Bioconversion of de-oiled Jatropha Waste (DJW) to hydrogen and methane gas by anaerobic fermentation: Influence of substrate concentration, temperature and pH

Cellulosic materials-based de-oiled Jatropha Waste (DJW) was fermented to H-2 and CH4 using sewage sludge inoculum. Batch assays were performed at various substrate concentrations (40-240 g/L), temperatures (25-65 degrees C) and pHs (5.5-7.5). The peak hydrogen production rate (HPR) and hydrogen yield (HY) of 744.0 +/- 11.3 mL H-2/L-d and 10.6 +/- 0.2 mL H-2/g VS obtained when the optimal substrate concentration, pH, temperature were 200 g/L, 6.5, 55 degrees C, respectively. The peak methane production rate (MPR) of 178.4 +/- 5.6 mL CH4/L-d obtained while DJW concentration, pH, temperature were 200 g/L, 7.0, 45 degrees C, however, peak methane yield (MY) of 23.3 +/- 0.1 mL CH4/g VS obtained at 40 g/L, 7.0 and 55 degrees C, respectively. Effect of substrate concentration on HPR and MPR was elucidated using Monod model. Butyrate and acetate were the main soluble metabolic products. Maximal carbohydrate removal and COD reduction were achieved as 51.7 +/- 0.7% and 68.3 +/- 1.6%, respectively. Copyright (c) 2012, Hydrogen Energy Publications, LLC. Published by Elsevier Ltd. All rights reserved.

[1]  P. Mccarty,et al.  Bioassay for monitoring biochemical methane potential and anaerobic toxicity , 1979 .

[2]  Sang Jun Sim,et al.  High-yield biohydrogen production from biodiesel manufacturing waste by Thermotoga neapolitana , 2011 .

[3]  John A. Turner,et al.  Sustainable Hydrogen Production , 2004, Science.

[4]  Samir Kumar Khanal,et al.  Biological hydrogen production: effects of pH and intermediate products , 2003 .

[5]  S. Venkata Mohan,et al.  Biohydrogen production from chemical wastewater as substrate by selectively enriched anaerobic mixed consortia: Influence of fermentation pH and substrate composition , 2007 .

[6]  C. Chu,et al.  Seed inocula for biohydrogen production from biodiesel solid residues , 2012 .

[7]  A. Ogino,et al.  Community analysis of hydrogen-producing extreme thermophilic anaerobic microflora enriched from cow manure with five substrates , 2007, Applied Microbiology and Biotechnology.

[8]  Jaakko A Puhakka,et al.  The relationship between instability of H2 production and compositions of bacterial communities within a dark fermentation fluidised‐bed bioreactor , 2007, Biotechnology and bioengineering.

[9]  Yutaka Nakashimada,et al.  Hydrogen and ethanol production from glycerol-containing wastes discharged after biodiesel manufacturing process. , 2005, Journal of bioscience and bioengineering.

[10]  Eun Yeol Lee,et al.  Fermentative hydrogen production by a new chemoheterotrophic bacterium Rhodopseudomonas palustris P4 , 2002 .

[11]  Liling Wei,et al.  Biohydrogen production from poplar leaves pretreated by different methods using anaerobic mixed bacteria , 2010 .

[12]  Tatsuya Noike,et al.  CHARACTERISTICS OF CELLULOSE AND GLUCOSE DECOMPOSITION IN ACIDOGENIC PHASE OF ANAEROBIC DIGESTION , 1982 .

[13]  T. Sreekrishnan,et al.  Anaerobic treatment of agricultural residue based pulp and paper mill effluents for AOX and COD reduction , 2000 .

[14]  Jianlong Wang,et al.  FACTORS INFLUENCING FERMENTATIVE HYDROGEN PRODUCTION: A REVIEW , 2009 .

[15]  J. Jones,et al.  Production of acetic acid by Clostridium thermoaceticum in batch and continuous fermentations , 1986, Biotechnology and bioengineering.

[16]  Chin-Chao Chen,et al.  Biohydrogen and biomethane from water hyacinth ( Eichhornia crassipes) fermentation: Effects of subs , 2011 .

[17]  Godfrey Kyazze,et al.  Continuous dark fermentative hydrogen production by mesophilic microflora: principles and progress , 2007 .

[18]  V. Vavilin,et al.  Modelling hydrogen partial pressure change as a result of competition between the butyric and propionic groups of acidogenic bacteria , 1995 .

[19]  Irini Angelidaki,et al.  Hydrogen and methane production from household solid waste in the two-stage fermentation process. , 2006, Water research.

[20]  H. Argun,et al.  Batch dark fermentation of powdered wheat starch to hydrogen gas : Effects of the initial substrate and biomass concentrations , 2008 .

[21]  L. Daniels,et al.  Effect of pH on Anaerobic Mild Steel Corrosion by Methanogenic Bacteria , 1991, Applied and environmental microbiology.

[22]  Jan A. Oleszkiewicz,et al.  Anaerobic co‐composting of municipal solid waste and waste sludge at hlgh total solids levels , 1992 .

[23]  Chiu-Yue Lin,et al.  Effects of initial cultivation pH on fermentative hydrogen production from xylose using natural mixed cultures , 2006 .

[24]  Debabrata Das,et al.  Hydrogen production by biological processes: a survey of literature , 2001 .

[25]  Debabrata Das,et al.  Improvement of fermentative hydrogen production: various approaches , 2004, Applied Microbiology and Biotechnology.

[26]  Xiaohui Xu,et al.  Hydrogen and methane production from lipid-extracted microalgal biomass residues , 2011 .

[27]  E. Ferreira,et al.  Inoculum type response to different pHs on biohydrogen production from L-arabinose, a component of hemicellulosic biopolymers , 2009 .

[28]  P. Rogers.  Genetics and Biochemistry of Clostridium Relevant to Development of Fermentation Processes , 1986 .

[29]  Hubert Bahl,et al.  Parameters Affecting Solvent Production by Clostridium pasteurianum , 1992, Applied and environmental microbiology.

[30]  S. Jayaraj,et al.  Characterization and Gasification using- Jatropha Curcas Seed Cake , 2010 .

[31]  Chun-Hsiung Hung,et al.  Temperature effects on fermentative hydrogen production from xylose using mixed anaerobic cultures , 2008 .

[32]  L. H. Koehler.  Differentiation of carbohydrates by anthrone reaction rate and color intensity. , 1952 .

[33]  Sangeun Oh,et al.  The relative effectiveness of pH control and heat treatment for enhancing biohydrogen gas production. , 2003, Environmental science & technology.

[34]  E. Crabbe,et al.  Influence of initial pH on hydrogen production from cheese whey. , 2005, Journal of biotechnology.

[35]  D. Boone,et al.  Methanosarcina mazei LYC, a New Methanogenic Isolate Which Produces a Disaggregating Enzyme , 1985, Applied and environmental microbiology.

[36]  Debabrata Das,et al.  Improvement of biohydrogen production by Enterobacter cloacae IIT-BT 08 under regulated pH. , 2011, Journal of biotechnology.

[37]  Bruno Fabiano,et al.  Thermodynamic study and optimization of hydrogen production by Enterobacter aerogenes , 2002 .

[38]  Duangduen Atong,et al.  Thermal decomposition study on Jatropha curcas L. waste using TGA and fixed bed reactor , 2009 .

[39]  Yu-You Li,et al.  A pH- and temperature-phased two-stage process for hydrogen and methane production from food waste , 2008 .

[40]  Jianlong Wang,et al.  Kinetic models for fermentative hydrogen production: A review , 2009 .

[41]  Tong Zhang,et al.  Thermophilic H2 production from a cellulose-containing wastewater , 2003, Biotechnology Letters.