Copper oxide nanoparticles impregnated antibacterial surgical gloves for potential application in prevention of nosocomial transmission infections during nursing

This work showed the preparation of Copper oxide nanoparticles (CuO NPs) from leaf extract of Cinnamomum camphora (C.camphora) by a green synthetic method. To prepare CuO NPs, about 10 ml of 0.01 M copper sulphate and 30 ml of C.camphora extract were mixed by heating for 60 min at a temperature of 80 °C. Various techniques such as x-ray diffraction analysis (XRD), Energy-dispersive spectroscopic analysis (EDS), Fourier-transform infrared spectroscopy (FTIR), dynamic light scattering (DLS), UV–vis spectroscopy (UV–vis) and Transmission electron microscopy (TEM) were used for the characterization of biosynthesized CuO NPs. The formation of CuO NPs was indicated by gradual color change of brownish yellow solution into dark brown. Poly-dispersive and spherically shaped NPs were seen from TEM images with an average particle size of about 23 nm. FTIR results confirmed that polyphenols were capped onto the surface of the formed CuO NPs. On the other hand, the Gloves coated with CuO NPs were extremely successful in suppressing contamination of the outside glove surface with nosocomial-resistant microorganisms and hence beneficial of their use in the food sector or clinical context. CuO NPs-coated latex gloves significantly reduced all experimental bacteria within 30 s, including Methicillin-resistant Staphylococcus aureus, Vancomycin resistant enterococci, Escherichia coli, Acinetobacter, and Candida albicans (P <.05). In addition, research must be undertaken to assess the effectiveness of CuO NPs coated gloves in health care setting to know their effectiveness in protection from contaminated fluids that may infiltrate gloves.

[1]  S. H. A. Rizvi,et al.  Synthesis and Characterization of Copper Oxide Nanoparticles by Coprecipitation Method: Electronic and Antimicrobial Properties , 2021 .

[2]  Muhammad Arif Khan,et al.  Surface Study of CuO Nanopetals by Advanced Nanocharacterization Techniques with Enhanced Optical and Catalytic Properties , 2020, Nanomaterials.

[3]  Saranya Sukumar,et al.  Green-Synthesized Rice-Shaped Copper Oxide Nanoparticles Using Caesalpinia bonducella Seed Extract and Their Applications , 2020, ACS omega.

[4]  K. Kathiresan,et al.  Biosynthesis and characterization of copper oxide nanoparticles from indigenous fungi and its effect of photothermolysis on human lung carcinoma. , 2019, Journal of photochemistry and photobiology. B, Biology.

[5]  R. Saravanan,et al.  Towards green synthesis of monodisperse Cu nanoparticles: An efficient and high sensitive electrochemical nitrite sensor , 2018, Sensors and Actuators B: Chemical.

[6]  Silan Chen,et al.  Inhibitory effects of extracts from Cinnamomum camphora fallen leaves on algae. , 2018, Water science and technology : a journal of the International Association on Water Pollution Research.

[7]  B. Mandal,et al.  Biofabrication of size controllable silver nanoparticles - A green approach. , 2017, Journal of photochemistry and photobiology. B, Biology.

[8]  Hany A. Elazab,et al.  Green Synthesis of Copper Oxide Nanoparticles in Aqueous Medium as a Potential Efficient Catalyst for Catalysis Applications , 2017 .

[9]  P. Padma Latha,et al.  Toxicity and Environmental Risks of Nanomaterials : Challenges and Future Needs , 2016 .

[10]  C. Muthukumaran,et al.  Phytofabrication and characterization of monodisperse copper oxide nanoparticles using Albizia lebbeck leaf extract , 2015, Applied Nanoscience.

[11]  Sabu Thomas,et al.  A facile and rapid method for the black pepper leaf mediated green synthesis of silver nanoparticles and the antimicrobial study , 2014, Applied Nanoscience.

[12]  Y. Abboud,et al.  Biosynthesis, characterization and antimicrobial activity of copper oxide nanoparticles (CONPs) produced using brown alga extract (Bifurcaria bifurcata) , 2014, Applied Nanoscience.

[13]  M. Salavati‐Niasari,et al.  Novel solvent-less synthesis of CuO nanoparticles by using sublimated precursors , 2013 .

[14]  Miroslav Černík,et al.  Green synthesis of copper oxide nanoparticles using gum karaya as a biotemplate and their antibacterial application , 2013, International journal of nanomedicine.

[15]  Ritu,et al.  SYNTHESIS OF NANOSIZED CuO PARTICLES : A SIMPLE AND EFFECTIVE METHOD , 2013 .

[16]  L. Rodríguez-Fragoso,et al.  Synthesis, characterization and toxicological evaluation of maltodextrin capped cadmium sulfide nanoparticles in human cell lines and chicken embryos , 2012, Journal of Nanobiotechnology.

[17]  H. Pathan,et al.  Electrochemical growth of copper nanoparticles: Structural and optical properties , 2012 .

[18]  A. Sharma,et al.  Novel route for rapid biosynthesis of copper nanoparticles using aqueous extract of Calotropis procera L. latex and their cytotoxicity on tumor cells. , 2012, Colloids and surfaces. B, Biointerfaces.

[19]  Jongryoul Kim,et al.  Inkjet printing using copper nanoparticles synthesized by electrolysis , 2012 .

[20]  N. Vittayakorn,et al.  Sonochemical Synthesis and Characterization of Copper Oxide Nanoparticles , 2011 .

[21]  K. Haritha.  Extracellular biosynthesis of silver nanoparticles using bacteria. , 2010 .

[22]  N. Perkas,et al.  Pilot scale sonochemical coating of nanoparticles onto textiles to produce biocidal fabrics , 2009 .

[23]  Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food--10 states, 2007. , 2008, MMWR. Morbidity and mortality weekly report.

[24]  Y. Weng,et al.  Intermolecular hydrogen bonds formed between amino acid molecules in aqueous solution investigated by temperature-jump nanosecond time-resolved transient Mid-IR Spectroscopy , 2007 .

[25]  Preliminary FoodNet data on the incidence of infection with pathogens transmitted commonly through food--10 states, 2006. , 2007, MMWR. Morbidity and mortality weekly report.

[26]  James C Mack,et al.  Food worker hand washing practices: an observation study. , 2006, Journal of food protection.

[27]  Hongyuan Chen,et al.  Microwave‐Induced Polyol‐Process Synthesis of Copper and Copper Oxide Nanocrystals with Controllable Morphology , 2004 .

[28]  D. McElwain,et al.  An investigation of contact transmission of methicillin-resistant Staphylococcus aureus. , 2004, The Journal of hospital infection.

[29]  F. Paumgartten,et al.  Comparative study on the susceptibility of freshwater species to copper-based pesticides. , 2004, Chemosphere.

[30]  Curtis J Donskey,et al.  Nosocomial transmission of vancomycin-resistant enterococci from surfaces. , 2002, JAMA.

[31]  A. Kolubaev,et al.  Study of friction reduction by nanocopper additives to motor oil , 2002 .

[32]  D W Schaffner,et al.  Glove barriers to bacterial cross-contamination between hands to food. , 2001, Journal of food protection.

[33]  R A Weinstein,et al.  Effectiveness of gloves in the prevention of hand carriage of vancomycin-resistant enterococcus species by health care workers after patient care. , 2001, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[34]  N. Yamazoe,et al.  Preparation of Stabilized Nanosized Tin Oxide Particles by Hydrothermal Treatment , 2000 .

[35]  S. Hugonnet,et al.  Effectiveness of a hospital-wide programme to improve compliance with hand hygiene , 2000, The Lancet.

[36]  K. Otsuki,et al.  Risk factors in causing outbreaks of food-borne illness originating in schoollunch facilities in Japan. , 2000, The Journal of veterinary medical science.

[37]  Aaron Peled,et al.  Synthesis of nanoparticles in the gas phase for electronic, optical and magnetic applications—a review , 1998 .

[38]  C. Kauffman,et al.  Colonization With Vancomycin-Resistant Enterococcus faecium Comparison of a Long-Term–Care Unit With an Acute-Care Hospital , 1997, Infection Control &#x0026; Hospital Epidemiology.

[39]  J. DeGroot-Kosolcharoen.  Pandemonium over gloves: use and abuse. , 1991, American journal of infection control.