Bacterial expression, purification and functional characterization of a recombinant chimeric Fab derived from murine mAb BCF2 that neutralizes the venom of the scorpion Centruroides noxius hoffmann.
暂无分享,去创建一个
[1] R. Donovan,et al. Review: Optimizing inducer and culture conditions for expression of foreign proteins under the control of thelac promoter , 1996, Journal of Industrial Microbiology.
[2] L. Possani,et al. Analysis of the immune response induced by a scorpion venom sub-fraction, a pure peptide and a recombinant peptide, against toxin Cn2 of Centruroides noxius Hoffmann. , 2003, Toxicon : official journal of the International Society on Toxinology.
[3] A. Plückthun,et al. Helix-stabilized Fv (hsFv) antibody fragments: substituting the constant domains of a Fab fragment for a heterodimeric coiled-coil domain. , 2001, Journal of molecular biology.
[4] E. Moreau,et al. Construction and functional evaluation of a single-chain antibody fragment that neutralizes toxin AahI from the venom of the scorpion Androctonus australis hector. , 2001, European journal of biochemistry.
[5] Carlos F. Barbas,et al. Phage display: a Laboratory manual , 2014 .
[6] E. Horjales,et al. Antibody BCF2 against scorpion toxin cn2 from Centruroides noxius hoffmann: Primary structure and three‐dimensional model as free fv fragment and complexed with its antigen , 1999, Proteins.
[7] M. Delepierre,et al. Scorpion toxins specific for Na+-channels. , 1999, European journal of biochemistry.
[8] L. Possani,et al. Pharmacokinetics of the toxic fraction of Centruroides limpidus limpidus venom in experimentally envenomed rabbits and effects of immunotherapy with specific F(ab')2. , 1999, Toxicon : official journal of the International Society on Toxinology.
[9] M. Delepierre,et al. Solution structure of toxin 2 from centruroides noxius Hoffmann, a beta-scorpion neurotoxin acting on sodium channels. , 1999, Journal of molecular biology.
[10] M. Mousli,et al. A recombinant single‐chain antibody fragment that neutralizes toxin II from the venom of the scorpion Androctonus australis hector , 1999, FEBS letters.
[11] A. George,et al. Antibody engineering: comparison of bacterial, yeast, insect and mammalian expression systems. , 1998, Journal of immunological methods.
[12] K. Larsson,et al. Identification of Framework Residues in a Secreted Recombinant Antibody Fragment That Control Production Level and Localization inEscherichia coli * , 1997, The Journal of Biological Chemistry.
[13] J. Scherrmann,et al. Pharmacokinetics of Heterologous and Homologous Immunoglobulin G, F(ab‘)2 and Fab after Intravenous Administration in the Rat , 1997, The Journal of pharmacy and pharmacology.
[14] M. Little,et al. High level production of soluble single chain antibodies in small-scale Escherichia coli cultures. , 1997, Journal of immunological methods.
[15] A. Plückthun,et al. High volumetric yields of functional dimeric miniantibodies in Escherichia coli, using an optimized expression vector and high-cell-density fermentation under non-limited growth conditions , 1996, Applied Microbiology and Biotechnology.
[16] J. Lang,et al. Immunoreactivity and pharmacokinetics of horse anti-scorpion venom F(ab')2-scorpion venom interactions. , 1996, Toxicology and applied pharmacology.
[17] L. Possani,et al. Fab fragments of the monoclonal antibody BCF2 are capable of neutralizing the whole soluble venom from the scorpion Centruroides noxius Hoffmann. , 1996, Toxicon : official journal of the International Society on Toxinology.
[18] L. Possani,et al. Structural and functional comparison of toxins from the venom of the scorpions Centruroides infamatus infamatus, Centruroides limpidus limpidus and Centruroides noxius. , 1996, Comparative biochemistry and physiology. Part B, Biochemistry & molecular biology.
[19] R. Sodoyer,et al. CHAPTER 12 – Multicombinatorial Libraries and Combinatorial Infection: Practical Considerations for Vector Design , 1996 .
[20] John McCafferty,et al. Phage display of peptides and proteins : a laboratory manual , 1996 .
[21] M. Ismail. The scorpion envenoming syndrome. , 1995, Toxicon : official journal of the International Society on Toxinology.
[22] A. Plückthun,et al. Effects of overexpressing folding modulators on the in vivo folding of heterologous proteins in Escherichia coli. , 1995, Current opinion in biotechnology.
[23] R. Sodoyer,et al. A new phage display system to construct multicombinatorial libraries of very large antibody repertoires. , 1994, Gene.
[24] J. Peterson,et al. Improved synthesis of Salmonella typhimurium enterotoxin using gene fusion expression systems. , 1994, Gene.
[25] S. Sofer,et al. The management of scorpion envenomation 1993. , 1993, Toxicon : official journal of the International Society on Toxinology.
[26] A. Skerra. Bacterial expression of immunoglobulin fragments. , 1993, Current opinion in immunology.
[27] B. Martin,et al. Amino acid sequence and immunological characterization with monoclonal antibodies of two toxins from the venom of the scorpion Centruroides noxius Hoffmann. , 1992, European journal of biochemistry.
[28] J R Blackwell,et al. A novel strategy for production of a highly expressed recombinant protein in an active form , 1991, FEBS letters.
[29] C. Barbas,et al. Assembly of combinatorial antibody libraries on phage surfaces: the gene III site. , 1991, Proceedings of the National Academy of Sciences of the United States of America.
[30] Johannes Buchner,et al. Protein Aggregation in vitro and in vivo: A Quantitative Model of the Kinetic Competition between Folding and Aggregation , 1991, Bio/Technology.
[31] J. Sambrook,et al. Molecular Cloning: A Laboratory Manual , 2001 .
[32] J. Lee,et al. The stabilization of proteins by sucrose. , 1981, The Journal of biological chemistry.
[33] D. Rodbard,et al. Simultaneous analysis of families of sigmoidal curves: application to bioassay, radioligand assay, and physiological dose-response curves. , 1978, The American journal of physiology.