Green- and blue-light-mediated chloroplast migration in the centric diatomPleurosira laevis

SummaryThe existence of two photoreceptors regulating chloroplast orientation was found in the centric diatomPleurosira laevis. Chloroplasts migrate through the transvacuolar cytoplasmic strands according to the light conditions. Weak white light of less than 46 μmol/m2 · s (10 W/m2) induces chloroplast movement to the cortical cytoplasm, which is located next to the plasma membrane (dispersion), while intense white light of more than 92 μmol/m2 · s (20 W/m2) induces chloroplast movement towards the nucleus, which is situated in the center of the cell (assemblage). Chloroplast dispersion was maintained as long as the cells were irradiated with weak white light. Conversely, chloroplast assemblage under intense white light was transient and the chloroplasts were released from assemblage after 15 min. Action spectra determined with the Okazaki Large Spectrograph revealed that the weak white light receptor and the intense white light receptor are characterized by 540 nm and 450 nm optima, respectively.

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