Resistance Screening of Capsicum Germplasm to Pepper Mild Mottle Virus (PMMoV) Pathotypes P1,2 and P1,2,3

Pepper mild mottle virus (PMMoV), a member of the genus Tobamovirus , is one of the most threatening pathogens affecting commercial pepper production, and it is classified into pathotypes P 1,2 and P 1,2,3 . As chemical and physical treatments show limited success in controlling PMMoV, resistant pepper varieties are considered the most effective means of disease control. Two hundred pepper germplasms, including 167 accessions of Capsicum chinense and 5 reference accessions known as resistant L alleles, were assessed using a bioassay to select germplasms resistant to PMMoV. Six accessions, including IT261210, were resistant to both PMMoV pathotypes P 1,2 and P 1,2,3 as they developed necrotic local lesions only on inoculated leaves, whereas no symptoms were observed on the upper leaves. Moreover, RT-PCR results of the upper leaves of these accessions were also negative. Thus, the identified accessions may be a novel source of genetic resistance against present or emerging new pathotypes and may be useful for differentiating L alleles.

[1]  G. Choi,et al.  Resistance Screening to Pepper mild mottle virus Pathotypes in Paprika Cultivars , 2014 .

[2]  G. Choi,et al.  A Pathotype of Pepper mild mottle virus Causing Necrotic Spot Symptoms in Paprika Fruit , 2013 .

[3]  W. Kang,et al.  Development and validation of L allele-specific markers in Capsicum , 2012, Molecular Breeding.

[4]  Jundae Lee,et al.  A Set of Allele-specific Markers Linked to L Locus Resistant to Tobamovirus in Capsicum spp. , 2012 .

[5]  G. Choi,et al.  Cause of the Scion Death in Green Pepper Grafting System by a Tobamovirus , 2011 .

[6]  M. Jahn,et al.  Development of SNP markers linked to the L locus in Capsicum spp. by a comparative genetic analysis , 2009, Molecular Breeding.

[7]  O. Lachman,et al.  A New Pathotype of Pepper mild mottle virus (PMMoV) Overcomes the L4 Resistance Genotype of Pepper Cultivars. , 2008, Plant disease.

[8]  Byung-Dong Kim,et al.  Development of a sequence characteristic amplified region marker linked to the L4 locus conferring broad spectrum resistance to tobamoviruses in pepper plants. , 2008, Molecules and cells.

[9]  J. Ohnishi,et al.  Two Amino Acid Substitutions in the Coat Protein of Pepper mild mottle virus Are Responsible for Overcoming the L(4) Gene-Mediated Resistance in Capsicum spp. , 2007, Phytopathology.

[10]  Dong Hyuk Lee,et al.  Occurrence and Distribution of Viruses Infecting Pepper in Korea , 2005 .

[11]  Shinya Tsuda,et al.  Immunolocalization of Pepper mild mottle virus in Capsicum annuum seeds , 2005, Journal of General Plant Pathology.

[12]  Y. Hikichi,et al.  A new tobamovirus-resistance gene, L1a, of sweet pepper (Capsicum annuum L.) , 2004 .

[13]  T. Sugita,et al.  Development of SCAR Markers Linked to L3 gene in Capsicum , 2004 .

[14]  T. Higashi,et al.  An Immunological System for the Detection of Pepper mild mottle virus in Soil from Green Pepper Fields. , 2004, Plant disease.

[15]  H. Matsunaga,et al.  DNA markers linked to pepper mild mottle virus (PMMoV) resistant locus (L4) in Capsicum , 2003 .

[16]  Maria Helena Pimentel,et al.  Comparative , 1986, American Political Science Review.

[17]  M. C. Heath.  Hypersensitive response-related death , 2000, Plant Molecular Biology.

[18]  E. Radwanski,et al.  Comparative genetics of disease resistance within the solanaceae. , 2000, Genetics.

[19]  M. Serra,et al.  Nucleotide sequence of the genomic RNA of pepper mild mottle virus, a resistance-breaking tobamovirus in pepper. , 1991, The Journal of general virology.

[20]  J. Culver,et al.  Tobacco mosaic virus coat protein: an elicitor of the hypersensitive reaction but not required for the development of mosaic symptoms in Nicotiana sylvestris. , 1989, Virology.

[21]  I. Boukema.  Allelism of genes controlling resistance to TMV in Capsicum L. , 1980, Euphytica.

[22]  H H Flor,et al.  Current Status of the Gene-For-Gene Concept , 1971 .

[23]  Y. Hikichi,et al.  Genetic basis for the hierarchical interaction between Tobamovirus spp. and L resistance gene alleles from different pepper species. , 2011, Molecular plant-microbe interactions : MPMI.

[24]  M. Serra,et al.  The nucleotide sequence of the coat protein genes and 3′ non-coding regions of two resistance-breaking tobamoviruses in pepper shows that they are different viruses , 2005, Archives of Virology.

[25]  J. Culver,et al.  Point mutations in the coat protein gene of tobacco mosaic virus induce hypersensitivity in Nicotiana sylvestris , 1989 .

[26]  D. Altschuh,et al.  Pepper mild mottle virus, a tobamovirus infecting pepper cultivars in Sicily , 1984 .