Title Polymorphic microsatellites in the human bloodfluke , Schistosoma japonicum , identified using a genomic resource Permalink

Re-emergence of schistosomiasis in regions of China where control programs have ceased requires development of molecular-genetic tools to track gene flow and assess genetic diversity of Schistosoma populations. We identified many microsatellite loci in the draft genome of Schistosoma japonicum using defined search criteria and selected a subset for further analysis. From an initial panel of 50 loci, 20 new microsatellites were selected for eventual optimization and application to a panel of worms from endemic areas. All but one of the selected microsatellites contain simple tri-nucleotide repeats. Moderate to high levels of polymorphism were detected. Numbers of alleles ranged from 6 to 14 and observed heterozygosity was always >0.6. The loci reported here will facilitate high resolution population-genetic studies on schistosomes in re-emergent foci.

[1]  Gail M. Williams,et al.  Schistosomiasis in the People's Republic of China: the Era of the Three Gorges Dam , 2010, Clinical Microbiology Reviews.

[2]  X. Zhu,et al.  Sensitive and rapid detection of Schistosoma japonicum DNA by loop-mediated isothermal amplification (LAMP). , 2010, International journal for parasitology.

[3]  Wei Huang,et al.  The Schistosoma japonicum genome reveals features of host–parasite interplay , 2009, Nature.

[4]  Shengyue Wang,et al.  Multiple near-identical genotypes of Schistosoma japonicum can occur in snails and have implications for population-genetic analyses. , 2008, International journal for parasitology.

[5]  J. Remais,et al.  Quantitative Detection of Schistosoma japonicum Cercariae in Water by Real-Time PCR , 2008, PLoS neglected tropical diseases.

[6]  Robert Kofler,et al.  SciRoKo: a new tool for whole genome microsatellite search and investigation , 2007, Bioinform..

[7]  P. Smouse,et al.  genalex 6: genetic analysis in Excel. Population genetic software for teaching and research , 2006 .

[8]  S. Liang,et al.  Re-emerging schistosomiasis in hilly and mountainous areas of Sichuan, China. , 2006, Bulletin of the World Health Organization.

[9]  J. Webster,et al.  An insight into the genetic variation of Schistosoma japonicum in mainland China using DNA microsatellite markers , 2005, Molecular ecology.

[10]  C. Oosterhout,et al.  Micro-Checker: Software for identifying and correcting genotyping errors in microsatellite data , 2004 .

[11]  J. Jakše,et al.  Amplification of fluorescent-labelled microsatellite markers in olives by a novel, economic method , 2004 .

[12]  J. Webster,et al.  Isolation and characterization of polymorphic DNA microsatellite markers from Schistosoma japonicum , 2003 .

[13]  Jerilyn A. Walker,et al.  Preparation of PCR-quality mouse genomic DNA with hot sodium hydroxide and tris (HotSHOT). , 2000, BioTechniques.

[14]  Markus Schuelke,et al.  An economic method for the fluorescent labeling of PCR fragments , 2000, Nature Biotechnology.

[15]  D. Lucena.  Epidemiology of Schistosomiasis due to S. mansoni. , 1950 .