Development of new drug delivery system for implant bone augmentation using a basic fibroblast growth factor-gelatin hydrogel complex.

This study sought to clarify the effectiveness of bFGF-gelatin hydrogel complex on bone regeneration around implants for the development of a new drug delivery system for bone augmentation. Twenty-four titanium implants (03.3 mmX 10 mm) were placed into edentulous areas of the mandibles of four beagle dogs with the upper four screw threads exposed at buccal side. bFGF-gelatin hydrogel complex with 0, 0.1, 1, 10, 100 microg bFGF or autogenous bone (as control) then filled the bone defect site to cover the exposed screw threads. After eight weeks, tissue specimens including implants were evaluated histologically and histomorphometrically. Histological observation showed new bone formation around exposed screw threads in the groups with 1, 10, 100 microg bFGF and autogenous bone-a striking contrast to the groups with contents of 0 and 0.1 microg bFGF. These results thus suggested that bFGF-gelatin hydrogel complex using an optimum amount of bFGF was useful for bone augmentation around implants.

[1]  A. D. Boever,et al.  Guided bone regeneration around non-submerged implants in narrow alveolar ridges: a prospective long-term clinical study. , 2005 .

[2]  S. Shott,et al.  Local application of rhTGF-beta2 enhances peri-implant bone volume and bone-implant contact in a rat model. , 2005, Bone.

[3]  R. Brooks,et al.  The effect of osteogenic growth factors on bone growth into a ceramic filled defect around an implant , 2004, Journal of orthopaedic research : official publication of the Orthopaedic Research Society.

[4]  E. Burger,et al.  Fate of monocortical bone blocks grafted in the human maxilla: a histological and histomorphometric study. , 2003, Clinical oral implants research.

[5]  Y. Tabata,et al.  [Development of new therapeutic method for empyema]. , 2003, Kekkaku : [Tuberculosis].

[6]  I. Kurokawa,et al.  A Therapy-Resistant Chronic Leg Ulcer Treated Successfully with Topical Basic Fibroblast Growth Factor , 2003, The Journal of international medical research.

[7]  S. Santavirta,et al.  Recombinant Human Bone Morphogenetic Protein-2 for Treatment of Open Tibial Fractures: A Prospective, Controlled, Randomized Study of Four Hundred and Fifty Patients , 2002, The Journal of bone and joint surgery. American volume.

[8]  J. Tanaka,et al.  Development of an artificial vertebral body using a novel biomaterial, hydroxyapatite/collagen composite. , 2002, Biomaterials.

[9]  M. Tanihara,et al.  Sustained release of basic fibroblast growth factor and angiogenesis in a novel covalently crosslinked gel of heparin and alginate. , 2001, Journal of biomedical materials research.

[10]  D. Brocard,et al.  Bioabsorbable materials for guided bone regeneration prior to implant placement and 7-year follow-up: report of 14 cases. , 2001, Journal of periodontology.

[11]  M. Maeda,et al.  Controlled local application of basic fibroblast growth factor (FGF-2) accelerates the healing of GBR. An experimental study in beagle dogs. , 2000, Clinical oral implants research.

[12]  Y. Ikada,et al.  Vascularization effect of basic fibroblast growth factor released from gelatin hydrogels with different biodegradabilities. , 1999, Biomaterials.

[13]  M. Chiapasco,et al.  Clinical outcome of autogenous bone blocks or guided bone regeneration with e-PTFE membranes for the reconstruction of narrow edentulous ridges. , 1999, Clinical oral implants research.

[14]  M. Maeda,et al.  Continuous Administration of Basic Fibroblast Growth Factor (FGF-2) Accelerates Bone Induction on Rat Calvaria— An Application of a New Drug Delivery System , 1998, Journal of dental research.

[15]  T. Albrektsson,et al.  Determinants of correct clinical reporting. , 1998, The International journal of prosthodontics.

[16]  Y. Ikada,et al.  Potential efficacy of basic fibroblast growth factor incorporated in biodegradable hydrogels for skull bone regeneration. , 1997, Journal of neurosurgery.

[17]  G. Boering,et al.  Morbidity from iliac crest bone harvesting. , 1996, Journal of oral and maxillofacial surgery : official journal of the American Association of Oral and Maxillofacial Surgeons.

[18]  D. Marion,et al.  Cerebral blood flow and vasoresponsivity within and around cerebral contusions. , 1996, Journal of neurosurgery.

[19]  Y Ikada,et al.  A new model of subretinal neovascularization in the rabbit. , 1995, Investigative ophthalmology & visual science.

[20]  C. Misch,et al.  THE REPAIR OF LOCALIZED SEVERE RIDGE DEFECTS FOR IMPLANT PLACEMENT USING MANDIBULAR BONE GRAFTS , 1995, Implant dentistry.

[21]  E. Ogata,et al.  Stimulation of fracture repair by recombinant human basic fibroblast growth factor in normal and streptozotocin-diabetic rats. , 1994, Endocrinology.

[22]  A. Sugitachi,et al.  [Locoregional therapy in patients with malignant pleural effusion--two different types of "BAC therapy"]. , 1992, Gan to kagaku ryoho. Cancer & chemotherapy.

[23]  S. Lynch,et al.  Effects of the platelet-derived growth factor/insulin-like growth factor-I combination on bone regeneration around titanium dental implants. Results of a pilot study in beagle dogs. , 1991, Journal of periodontology.

[24]  P. Aspenberg,et al.  Dose-dependent stimulation of bone induction by basic fibroblast growth factor in rats. , 1991, Acta orthopaedica Scandinavica.

[25]  P. Branemark,et al.  Marginal tissue reactions at osseointegrated titanium fixtures. (II) A cross-sectional retrospective study. , 1986, International journal of oral and maxillofacial surgery.

[26]  S. Syrjänen,et al.  The effects on extraction wound healing of a new drug combination introduced for use in the prevention of post-extraction complications. A preliminary report. , 1981, The British journal of oral surgery.

[27]  D. Gospodarowicz.  Localisation of a fibroblast growth factor and its effect alone and with hydrocortisone on 3T3 cell growth , 1974, Nature.

[28]  G. Cho.  Healing after posterior mandibular segmental osteotomy in dogs , 2002 .

[29]  A. Tencer,et al.  Effect of controlled release of platelet-derived growth factor from a porous hydroxyapatite implant on bone ingrowth. , 1996, Biomaterials.

[30]  D Buser,et al.  Localized ridge augmentation using guided bone regeneration. 1. Surgical procedure in the maxilla. , 1993, The International journal of periodontics & restorative dentistry.

[31]  W. Becker,et al.  Guided tissue regeneration for implants placed into extraction sockets and for implant dehiscences: surgical techniques and case report. , 1990, The International journal of periodontics & restorative dentistry.