Feeding Calcium-Ammonium Nitrate to Lactating Dairy Goats: Milk Quality and Ruminal Fermentation Responses

Simple Summary Calcium-ammonium nitrate (CAN) has been extensively used as a potential methane inhibitor for ruminants; however, there is still a need for studies focused on investigating its effects on the fatty acid profile and antioxidant capacity of milk, especially from dairy goats. Therefore, we evaluated the effects of CAN on nutrient digestibility, ruminal fermentation, and milk quality of lactating Saanen goats. Treatments consisted of a control diet (without CAN), 10 g of CAN per kg of dry matter, and 20 g of CAN per kg of dry matter. Supplemental CAN did not affect feed intake, digestibility of nutrients, and most ruminal fermentation parameters. Yields and composition of milk were not affected, and minor treatment effects were observed on the milk fatty acid profile. Milk antioxidant capacity was altered by increased conjugated dienes and reduced thiobarbituric acid reactive substances, along with greater concentrations of nitrate and nitrite residues in milk. Calcium-ammonium nitrate can be fed to lactating dairy goats up to 20 g per kg of dry matter without negative effects on nutrient digestibility and milk composition; however, it increased the concentration of conjugated dienes in milk, which may induce its faster lipid oxidation. Abstract We aimed to investigate the effects of calcium-ammonium nitrate (CAN) fed to lactating dairy goats on dry matter (DM) intake, digestibility of nutrients, milk properties (composition, antioxidant capacity, fatty acid profile, and nitrate residues), and ruminal fermentation parameters. Twelve lactating Saanen goats averaging 98.5 ± 13.1 days in milk, 53.5 ± 3.3 kg of body weight, and 2.53 ± 0.34 kg of milk/day were randomly assigned in four 3 × 3 Latin squares to receive the following diets: a control group (without CAN) with 7.3 g/kg DM of urea (URE), 10 g/kg DM of CAN (CAN10), and 20 g/kg DM of CAN (CAN20). Each period lasted 21 days, with 14 days for diet adaptation and seven days for data and sample collection. The DM intake, digestibility of nutrients, yields of milk, 3.5% fat-corrected milk, and energy-corrected milk were not affected by treatments. Similarly, there were no treatment effects on the yields and concentrations of milk fat, true protein, and lactose, along with minor effects on milk fatty acid profile. Total antioxidant capacity in milk was unaffected by treatments; however, concentration of conjugated dienes increased, while thiobarbituric acid reactive substances in milk decreased linearly. Nitrate and nitrite residues in milk were elevated by treatments, while the total of volatile fatty acids and ammonia-N concentration in the rumen were unaffected. Collectively, feeding CAN (up to 20 g/kg of DM) to lactating dairy goats did not affect feed intake, nutrient digestibility, and milk composition; however, it may increase milk lipid oxidation, as evidenced by increased conjugated diene concentration.

[1]  G. T. Santos,et al.  Effects of calcium ammonium nitrate fed to dairy cows on nutrient intake and digestibility, milk quality, microbial protein synthesis, and ruminal fermentation parameters. , 2022, Journal of dairy science.

[2]  J. Daniel,et al.  Annatto seeds as Antioxidants Source with Linseed Oil for Dairy Cows , 2021, Animals : an open access journal from MDPI.

[3]  D. Erler,et al.  Nitrate and nitrite absorption, recycling and retention in tissues of sheep , 2021, Small Ruminant Research.

[4]  J. France,et al.  Antimethanogenic effects of nitrate supplementation in cattle: A meta-analysis. , 2020, Journal of dairy science.

[5]  K. Beauchemin,et al.  Review: Fifty years of research on rumen methanogenesis: lessons learned and future challenges for mitigation. , 2020, Animal : an international journal of animal bioscience.

[6]  D. Sauvant,et al.  Rumen function in goats, an example of adaptive capacity , 2020, Journal of Dairy Research.

[7]  A. Gehman,et al.  Potential roles of nitrate and live yeast culture in suppressing methane emission and influencing ruminal fermentation, digestibility, and milk production in lactating Jersey cows. , 2019, Journal of dairy science.

[8]  R. Wallace,et al.  Consequences of inhibiting methanogenesis on the biohydrogenation of fatty acids in bovine ruminal digesta , 2019, Animal Feed Science and Technology.

[9]  M. Fernandes,et al.  Energy partition and nitrogen utilization by male goats fed encapsulated calcium nitrate as a replacement for soybean meal , 2019, Animal Feed Science and Technology.

[10]  D. Morgavi,et al.  Changes in the Rumen Microbiota of Cows in Response to Dietary Supplementation with Nitrate, Linseed, and Saponin Alone or in Combination , 2018, Applied and Environmental Microbiology.

[11]  Min Wang,et al.  Nitrate improves ammonia incorporation into rumen microbial protein in lactating dairy cows fed a low-protein diet. , 2018, Journal of dairy science.

[12]  S. Clark,et al.  A 100-Year Review: Advances in goat milk research. , 2017, Journal of dairy science.

[13]  J. Dijkstra,et al.  Effect of dietary nitrate level on enteric methane production, hydrogen emission, rumen fermentation, and nutrient digestibility in dairy cows. , 2016, Journal of dairy science.

[14]  W. E. Pinchak,et al.  Insights on Alterations to the Rumen Ecosystem by Nitrate and Nitrocompounds , 2016, Front. Microbiol..

[15]  J. Dijkstra,et al.  Feeding nitrate and docosahexaenoic acid affects enteric methane production and milk fatty acid composition in lactating dairy cows. , 2016, Journal of dairy science.

[16]  C. Martin,et al.  Long-term effect of linseed plus nitrate fed to dairy cows on enteric methane emission and nitrate and nitrite residuals in milk. , 2016, Animal : an international journal of animal bioscience.

[17]  N. Asanuma,et al.  Effects of nitrate addition to a diet on fermentation and microbial populations in the rumen of goats, with special reference to Selenomonas ruminantium having the ability to reduce nitrate and nitrite. , 2015, Animal science journal = Nihon chikusan Gakkaiho.

[18]  K. Beauchemin,et al.  A review of feeding supplementary nitrate to ruminant animals: nitrate toxicity, methane emissions, and production performance , 2014 .

[19]  M. Barnett,et al.  Use of nitrate and Propionibacterium acidipropionici to reduce methane emissions and increase wool growth of Merino sheep , 2014 .

[20]  G. Leitner,et al.  Subclinical mastitis in goats is associated with upregulation of nitric oxide-derived oxidative stress that causes reduction of milk antioxidative properties and impairment of its quality. , 2014, Journal of dairy science.

[21]  Antonio Ayala,et al.  Lipid Peroxidation: Production, Metabolism, and Signaling Mechanisms of Malondialdehyde and 4-Hydroxy-2-Nonenal , 2014, Oxidative medicine and cellular longevity.

[22]  J. Dorne,et al.  Nitrite in feed: from animal health to human health. , 2013, Toxicology and applied pharmacology.

[23]  G. Leitner,et al.  Lipopolysaccharide challenge of the mammary gland in cows induces nitrosative stress that impairs milk oxidative stability. , 2012, Animal : an international journal of animal bioscience.

[24]  V. Fievez,et al.  Milk odd- and branched-chain fatty acids as biomarkers of rumen function—An update , 2012 .

[25]  N. Bryan,et al.  Nitrate and nitrite content of human, formula, bovine, and soy milks: implications for dietary nitrite and nitrate recommendations. , 2011, Breastfeeding medicine : the official journal of the Academy of Breastfeeding Medicine.

[26]  J. Dijkstra,et al.  Persistency of methane mitigation by dietary nitrate supplementation in dairy cows. , 2011, Journal of dairy science.

[27]  J. Dijkstra,et al.  Nitrate and sulfate: Effective alternative hydrogen sinks for mitigation of ruminal methane production in sheep. , 2010, Journal of dairy science.

[28]  G. Leitner,et al.  Hydrogen peroxide-dependent conversion of nitrite to nitrate as a crucial feature of bovine milk catalase. , 2009, Journal of agricultural and food chemistry.

[29]  H. L. Månsson,et al.  Fatty acids in bovine milk fat , 2008, Food & nutrition research.

[30]  C.F.A.M. Penna,et al.  Características microbiológicas e físico-químicas do leite de cabra submetido à pasteurização lenta pós-envase e ao congelamento , 2008 .

[31]  V. Oreopoulou,et al.  Effect of Compositional Factors against the Thermal Oxidative Deterioration of Novel Food Emulsions , 2006 .

[32]  Hong-yu Zhang,et al.  Estimation of scavenging activity of phenolic compounds using the ABTS(*+) assay. , 2004, Journal of agricultural and food chemistry.

[33]  J. Brito,et al.  Emprego do Somacount 300, calibrado com leite de vaca, na contagem de células somáticas no leite de cabra , 2004 .

[34]  M. Guillén,et al.  Fourier transform infrared spectra data versus peroxide and anisidine values to determine oxidative stability of edible oils , 2002 .

[35]  S. J. Jadhav,et al.  Lipid Oxidation in Biological and Food Systems , 1995 .

[36]  D. Sklan,et al.  Fatty acids, calcium soaps of fatty acids, and cottonseeds fed to high yielding cows. , 1992, Journal of dairy science.

[37]  J. Pedersen,et al.  A Nordic proposal for an energy corrected milk (ECM) formula , 1991 .

[38]  N. Cortas,et al.  Determination of inorganic nitrate in serum and urine by a kinetic cadmium-reduction method. , 1990, Clinical chemistry.

[39]  J. D. Wallace,et al.  Predicting Digestibility of Different Diets with Internal Markers: Evaluation of Four Potential Markers , 1986 .

[40]  P. Bickel,et al.  An Analysis of Transformations Revisited , 1981 .

[41]  R. Hegarty,et al.  The effect of dietary nitrate and canola oil alone or in combination on fermentation, digesta kinetics and methane emissions from cattle , 2020, Animal Feed Science and Technology.

[42]  P. Polidori,et al.  Rapid Assay to Evaluate the Total Antioxidant Capacity in Donkey Milk and in more Common Animal Milk for Human Consumption , 2016 .

[43]  Q. Meng,et al.  Effects of nitrate on methane production, fermentation, and microbial populations in in vitro ruminal cultures. , 2012, Bioresource technology.

[44]  AO Raleng THE POTENTIAL OF FEEDING NITRATE TO REDUCE ENTERIC METHANE PRODUCTION IN RUMINANTS , 2008 .

[45]  A. Pedersen,et al.  How to Obtain Those Nasty Standard Errors from Transformed Data | and Why They Should Not Be Used , 2007 .

[46]  P. Parodi Milk fat in human nutrition , 2004 .

[47]  J. Murphya,et al.  Effects on milk fat composition and cow performance of feeding concentrates containing full fat rapeseed and maize distillers grains on grass-silage based diets , 2003 .

[48]  G. Broderick,et al.  Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media. , 1980, Journal of dairy science.

[49]  W. Vyncke Direct Determination of the Thiobarbituric Acid Value in Trichloracetic Acid Extracts of Fish as a Measure of Oxidative Rancidity , 1970 .