The utility of naphthyl-keratin adducts as biomarkers for jet-fuel exposure

We investigated the association between biomarkers of dermal exposure, naphthyl-keratin adducts (NKA), and urine naphthalene biomarker levels in 105 workers routinely exposed to jet-fuel. A moderate correlation was observed between NKA and urine naphthalene levels (p = 0.061). The NKA, post-exposure breath naphthalene, and male gender were associated with an increase, while CYP2E1*6 DD and GSTT1-plus (++/+−) genotypes were associated with a decrease in urine naphthalene level (p < 0.0001). The NKA show great promise as biomarkers for dermal exposure to naphthalene. Further studies are warranted to characterize the relationship between NKA, other exposure biomarkers, and/or biomarkers of biological effects due to naphthalene and/or PAH exposure.

[1]  R. Shore,et al.  Albumin adducts of benzene oxide and 1,4-benzoquinone as measures of human benzene metabolism. , 2002, Cancer research.

[2]  Roger Gibson,et al.  Dose-dependent production of urinary naphthols among workers exposed to jet fuel (JP-8). , 2004, American journal of industrial medicine.

[3]  Melvin E Andersen,et al.  A dermatotoxicokinetic model of human exposures to jet fuel. , 2006, Toxicological sciences : an official journal of the Society of Toxicology.

[4]  R. Baldwin,et al.  NAPHTHALENE-INDUCED RESPIRATORY TRACT TOXICITY: METABOLIC MECHANISMS OF TOXICITY , 2002, Drug metabolism reviews.

[5]  Peter P. Egeghy,et al.  Dermal Exposure to Jet Fuel JP-8 Significantly Contributes to the Production of Urinary Naphthols in Fuel-Cell Maintenance Workers , 2005, Environmental health perspectives.

[6]  S. Pavanello,et al.  Influence of GSTM1 null and low repair XPC PAT+ on anti-B[a]PDE-DNA adduct in mononuclear white blood cells of subjects low exposed to PAHs through smoking and diet. , 2008, Mutation research.

[7]  Nilesh Gaikwad,et al.  Depurinating naphthalene-DNA adducts in mouse skin related to cancer initiation. , 2009, Free radical biology & medicine.

[8]  V. Riihimäki,et al.  Urinary 1-naphthol and 1-pyrenol as indicators of exposure to coal tar products , 1995, International archives of occupational and environmental health.

[9]  Roger Gibson,et al.  Urinary biomarkers of exposure to jet fuel (JP-8). , 2003, Environmental health perspectives.

[10]  M Pirmohamed,et al.  Characterisation of the toxic metabolite(s) of naphthalene. , 1996, Toxicology.

[11]  S. Nesnow,et al.  32P-postlabelling in studies of polycyclic aromatic hydrocarbon activation. , 1993, IARC scientific publications.

[12]  W. Bigbee,et al.  Biomonitoring of human genotoxicity induced by complex occupational exposures. , 1999, Mutation research.

[13]  T. Sigsgaard,et al.  Exposure of iron foundry workers to polycyclic aromatic hydrocarbons: benzo(a)pyrene-albumin adducts and 1-hydroxypyrene as biomarkers for exposure. , 1994, Occupational and environmental medicine.

[14]  Michelle Salemi,et al.  Identification of proteins adducted by reactive naphthalene metabolites in vitro , 2005, Proteomics.

[15]  L. Hsieh,et al.  The GST T1 and CYP2E1 genotypes are possible factors causing vinyl chloride induced abnormal liver function , 1997, Archives of Toxicology.

[16]  Ho Kim,et al.  Effects of genetic polymorphisms of CYP1A1, CYP2E1, GSTM1, and GSTT1 on the urinary levels of 1-hydroxypyrene and 2-naphthol in aircraft maintenance workers. , 2001, Toxicology letters.

[17]  R. Guy,et al.  The effect of aging on percutaneous absorption in man , 1989, Journal of Pharmacokinetics and Biopharmaceutics.

[18]  N. Rothman,et al.  ANALYTICAL BIOCHEMISTRY , 2003 .

[19]  P. Brennan,et al.  Genetic polymorphisms of MPO, COMT, MnSOD, NQO1, interactions with environmental exposures and bladder cancer risk. , 2004, Carcinogenesis.

[20]  D. McAuliffe,et al.  Penetration of benzene through human skin. , 1985, The Journal of investigative dermatology.

[21]  G N Carlton,et al.  Exposures to jet fuel and benzene during aircraft fuel tank repair in the U.S. Air Force. , 2000, Applied occupational and environmental hygiene.

[22]  F. Furukawa,et al.  Effects of adenosine 5′-monophosphate on epidermal turnover , 2008, Archives of Dermatological Research.

[23]  Tsutomu Shimada,et al.  Xenobiotic-metabolizing enzymes involved in activation and detoxification of carcinogenic polycyclic aromatic hydrocarbons. , 2006, Drug metabolism and pharmacokinetics.

[24]  K. Koskinen,et al.  Glutathione S-transferase and N-acetyltransferase genotypes and asbestos-associated pulmonary disorders. , 1996, Journal of the National Cancer Institute.

[25]  K. Kjaerheim,et al.  Occupational cancer research in the Nordic countries. , 1999, Environmental health perspectives.

[26]  Melvin E Andersen,et al.  Dermal absorption and penetration of jet fuel components in humans. , 2006, Toxicology letters.

[27]  B. K. Park,et al.  The use of a three compartment in vitro model to investigate the role of hepatic drug metabolism in drug-induced blood dyscrasias. , 1993, British journal of clinical pharmacology.

[28]  Obaid Faroon,et al.  Toxicological profile for jet fuels JP-4 and JP-7 , 1995 .

[29]  J. French,et al.  S-arylcysteine-keratin adducts as biomarkers of human dermal exposure to aromatic hydrocarbons. , 2008, Chemical research in toxicology.

[30]  S M Rappaport,et al.  Benzene and naphthalene in air and breath as indicators of exposure to jet fuel , 2003, Occupational and environmental medicine.

[31]  Herman Autrup,et al.  Exposure to genotoxins present in ambient air in Bangkok, Thailand--particle associated polycyclic aromatic hydrocarbons and biomarkers. , 2002, The Science of the total environment.

[32]  L. Hui,et al.  Association of genetic polymorphisms in CYP2E1, MPO, NQO1, GSTM1, and GSTT1 genes with benzene poisoning. , 2002, Environmental health perspectives.

[33]  J. French,et al.  Exposure to naphthalene induces naphthyl-keratin adducts in human epidermis in vitro and in vivo , 2010, Biomarkers : biochemical indicators of exposure, response, and susceptibility to chemicals.

[34]  S. Fustinoni,et al.  Quantification of 13 priority polycyclic aromatic hydrocarbons in human urine by headspace solid-phase microextraction gas chromatography-isotope dilution mass spectrometry. , 2009, Analytica chimica acta.

[35]  Leena A Nylander-French,et al.  Dermal exposure to jet fuel (JP-8) in US Air Force personnel. , 2005, The Annals of occupational hygiene.

[36]  J. Angerer,et al.  Naphthalene—an environmental and occupational toxicant , 2003, International archives of occupational and environmental health.

[37]  L. Kupper,et al.  Investigation of PAH biomarkers in the urine of workers exposed to hot asphalt. , 2009, The Annals of occupational hygiene.

[38]  T. Kawamoto,et al.  A Study for the Proper Application of Urinary Naphthols, New Biomarkers for Airborne Polycyclic Aromatic Hydrocarbons , 1999, Archives of environmental contamination and toxicology.

[39]  P. Shields,et al.  Cytochrome P4502E1 (CYP2E1) genetic polymorphism in a case-control study of gastric cancer and liver disease. , 1995, Pharmacogenetics.

[40]  P. S. Nielsen,et al.  Environmental air pollution and DNA adducts in Copenhagen bus drivers--effect of GSTM1 and NAT2 genotypes on adduct levels. , 1996, Carcinogenesis.

[41]  A. Chokkalingam,et al.  NQO1 polymorphisms and de novo childhood leukemia: a HuGE review and meta-analysis. , 2008, American journal of epidemiology.

[42]  M F Greaves,et al.  A lack of a functional NAD(P)H:quinone oxidoreductase allele is selectively associated with pediatric leukemias that have MLL fusions. United Kingdom Childhood Cancer Study Investigators. , 1999, Cancer research.

[43]  G. Bieniek.  The presence of 1-naphthol in the urine of industrial workers exposed to naphthalene. , 1994, Occupational and environmental medicine.

[44]  F. Watt.  Proliferation and terminal differentiation of human epidermal keratinocytes in culture. , 1988, Biochemical Society transactions.

[45]  S. Rappaport,et al.  Albumin Adducts of Naphthalene Metabolites as Biomarkers of Exposure to Polycyclic Aromatic Hydrocarbons , 2004, Cancer Epidemiology Biomarkers & Prevention.

[46]  L. Kupper,et al.  Comparing urinary biomarkers of airborne and dermal exposure to polycyclic aromatic compounds in asphalt-exposed workers. , 2009, The Annals of occupational hygiene.

[47]  Y. D. Kim,et al.  Effects of occupation, lifestyle and genetic polymorphisms of CYP1A1, CYP2E1, GSTM1 and GSTT1 on urinary 1-hydroxypyrene and 2-naphthol concentrations. , 2001, Carcinogenesis.

[48]  Melvin E. Andersen,et al.  PBTK Modeling Demonstrates Contribution of Dermal and Inhalation Exposure Components to End-Exhaled Breath Concentrations of Naphthalene , 2007, Environmental health perspectives.

[49]  E. Taioli,et al.  Role of GSTT1 deletion in DNA oxidative damage by exposure to polycyclic aromatic hydrocarbons in humans , 2007, International journal of cancer.

[50]  P. S. Nielsen,et al.  Biomarkers for exposure to ambient air pollution--comparison of carcinogen-DNA adduct levels with other exposure markers and markers for oxidative stress. , 1999, Environmental health perspectives.

[51]  S. Loft,et al.  Interaction of smoking, uptake of polycyclic aromatic hydrocarbons, and cytochrome P450IA2 activity among foundry workers. , 1992, British journal of industrial medicine.