Self-Assembly Strategy for Reducing Non-Speci�c Adsorption on Substrates and Application for Quantitative Immunoassay

The development of functionalized surfaces with low non-speci�c adsorption is important for biomedical applications. To inhibit non-speci�c adsorption on a substrate, we prepared a novel optical biochip based on a quantum dot �uorescence immunosorbent assay (QD-FLISA), speci�cally by modifying a layer of dense negatively charged �lm (SO 32− ) on the glass substrate surface via self-assembly. Using optimized conditions, we constructed a biochip on functionalized glass substrates to achieve quantitative detection of C-reactive protein (CRP). We subsequently achieved quantitative determination of CRP in the range of 1-1,000 ng/mL, with a limit of detection (LOD) of 1.26 ng/mL or 5.17 ng/mL, using poly(styrene sulfonic acid) sodium salt (PSS) or meso-tetra (4-sulfonatophenyl) porphine dihydrochloride (TSPP) on individually modi�ed glass substrate biochips. The experimental protocol was further optimized and the LOD achieved a sensitivity of 0.69 ng/mL using functionalized TSPP and PSS co-treated glass substrate surfaces for the quantitative detection of CRP.

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