Electrophoresis with electrochemical detection in a polymer microdevice

This work presents the integration of an electrochemical detector in a photoablated polymer microdevice with electroosmotic driven flow. The structure of the device features a separation capillary with two side arms forming the injection capillaries, an electrical decoupler and an electrochemical detector. The decoupler is composed of a microhole or microhole array perpendicular to the separation microchannel. The system allows the electrical high voltage connection between the inlet of the capillary and the decoupler; the remaining end of the separation capillary being almost free of electric field, facilitating an electrochemical detection at the end of the microchannel. Separation parameters have been varied comprising the electrophoretic potential, the time of the injection and the buffer composition. Separation of model phenolic compounds is shown here as a demonstration of the system.

[1]  D. J. Harrison,et al.  Microfabrication of a Planar Absorbance and Fluorescence Cell for Integrated Capillary Electrophoresis Devices , 1996 .

[2]  C. Culbertson,et al.  Microchip Structures for Submillisecond Electrophoresis , 1998 .

[3]  M. I. Montenegro,et al.  Microelectrodes : theory and applications , 1991 .

[4]  J. Fost,et al.  The application of excimer laser micromachining for the fabrication of disc microelectrodes , 1994 .

[5]  Xinjian Huang,et al.  Determination of thiols by capillary electrophoresis with electrochemical detection using a palladium field-decoupler and chemically modified electrodes , 1995 .

[6]  A. Ewing,et al.  Characterization of Electrochemical Array Detection for Continuous Channel Electrophoretic Separations in Micrometer and Submicrometer Channels , 1997 .

[7]  R. Zare,et al.  Use of an on-column frit in capillary zone electrophoresis: sample collection , 1990 .

[8]  Gregor Ocvirk,et al.  Optimization of confocal epifluorescence microscopy for microchip-based miniaturized total analysis systems , 1998 .

[9]  J. Rossier,et al.  Microchannel networks for electrophoretic separations , 1999, Electrophoresis.

[10]  S. Lunte,et al.  A perfluorosulfonated ionomer joint for capillary electrophoresis with on-column electrochemical detection , 1995 .

[11]  D. J. Harrison,et al.  Capillary electrophoresis and sample injection systems integrated on a planar glass chip , 1992 .

[12]  A. Ewing,et al.  Capillary zone electrophoresis with electrochemical detection. , 1987, Analytical chemistry.

[13]  J. Rossier,et al.  UV Laser Machined Polymer Substrates for the Development of Microdiagnostic Systems. , 1997, Analytical chemistry.

[14]  Joël S. Rossier,et al.  Topography, Crystallinity and Wettability of Photoablated PET Surfaces , 1999 .

[15]  R A Mathies,et al.  Capillary electrophoresis chips with integrated electrochemical detection. , 1998, Analytical chemistry.

[16]  C. Lunte,et al.  End-column amperometric detection in capillary electrophoresis: influence of separation-related parameters on the observed half-wave potential for dopamine and catechol. , 1999, Analytical chemistry.

[17]  C. Henry,et al.  Dual-electrode electrochemical detection for poly(dimethylsiloxane)-fabricated capillary electrophoresis microchips. , 2000, Analytical chemistry.

[18]  J Wang,et al.  Integrated electrophoresis chips/amperometric detection with sputtered gold working electrodes. , 1999, Analytical chemistry.

[19]  A. Ewing,et al.  Electrochemical Analysis in Picoliter Microvials , 1997 .

[20]  A. Ewing,et al.  Continuous separations in microfabricated channels for monitoring ultrasmall biological environments , 1997, Nature Medicine.

[21]  F. Matysik.  Improved end-column amperometric detection for capillary electrophoresis , 1996 .

[22]  A. Berg,et al.  Micro Total Analysis Systems , 1995 .

[23]  Jonathan M. Cooper,et al.  Micromachining Sensors for Electrochemical Measurement in Subnanoliter Volumes , 1997 .

[24]  H. Girault,et al.  Amperometric Ion Sensors Based on Laser Patterned Composite Polymer Membranes , 1997 .

[25]  S. F. Li,et al.  Fluorescence detection in short capillary and chip using a variable wavelength epi-fluorescence microscope. , 1998, Talanta.

[26]  J. Rossier,et al.  Electrochemical detection in polymer microchannels. , 1999, Analytical chemistry.

[27]  R A Mathies,et al.  Optimization of high-speed DNA sequencing on microfabricated capillary electrophoresis channels. , 1999, Analytical chemistry.

[28]  Hsuan‐Jung Huang,et al.  An electrochemical cell for end-column amperometric detection in capillary electrophoresis , 1995 .

[29]  C. Whang,et al.  Capillary electrophoresis with amperometric detection using a porous cellulose acetate joint , 1993 .

[30]  J. Michael Ramsey,et al.  Effects of injection schemes and column geometry on the performance of microchip electrophoresis devices , 1994 .

[31]  M. A. Northrup,et al.  Functional integration of PCR amplification and capillary electrophoresis in a microfabricated DNA analysis device. , 1996, Analytical chemistry.

[32]  A. Ewing,et al.  Characterization of Electrochemical Responses in Picoliter Volumes , 1998 .