138 2′-Deoxyguanine analogs which enhance hybridization oligonucleotides with complementary DNA

et al., 2014). Here we present the results of the SER titration via an excess of competing oligonucleotide, according to (Reynaldo, Vologodskij, Neri, & Lyamichev, 2000). The sensitivity of the HG122 promoted SER to limited heterology between the reaction substrates was also estimated. As seen from the Figure, both HG122 and Rec A protein promote SER. In both cases, SER exhibits decreased sensibility to homology violation unlike SER promoted by other agents, such as linker histone H1, or mammalian meiotic system protein Hop2. The latter exhibit the same sensitivity to heterology as that of spontaneous SER at increased temperature (Bocharova, Smirnova, & Volodin, 2012; Pezza et al., 2014). Thus, 1,3-diazaadamantane derivative HG122 acts similar to recombinase Rec A family. This ligand facilitates SER, destabilizes the DNA duplex and appreciably reduces SER sensitivity to heterology between SER substrates. Therefore, compounds of this class are worthy models for further study of different aspects of SER mechanisms.