Enhancement of image quality and imaging depth with Airy light-sheet microscopy in cleared and non-cleared neural tissue

We have investigated the effect of Airy illumination on the image quality and depth penetration of digitally scanned light-sheet microscopy in turbid neural tissue. We used Fourier analysis of images acquired using Gaussian and Airy light-sheets to assess their respective image quality versus penetration into the tissue. We observed a three-fold average improvement in image quality at 50 μm depth with the Airy light-sheet. We also used optical clearing to tune the scattering properties of the tissue and found that the improvement when using an Airy light-sheet is greater in the presence of stronger sample-induced aberrations. Finally, we used homogeneous resolution probes in these tissues to quantify absolute depth penetration in cleared samples with each beam type. The Airy light-sheet method extended depth penetration by 30% compared to a Gaussian light-sheet.

[1]  A. Rohrbach,et al.  Light-sheet generation in inhomogeneous media using self-reconstructing beams and the STED-principle. , 2016, Optics express.

[2]  Anna Letizia Allegra Mascaro,et al.  A versatile clearing agent for multi-modal brain imaging , 2015, Scientific Reports.

[3]  David D. Sampson,et al.  Quantifying the influence of Bessel beams on image quality in optical coherence tomography , 2016, Scientific Reports.

[4]  M. Levoy,et al.  Recording and controlling the 4D light field in a microscope using microlens arrays , 2009, Journal of microscopy.

[5]  Jörg Baumgartl,et al.  Optically mediated particle clearing using Airy wavepackets , 2008 .

[6]  J. Eberly,et al.  Experimental investigation of Bessel beam characteristics. , 1992, Applied optics.

[7]  Michael Broxton,et al.  SPED Light Sheet Microscopy: Fast Mapping of Biological System Structure and Function , 2015, Cell.

[8]  K. Deisseroth,et al.  Advanced CLARITY for rapid and high-resolution imaging of intact tissues , 2014, Nature Protocols.

[9]  J. Liu,et al.  Assessing the imaging performance of light sheet microscopies in highly scattering tissues. , 2016, Biomedical optics express.

[10]  F. Del Bene,et al.  Optical Sectioning Deep Inside Live Embryos by Selective Plane Illumination Microscopy , 2004, Science.

[11]  Francesco Saverio Pavone,et al.  Clearing of fixed tissue: a review from a microscopist’s perspective , 2016, Journal of biomedical optics.

[12]  Fengjuan Ye,et al.  Investigating the self-healing property of an optical Airy beam. , 2015, Optics letters.

[13]  Kishan Dholakia,et al.  A compact light-sheet microscope for the study of the mammalian central nervous system , 2016, Scientific Reports.

[14]  Jonathan T. C. Liu,et al.  Characterizing the beam steering and distortion of Gaussian and Bessel beams focused in tissues with microscopic heterogeneities. , 2015, Biomedical optics express.

[15]  Hans-Ulrich Dodt,et al.  Ultramicroscopy: development and outlook , 2015, Neurophotonics.

[16]  Rui-Pin Chen,et al.  Analytical study of the self-healing property of Airy beams , 2012 .

[17]  M. L E V O Y,et al.  Recording and controlling the 4 D light field in a microscope using microlens arrays , 2008 .

[18]  M. Davidson,et al.  Rapid three-dimensional isotropic imaging of living cells using Bessel beam plane illumination , 2011, Nature Methods.

[19]  Peer Wulff,et al.  Production and Titering of Recombinant Adeno-associated Viral Vectors , 2011, Journal of visualized experiments : JoVE.

[20]  A. Rohrbach,et al.  Microscopy with self-reconstructing beams , 2010 .

[21]  K. Dholakia,et al.  Light-sheet microscopy using an Airy beam , 2014, Nature Methods.

[22]  A. Rohrbach,et al.  Propagation stability of self-reconstructing Bessel beams enables contrast-enhanced imaging in thick media , 2012, Nature Communications.

[23]  David Artigas,et al.  Image formation by linear and nonlinear digital scanned light-sheet fluorescence microscopy with Gaussian and Bessel beam profiles , 2012, Biomedical optics express.

[24]  Z. Bouchal,et al.  Self-reconstruction of a distorted nondiffracting beam , 1998 .

[25]  Frank Wyrowski,et al.  Evolution of self-healing characteristic on optical Airy beam , 2014 .

[26]  Philipp J. Keller,et al.  Whole-brain functional imaging at cellular resolution using light-sheet microscopy , 2013, Nature Methods.

[27]  Kishan Dholakia,et al.  A Compact Airy Beam Light Sheet Microscope with a Tilted Cylindrical Lens References and Links , 2022 .

[28]  Kishan Dholakia,et al.  Light beats the spread: “non‐diffracting” beams , 2010 .

[29]  Eric Betzig,et al.  Adaptive optics via pupil segmentation for high-resolution imaging in biological tissues , 2010, Nature Methods.

[30]  D. Christodoulides,et al.  Self-healing properties of optical Airy beams. , 2008, Optics express.