Liver Ischemia Modifies the Concentration of Plasmatic Catecholamines after Reperfusion in the Rat

Pulmonary hypertension is one of the most frequent and severe consequences of liver ischemia. The aim of this study is to evaluate the presence of humoral vasoactive mediators, generated during liver ischemia, which could be able to determine the onset of pulmonary hypertension. Thus, we evaluated the plasmatic concentration of catecholamines (adrenaline, noradrenaline, dopamine) during the immediate reperfusion period. Wistar rats were used. Animals (n = 89) were divided into four groups. Group 1 served as control (sham-operated). In group 2 animals underwent 60 min of left hepatic exclusion. In group 3 animals underwent to bilateral adrenectomy. In group 4 animals had both bilateral adrenectomy and liver ischemia. Ischemia in group 2 and 4 was induced by interrupting the vascular supply to the left and median lobes, so avoiding the use of a portal shunt. Blood samples were collected from the suprahepatic inferior caval vein immediately after reperfusion. Strips of the main pulmonary artery were put into an isolated organ bath and tested for the response to noradrenaline, adrenaline and plasma samples. Plasma samples collected after ischemia caused a significantly greater (p < 0.01) contraction of the pulmonary artery compared to controls. Plasma samples collected after adrenectomy caused a weak contraction which was not different from that obtained in the adrenectomy + ischemia group. Plasma concentrations of catecholamines after liver ischemia were significantly increased in the control group (p < 0.01). In adrenectomized rats only the adrenaline level was greatly reduced. However ischemia did not increase plasma catecholamines as it occurred in sham-operated rats.

[1]  M. Reguzzoni,et al.  Quantitative changes in pharmacodynamic parameters of noradrenaline in different rat aorta preparations: influence of endogenous EDRF. , 1998, Journal of autonomic pharmacology.

[2]  L. Toledo-Pereyra,et al.  Tacrolimus (FK506) down-regulates free radical tissue levels, serum cytokines, and neutrophil infiltration after severe liver ischemia. , 1997, Transplantation.

[3]  M. Menger,et al.  Pentoxifylline reduces venular leukocyte adherence ("reflow paradox") but not microvascular "no reflow" in hepatic ischemia/reperfusion. , 1997, The Journal of surgical research.

[4]  G. Otto,et al.  Inhibition of nitric oxide synthesis in ischemia/reperfusion of the rat liver is followed by impairment of hepatic microvascular blood flow. , 1997, Journal of hepatology.

[5]  M. Shiraishi,et al.  Role of exogenous L-arginine in hepatic ischemia-reperfusion injury. , 1997, Journal of Surgical Research.

[6]  P. Boels,et al.  Chronic hypoxia inhibits postnatal maturation of porcine intrapulmonary artery relaxation. , 1997, The American journal of physiology.

[7]  J. Roselló-Catafau,et al.  Liver ischemic preconditioning is mediated by the inhibitory action of nitric oxide on endothelin. , 1996, Biochemical and biophysical research communications.

[8]  S. Steen,et al.  Inhaled nitric oxide reveals and attenuates endothelial dysfunction after lung transplantation. , 1996, The Annals of thoracic surgery.

[9]  B. Gewertz,et al.  Hepatic influence on pulmonary neutrophil sequestration following intestinal ischemia-reperfusion. , 1996, The Journal of surgical research.

[10]  C. Malloy,et al.  Effects of ischemia on intracellular sodium and phosphates in the in vivo rat liver. , 1996, Journal of applied physiology.

[11]  İ. Şahin-Erdemli,et al.  Reactive oxygen species-induced impairment of endothelium-dependent relaxation in rat aortic rings: protection by L-arginine. , 1996, European journal of pharmacology.

[12]  D. Agrawal,et al.  Ischemia-Induced Peripheral Arterial Vasospasm Role of α1- and α2-Adrenoceptors , 1996 .

[13]  M. Maestri,et al.  Evaluation of the reperfusion syndrome after liver ischemia in the rat. , 1996, The Journal of surgical research.

[14]  P. Dartevelle,et al.  L-arginine and pentoxifylline attenuate endothelial dysfunction after lung reperfusion injury in the rabbit. The Paris-Sud University Lung Transplant Group. , 1995, The Annals of thoracic surgery.

[15]  E. Roth,et al.  ARGINASE RELEASE FOLLOWING LIVER REPERFUSION EVIDENCE OF HEMODYNAMIC ACTION OF ARGINASE INFUSIONS , 1995, Transplantation.

[16]  M. Conte,et al.  Pulmonary failure following lower torso ischemia: clinical evidence for a remote effect of reperfusion injury. , 1995, The American surgeon.

[17]  J. Maxa,et al.  The immunosuppressive qualities of calcium channel blockers. , 1993, Transplantation.

[18]  A. Harken,et al.  Intracellular mechanisms of pulmonary vasomotor dysfunction in acute lung injury caused by mesenteric ischemia-reperfusion. , 1993, Surgery.

[19]  A. Hoitsma,et al.  Evidence that simultaneous transplantation of a left kidney and adrenal gland may result in severe hypertension after clamp removal. , 1993, Transplantation.

[20]  D. Martel,et al.  Biochemical appraisal of models for hepatic ischemia-reperfusion injury. , 1992, Circulatory shock.

[21]  J. Pittet,et al.  Protective effect of indomethacin in the development of the postreperfusion syndrome during liver transplantation in pigs. , 1991, Transplantation proceedings.

[22]  L. Toledo-Pereyra,et al.  Histamine and xanthine oxidase in hepatic ischemia-reperfusion injury. , 1990, Transplantation proceedings.

[23]  E. Heidbreder,et al.  Assay of catecholamines and dihydroxyphenylethyleneglycol in human plasma and its application in orthostasis and mental stress. , 1988, Life sciences.

[24]  I. Chaudry,et al.  Hepatic ischemia models for determining the effects of ATP-MgCl2 treatment. , 1986, The Journal of surgical research.

[25]  Van Rossum Jm.  Cumulative dose-response curves. II. Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. , 1963 .

[26]  J. V. van Rossum.  Cumulative dose-response curves. II. Technique for the making of dose-response curves in isolated organs and the evaluation of drug parameters. , 1963, Archives internationales de pharmacodynamie et de therapie.

[27]  R. Furchgott,et al.  Reactions of strips of rabbit aorta to epinephrine, isopropylarterenol, sodium nitrite and other drugs. , 1953, The Journal of pharmacology and experimental therapeutics.