Short-term mineralization of dentin and enamel in the mouse embryonic molars cultured in serum-free, chemically-defined medium.

The present study was designed to demonstrate the short term mineralization of dentin and enamel, and to investigate the effects of sodium beta-glycerophosphate (Na-beta-GPO4) on calcification in a serum-free, chemically-defined medium. The first mandibular molars at the bell stage dissected from 18-day-old mouse embryos were used as explants, which were cultured by an improved flotation method. Calcification of enamel in the 18-day-old embryonic molars occurred within the 6th day of culture. In another experiment, the molar germs were cultured in a serum-free, chemically-defined medium supplemented with 1, 5 and 10 mM Na-beta-GPO4. Promotion of tooth mineralization was recognizable at very low concentrations, such as 1 mM Na-beta-GPO4, in 18-day-old embryonic tooth germs. The culturing system reported here shortens the time required for dentin and enamel calcification to one half or one third of that reported previously and therefore should prove useful for examining regulations for cytodifferentiation and morphogenesis in tooth germs and the mineralization of dentin and enamel.

[1]  H. Slavkin.  Gene Regulation in the Development of Oral Tissues , 1988, Journal of dental research.

[2]  A. Bronckers,et al.  Effects of Calcium and Phosphate On Secretion of Enamel Matrix and Its Subsequent Mineralization in Vitro , 1987, Advances in dental research.

[3]  H. Slavkin,et al.  Ultrastructural analysis of enamel formation during in vitro development using chemically-defined medium. , 1987, Scanning microscopy.

[4]  J B Lian,et al.  Expression of differentiated function by mineralizing cultures of chicken osteoblasts. , 1987, Developmental biology.

[5]  D. Coomans,et al.  Characteristics of mineralization of rat molar tooth germs in organ culture. , 1986, Journal de biologie buccale.

[6]  I. Thesleff,et al.  Development of mouse embryonic molars in vitro: an attempt to design defined culture conditions allowing mineralization. , 1986, Journal de biologie buccale.

[7]  Bronckers Al.  Effect of oxygen tension on matrix formation and mineralization in hamster molars during development in vitro. , 1983 .

[8]  Thomas M. Graber,et al.  Factors and mechanisms influencing bone growth , 1983 .

[9]  L. Honig,et al.  Antibodies to murine amelogenins: localization of enamel proteins during tooth organ development in vitro. , 1982, Differentiation; research in biological diversity.

[10]  A. Bronckers,et al.  Amelogenesis and mineralization in vitro , 1981 .

[11]  Howard C. Tenenbaum.  Role of organic phosphate in mineralization of bone in vitro. , 1981, Journal of dental research.

[12]  H. Slavkin,et al.  Chemically-defined organ culture of embryonic mouse tooth organs: morphogenesis, dentinogenesis and amelogenesis. , 1980, Journal de biologie buccale.

[13]  H. Slavkin.  Amelogenesis In Vitro , 1979, Journal of dental research.

[14]  I. Thesleff.  Differentiation of odontogenic tissues in organ culture. , 1976, Scandinavian journal of dental research.

[15]  G. Levenson.  Effect of ascorbic acid deficiency on mouse second molar tooth germs cultivated in vitro. , 1976, Journal of embryology and experimental morphology.

[16]  M. Hay.  The development in vivo and in vitro of the lower incisor and molars of the mouse. , 1961, Archives of oral biology.

[17]  H. Slavkin,et al.  Cartilage, bone and tooth induction during early embryonic mouse mandibular morphogenesis using serumless, chemically-defined medium. , 1990, Connective tissue research.

[18]  H. Slavkin,et al.  Hertwig's epithelial root sheath differentiation and initial cementum and bone formation during long-term organ culture of mouse mandibular first molars using serumless, chemically-defined medium. , 1989, Journal of periodontal research.

[19]  H. Slavkin,et al.  Early embryonic mouse mandibular morphogenesis and cytodifferentiation in serumless, chemically defined medium: a model for studies of autocrine and/or paracrine regulatory factors. , 1989, Journal of craniofacial genetics and developmental biology.

[20]  A. Bronckers,et al.  Effect of developmental stage of explants on further in-vitro development of hamster molars. , 1983, Archives of oral biology.

[21]  A. Bronckers.  A histological and biochemical study of the effect of vitamin C-deficiency on induction of amelogenesis in hamster molars in vitro. , 1983, Archives of oral biology.

[22]  L. Honig,et al.  Experimental dissection of avian and murine tissue interactions using organ culture in a serumless medium free from exogenous (nondefined) factors. , 1982, Progress in clinical and biological research.

[23]  D. Wigglesworth.  Formation and mineralisation of enamel and dentine by rat tooth germs in vitro. , 1968, Experimental cell research.