Reverse Engineering Human Pathophysiology with Organs-on-Chips

While studies of cultured cells have led to new insights into biological control, greater understanding of human pathophysiology requires the development of experimental systems that permit analysis of intercellular communications and tissue-tissue interactions in a more relevant organ context. Human organs-on-chips offer a potentially powerful new approach to confront this long-standing problem.

[1]  Mandy B. Esch,et al.  Body-on-a-chip simulation with gastrointestinal tract and liver tissues suggests that ingested nanoparticles have the potential to cause liver injury. , 2014, Lab on a chip.

[2]  L. Christophorou Science , 2018, Emerging Dynamics: Science, Energy, Society and Values.

[3]  Donald E. Ingber,et al.  Distinct Contributions of Astrocytes and Pericytes to Neuroinflammation Identified in a 3D Human Blood-Brain Barrier on a Chip , 2016, PloS one.

[4]  Thomas C. Ferrante,et al.  Small airway-on-a-chip enables analysis of human lung inflammation and drug responses in vitro , 2015, Nature Methods.

[5]  D. Ingber,et al.  Microfluidic organs-on-chips , 2014, Nature Biotechnology.

[6]  D. Ingber,et al.  Reconstituting Organ-Level Lung Functions on a Chip , 2010, Science.

[7]  J. Cuevas,et al.  A modular approach to create a neurovascular unit-on-a-chip. , 2013, Lab on a chip.

[8]  David J Beebe,et al.  Microfluidic model of ductal carcinoma in situ with 3D, organotypic structure , 2015, BMC Cancer.

[9]  R. Rosenfeld Nature , 2009, Otolaryngology--head and neck surgery : official journal of American Academy of Otolaryngology-Head and Neck Surgery.

[10]  Leila Mohammadi,et al.  BMC Cancer , 2001 .

[11]  T. Hartung,et al.  Inflammatory findings on species extrapolations: humans are definitely no 70-kg mice , 2013, Archives of Toxicology.

[12]  F. Sonntag,et al.  A four-organ-chip for interconnected long-term co-culture of human intestine, liver, skin and kidney equivalents. , 2015, Lab on a chip.

[13]  J. Collins,et al.  Contributions of microbiome and mechanical deformation to intestinal bacterial overgrowth and inflammation in a human gut-on-a-chip , 2015, Proceedings of the National Academy of Sciences.

[14]  Madeline A. Lancaster,et al.  Cerebral organoids model human brain development and microcephaly , 2013, Nature.

[15]  Shuichi Takayama,et al.  Fabrication of two-layered channel system with embedded electrodes to measure resistance across epithelial and endothelial barriers. , 2010, Analytical chemistry.

[16]  Daniel C Leslie,et al.  A Human Disease Model of Drug Toxicity–Induced Pulmonary Edema in a Lung-on-a-Chip Microdevice , 2012, Science Translational Medicine.

[17]  D. Ingber,et al.  Human gut-on-a-chip inhabited by microbial flora that experiences intestinal peristalsis-like motions and flow. , 2012, Lab on a chip.

[18]  H. Clevers,et al.  Growing Self-Organizing Mini-Guts from a Single Intestinal Stem Cell: Mechanism and Applications , 2013, Science.