Diffusion-tensor neuronal fiber tractography and manganese-enhanced MR imaging of primate visual pathway in the common marmoset: preliminary results.

PURPOSE To investigate whether diffusion-tensor tractography (DTT) of neuronal fibers is useful for delineating the configuration of the neuronal fiber trajectories in the primate visual pathway, including the well-developed optic chiasm, in comparison with tract tracing at manganese-enhanced magnetic resonance (MR) imaging. MATERIALS AND METHODS The handling methods used for all the animals in this study were approved by the institutional committee for animal experiments. Diffusion-tensor MR imaging was performed in four healthy common marmosets, and in two of these animals, manganese-enhanced MR imaging tract tracing was performed by using a 7.0-T MR imaging unit. The visual pathways were quantitatively investigated in terms of the manganese distribution observed on the manganese-enhanced MR images. The images obtained with DTT and manganese-enhanced MR imaging tract tracing were qualitatively compared, and the features of the visual pathway were verified through fusion of the reconstructed images obtained by using these two modalities. RESULTS DTT provided information regarding the neuroanatomic features of the marmoset visual pathway and revealed the bilateral branching patterns of the typical primate retinogeniculate pathways, although several incorrectly tracked fibers were noted. The distribution of manganese on the manganese-enhanced MR images revealed bilateral innervation of the retinal projections and depicted the layered internal structure of the lateral geniculate nuclei bilaterally, depending on the ocularity of each layer. These morphologic findings were consistent with those of previous histopathologic studies. CONCLUSION The findings of this preliminary study raise the possibility that DTT is useful for visualizing the neuronal fiber trajectories in primate visual pathways. SUPPLEMENTAL MATERIAL http://radiology.rsnajnls.org/cgi/content/full/249/3/855/DC1.

[1]  D. Le Bihan,et al.  From the diffusion coefficient to the diffusion tensor , 2002, NMR in biomedicine.

[2]  S. Mori,et al.  Principles of Diffusion Tensor Imaging and Its Applications to Basic Neuroscience Research , 2006, Neuron.

[3]  C. Holt,et al.  Control of retinal growth and axon divergence at the chiasm: lessons from Xenopus , 2001, BioEssays : news and reviews in molecular, cellular and developmental biology.

[4]  M. Horsfield,et al.  Optimal strategies for measuring diffusion in anisotropic systems by magnetic resonance imaging , 1999, Magnetic resonance in medicine.

[5]  J. Tsuruda,et al.  Diffusion-weighted MR imaging of anisotropic water diffusion in cat central nervous system. , 1990, Radiology.

[6]  C Kupfer,et al.  Quantitative histology of optic nerve, optic tract and lateral geniculate nucleus of man. , 1967, Journal of anatomy.

[7]  D. Parker,et al.  Analysis of partial volume effects in diffusion‐tensor MRI , 2001, Magnetic resonance in medicine.

[8]  Ching-Po Lin,et al.  Validation of Diffusion Tensor Magnetic Resonance Axonal Fiber Imaging with Registered Manganese-Enhanced Optic Tracts , 2001, NeuroImage.

[9]  J. Naito.  Retinogeniculate projection fibers in the monkey optic nerve: A demonstration of the fiber pathways by retrograde axonal transport of WGA‐HRP , 1989, The Journal of comparative neurology.

[10]  R. Colman,et al.  Aspects of common marmoset basic biology and life history important for biomedical research. , 2003, Comparative medicine.

[11]  W. Hoyt,et al.  The primate chiasm. Details of visual fiber organization studied by silver impregnation techniques. , 1963, Archives of ophthalmology.

[12]  W. B. Spatz.  The retino-geniculo-cortical pathway in callithrix I. Intraspecific variations in the lamination pattern of the lateral geniculate nucleus , 1978, Experimental Brain Research.

[13]  O. Abe,et al.  MR diffusion tensor imaging: recent advance and new techniques for diffusion tensor visualization. , 2003, European journal of radiology.

[14]  V. Wedeen,et al.  Diffusion MRI of Complex Neural Architecture , 2003, Neuron.

[15]  N. Makris,et al.  High angular resolution diffusion imaging reveals intravoxel white matter fiber heterogeneity , 2002, Magnetic resonance in medicine.

[16]  Sinisa Pajevic,et al.  Color schemes to represent the orientation of anisotropic tissues from diffusion tensor data: Application to white matter fiber tract mapping in the human brain , 1999, Magnetic resonance in medicine.

[17]  Yusuke Murayama,et al.  Tracing neural circuits in vivo with Mn-enhanced MRI. , 2006, Magnetic resonance imaging.

[18]  Peter Boesiger,et al.  Reconstruction of the human visual system based on DTI fiber tracking , 2007, Journal of magnetic resonance imaging : JMRI.

[19]  W. Hoyt,et al.  Visual fiber anatomy in the infrageniculate pathway of the primate. , 1962, Archives of ophthalmology.

[20]  Neil R Miller.  Diffusion tensor imaging of the visual sensory pathway: are we there yet? , 2005, American journal of ophthalmology.

[21]  Afonso C. Silva,et al.  In vivo neuronal tract tracing using manganese‐enhanced magnetic resonance imaging , 1998, Magnetic resonance in medicine.

[22]  S. Skare,et al.  Noise considerations in the determination of diffusion tensor anisotropy. , 2000, Magnetic resonance imaging.

[23]  M. Magnin,et al.  Retinohypothalamic pathway: A breach in the law of Newton-Müller-Gudden? , 1989, Brain Research.

[24]  Derek K. Jones,et al.  Diffusion‐tensor MRI: theory, experimental design and data analysis – a technical review , 2002 .

[25]  Susumu Mori,et al.  Fiber tracking: principles and strategies – a technical review , 2002, NMR in biomedicine.

[26]  Axel Sandvig,et al.  Manganese‐enhanced MRI of the optic visual pathway and optic nerve injury in adult rats , 2005, Journal of magnetic resonance imaging : JMRI.

[27]  Ichio Aoki,et al.  Manganese‐enhanced magnetic resonance imaging (MEMRI): methodological and practical considerations , 2004, NMR in biomedicine.

[28]  E. Stejskal.  Use of Spin Echoes in a Pulsed Magnetic‐Field Gradient to Study Anisotropic, Restricted Diffusion and Flow , 1965 .

[29]  Mehmet Bilgen.  Imaging corticospinal tract connectivity in injured rat spinal cord using manganese-enhanced MRI , 2006, BMC Medical Imaging.

[30]  Afonso C. Silva,et al.  Manganese‐enhanced magnetic resonance imaging (MEMRI) , 2004, NMR in biomedicine.

[31]  Denis Le Bihan,et al.  From the diffusion coefficient to the diffusion tensor. , 2002 .

[32]  D. Tuch.  Q‐ball imaging , 2004, Magnetic resonance in medicine.

[33]  J. Frahm,et al.  Mapping of retinal projections in the living rat using high‐resolution 3D gradient‐echo MRI with Mn2+‐induced contrast , 2001, Magnetic resonance in medicine.

[34]  P. Grenier,et al.  MR imaging of intravoxel incoherent motions: application to diffusion and perfusion in neurologic disorders. , 1986, Radiology.

[35]  S Skare,et al.  Condition number as a measure of noise performance of diffusion tensor data acquisition schemes with MRI. , 2000, Journal of magnetic resonance.

[36]  V. Wedeen,et al.  Fiber crossing in human brain depicted with diffusion tensor MR imaging. , 2000, Radiology.

[37]  J. E. Tanner,et al.  Spin diffusion measurements : spin echoes in the presence of a time-dependent field gradient , 1965 .

[38]  J Naito,et al.  Retinogeniculate projection fibers in the monkey optic chiasm: A demonstration of the fiber arrangement by means of wheat germ agglutinin conjugated to horseradish peroxidase , 1994, The Journal of comparative neurology.