international journal of clinical chemistry.

[34]  R. Huddart,et al.  The present and future of serum diagnostic tests for testicular germ cell tumours , 2016, Nature Reviews Urology.

[35]  Na Liu,et al.  An ultrasensitive amperometric immunosensor for zearalenones based on oriented antibody immobilization on a glassy carbon electrode modified with MWCNTs and AuPt nanoparticles , 2016, Microchimica Acta.

[36]  Jun Chen,et al.  Novel Nano-silicon / Polypyrrole Composites for Lithium Storage , 2007 .

[37]  M. Feuring,et al.  Dabigatran etexilate – a novel, reversible, oral direct thrombin inhibitor: Interpretation of coagulation assays and reversal of anticoagulant activity , 2010, Thrombosis and Haemostasis.

[38]  Ki Joong Lee,et al.  Enzyme-coupled nanoplasmonic biosensing of cancer markers in human serum. , 2016, Biosensors & bioelectronics.

[39]  Na Liu,et al.  Platinum porous nanoparticles hybrid with metal ions as probes for simultaneous detection of multiplex cancer biomarkers. , 2014, Biosensors & bioelectronics.

[40]  N. Huang,et al.  Tailoring of the titanium surface by immobilization of heparin/fibronectin complexes for improving blood compatibility and endothelialization: an in vitro study. , 2011, Biomacromolecules.

[41]  W. Wilson,et al.  Energetic basis for selective recognition of T*G mismatched base pairs in DNA by imidazole-rich polyamides. , 2004, Nucleic acids research.

[42]  Thomas Kissel,et al.  In vitro cytotoxicity testing of polycations: influence of polymer structure on cell viability and hemolysis. , 2003, Biomaterials.

[43]  Houbin Li,et al.  Synthesis and characterization of polypyrrole doped with anionic spherical polyelectrolyte brushes , 2012 .

[44]  Yihe Zhang,et al.  Label-free immunosensor based on Pd nanoplates for amperometric immunoassay of alpha-fetoprotein. , 2014, Biosensors & bioelectronics.

[45]  F. Yan,et al.  Fabrication of ionic liquid-functionalized polypyrrole nanotubes decorated with platinum nanoparticles and their electrocatalytic oxidation of methanol. , 2011, Chemical communications.

[46]  G. G. Stokes.  "J." , 1890, The New Yale Book of Quotations.

[47]  Ciara K O'Sullivan,et al.  Electron permeable self-assembled monolayers of dithiolated aromatic scaffolds on gold for biosensor applications. , 2008, Analytical chemistry.

[48]  Huafeng Yang,et al.  Direct electrochemistry of glucose oxidase and biosensing for glucose based on graphene. , 2009, Analytical chemistry.

[49]  J. Bao,et al.  Novel Blood-Compatible Polyurethane Ionomer Nanoparticles , 2009 .

[50]  Changming Mao,et al.  A label-free immunosensor based on modified mesoporous silica for simultaneous determination of tumor markers. , 2011, Biosensors & bioelectronics.

[51]  Min Zhou,et al.  An innovative glucose biosensor using antibiofouling Au-F127 nanospheres. , 2013, Journal of biomedical nanotechnology.

[52]  Chen Wang,et al.  Two methods for glass surface modification and their application in protein immobilization. , 2007, Colloids and surfaces. B, Biointerfaces.