Anthocyanins from cell suspension cultures of Daucus carota.

Six anthocyanins were isolated from cell suspension cultures of an Afghan cultivar of Daucus carota by PC or HPLC. The structures of these compounds were elucidated by spectroscopic methods as cyanidin 3-O-lathyroside, cyanidin 3-O-(2''-O-beta-D-xylopyranosyl-6''-O-beta-D-glucopyranosyl-beta-D- galactopyranoside), and the latter acylated with 4-coumaric, ferulic, 4-hydroxybenzoic or sinapic acid. Unusual 1H NMR chemical shifts and 1H NOE data indicate an intramolecular copigmentation of the aglycone with these aromatic residues.

[1]  Jeffrey B. Harborne,et al.  Methods in plant biochemistry , 1989 .

[2]  A. Bax,et al.  Complete proton and carbon-13 assignments of coenzyme B12 through the use of new two-dimensional NMR experiments , 1986 .

[3]  A. D. Webb,et al.  Structures of Acylated Anthocyan Pigments in Vitis vinifera Variety Tinta Pinheira. Identification of Anthocyanidin, Sugar, and Acid Moieties , 1965 .

[4]  Celestino Santos-Buelga,et al.  High Performance Liquid Chromatography-Diode Array Spectroscopy Identification of Anthocyanins of Vitis vinifera variety Tempranillo , 1988 .

[5]  N. Harada,et al.  Chemistry of Metalloanthocyanins a , 1986 .

[6]  K. Yoshitama,et al.  Selection of a High Anthocyanin-Producing Cell Line of Sweet Potato Cell Cultures and Identification of Pigments , 1987 .

[7]  R. Wrolstad,et al.  Use of HPLC separation:photodiode array detection for characterization of anthocyanins , 1990 .

[8]  K. Norris,et al.  Pelargonidin 3-di (p-hydroxybenzoyl) rutinoside-7-glucoside from flowers of Campanula , 1979 .

[9]  R. Brouillard,et al.  The in vivo expression of anthocyanin colour in plants , 1983 .

[10]  Ø. M. Andersen Chromatographic Separation of Anthocyanins in Cowberry (Lingonberry) Vaccinium vites‐idaea L , 1985 .

[11]  V. Wray,et al.  Structure elucidation of an acylated cyanogenic triglycoside, and further cyanogenic constituents from Xeranthemum cylindraceum , 1990 .

[12]  J. Harborne A Unique pattern of anthocyanins in Daucus carota and other umbelliferae , 1976 .

[13]  Jack D. Henion,et al.  Ion spray interface for combined liquid chromatography/atmospheric pressure ionization mass spectrometry , 1987 .