One-step synthesis of redox-active polymer/AU nanocomposites for electrochemical immunoassay of multiplexed tumor markers.

In this work, a simple and sensitive multiplexed immunoassay protocol for simultaneous electrochemical determination of alpha-fetoprotein (AFP) and carcinoembryonic antigen (CEA) was designed using redox-active nanocomposites. As the redox-active species, the poly(o-phenylenediamine) (POPD)/Au nanocomposite and poly(vinyl ferrocene-2-aminothiophenol) (poly(VFc-ATP))/Au nanocomposite were obtained by one-step method which HAuCl4 was used as the oxidant. With Au nanoparticles (AuNPs), the nanocomposites were successful to immobilize labeled anti-CEA and anti-AFP as the immunosensing probes. The proposed electrochemical immunoassay enabled the simultaneous monitoring of AFP and CEA in a wide range of 0.01-100ngmL(-1). The detection limits was 0.006ngmL(-1) for CEA and 0.003ngmL(-1) for AFP (S/N=3). The assay results of serum samples with the proposed method were well consistent with the reference values from standard ELISA method. And the negligible cross-reactivity between the two analytes makes it possesses potential promise in clinical diagnosis.

[1]  Y. Chai,et al.  Hollow platinum decorated Fe3O4 nanoparticles as peroxidase mimetic couple with glucose oxidase for pseudobienzyme electrochemical immunosensor , 2014 .

[2]  Zhanfang Ma,et al.  Polyamidoamine dendrimers-capped carbon dots/Au nanocrystal nanocomposites and its application for electrochemical immunosensor. , 2013, Biosensors & bioelectronics.

[3]  Bongjin Jeong,et al.  Increased electrocatalyzed performance through dendrimer-encapsulated gold nanoparticles and carbon nanotube-assisted multiple bienzymatic labels: highly sensitive electrochemical immunosensor for protein detection. , 2013, Analytical chemistry.

[4]  Zhanfang Ma,et al.  A label-free immunosensor based on graphene nanocomposites for simultaneous multiplexed electrochemical determination of tumor markers. , 2014, Biosensors & bioelectronics.

[5]  R. Levicky,et al.  Polymercaptosiloxane Anchor Films for Robust Immobilization of Biomolecules to Gold Supports , 2003 .

[6]  Changming Mao,et al.  A label-free immunosensor based on modified mesoporous silica for simultaneous determination of tumor markers. , 2011, Biosensors & bioelectronics.

[7]  Zhichuan J. Xu,et al.  Synthesis, Functionalization, and Biomedical Applications of Multifunctional Magnetic Nanoparticles , 2010, Advanced materials.

[8]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[9]  C. Barbero,et al.  Formation of a novel electroactive film by electropolymerization of ortho-aminophenol: Study of its chemical structure and formation mechanism. Electropolymerization of analogous compounds , 1989 .

[10]  Suiping Wang,et al.  A novel electrochemical immunosensor based on ordered Au nano-prickle clusters. , 2008, Biosensors & bioelectronics.

[11]  J. Ho,et al.  Electrochemical immunosensor for cholera toxin using liposomes and poly(3,4-ethylenedioxythiophene)-coated carbon nanotubes. , 2006, Analytical chemistry.

[12]  Bingqian Liu,et al.  Magnetic mesoporous organic-inorganic NiCo2O4 hybrid nanomaterials for electrochemical immunosensors. , 2011, ACS applied materials & interfaces.

[13]  Zhanfang Ma,et al.  Graphene oxide reduced directly by redox probes for multiplexed detection of tumor markers. , 2014, Journal of materials chemistry. B.

[14]  M. Ozkan,et al.  Size control of gold nanoparticles grown on polyaniline nanofibers for bistable memory devices. , 2011, ACS nano.

[15]  John Ballato,et al.  Carbon Nanotube Doped Polyaniline , 2002 .

[16]  Liang Su,et al.  Pt nanoflower/polyaniline composite nanofibers based urea biosensor. , 2011, Biosensors & bioelectronics.

[17]  Hassan Karimi-Maleh,et al.  Simultaneous determination of N-acetylcysteine and acetaminophen by voltammetric method using N-(3,4-dihydroxyphenethyl)-3,5-dinitrobenzamide modified multiwall carbon nanotubes paste electrode , 2011 .

[18]  Feng Yan,et al.  A gold nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate for reagentless electrochemical immunoassay. , 2006, Biomaterials.

[19]  Massimo F. Bertino,et al.  One-pot Synthesis of Polyaniline-metal Nanocomposites , 2005 .

[20]  Yingju Liu,et al.  A dual amplified electrochemical immunosensor for ofloxacin: Polypyrrole film-Au nanocluster as the matrix and multi-enzyme-antibody functionalized gold nanorod as the label , 2013 .

[21]  Ying Zhuo,et al.  Horseradish peroxidase-functionalized Pt hollow nanospheres and multiple redox probes as trace labels for a sensitive simultaneous multianalyte electrochemical immunoassay. , 2010, Chemical communications.

[22]  Zhanfang Ma,et al.  Highly stable electrochemical immunosensor for carcinoembryonic antigen. , 2012, Biosensors & bioelectronics.

[23]  X. Qu,et al.  A graphene functionalized electrochemical aptasensor for selective label-free detection of cancer cells. , 2011, Biomaterials.

[24]  Jing‐Juan Xu,et al.  A branched electrode based electrochemical platform: towards new label-free and reagentless simultaneous detection of two biomarkers. , 2013, Chemical communications.

[25]  Juan Tang,et al.  One-step electrochemical immunosensing for simultaneous detection of two biomarkers using thionine and ferrocene as distinguishable signal tags , 2012, Microchimica Acta.

[26]  Zhanfang Ma,et al.  Au-ionic liquid functionalized reduced graphene oxide immunosensing platform for simultaneous electrochemical detection of multiple analytes. , 2014, Biosensors & bioelectronics.

[27]  J. Do,et al.  Urea biosensor based on PANi(urease)-Nafion/Au composite electrode. , 2004, Biosensors & bioelectronics.

[28]  Wei Yan,et al.  Fabrication of a label-free electrochemical immunosensor of low-density lipoprotein. , 2008, The journal of physical chemistry. B.

[29]  Wei Chen,et al.  Sensitive human interleukin 5 impedimetric sensor based on polypyrrole-pyrrolepropylic acid-gold nanocomposite. , 2008, Analytical chemistry.

[30]  R. V. Gregory,et al.  Kinetic study of the chemical polymerization of aniline in aqueous solutions , 1992 .

[31]  Zhanfang Ma,et al.  Fabrication of an ultrasensitive electrochemical immunosensor for CEA based on conducting long-chain polythiols. , 2013, Biosensors & bioelectronics.

[32]  Hongyuan Chen,et al.  Graphene oxide–thionine–Au nanostructure composites: Preparation and applications in non-enzymatic glucose sensing , 2012 .

[33]  Xiaowei Han,et al.  Simultaneous electrochemical detection of multiple tumor markers using functionalized graphene nanocomposites as non-enzymatic labels , 2014 .

[34]  H. G. Drickamer,et al.  High-pressure spectroscopic studies of ferrocene, nickelocene, and ruthenocene , 1988 .

[35]  Dan Wu,et al.  A novel label-free electrochemical immunosensor based on graphene and thionine nanocomposite , 2010 .

[36]  A. Ramanavičius,et al.  Gold nanoparticle and conducting polymer-polyaniline-based nanocomposites for glucose biosensor design , 2013 .

[37]  Ronen Polsky,et al.  Selective immobilization of DNA and antibody probes on electrode arrays: simultaneous electrochemical detection of DNA and protein on a single platform. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[38]  Jun-Jie Zhu,et al.  Horseradish peroxidase-functionalized gold nanoparticle label for amplified immunoanalysis based on gold nanoparticles/carbon nanotubes hybrids modified biosensor. , 2008, Biosensors & bioelectronics.

[39]  A. Bund,et al.  Formation and electroanalytical performance of polyaniline–palladium nanocomposites obtained via Layer-by-Layer adsorption and electroless metal deposition , 2013 .

[40]  H. Karimi-Maleh,et al.  A novel modified carbon paste electrode based on NiO/CNTs nanocomposite and (9, 10-dihydro-9, 10-ethanoanthracene-11, 12-dicarboximido)-4-ethylbenzene-1, 2-diol as a mediator for simultaneous determination of cysteamine, nicotinamide adenine dinucleotide and folic acid. , 2013, Biosensors & bioelectronics.

[41]  Michael Holzinger,et al.  Aqueous dispersions of SWCNTs using pyrrolic surfactants for the electro-generation of homogeneous nanotube composites. Application to the design of an amperometric biosensor , 2008 .

[42]  S. A. Sebt,et al.  Synthesis and application of FePt/CNTs nanocomposite as a sensor and novel amide ligand as a mediator for simultaneous determination of glutathione, nicotinamide adenine dinucleotide and tryptophan. , 2013, Physical chemistry chemical physics : PCCP.

[43]  C. Pundir,et al.  Construction of a chitosan/polyaniline/graphene oxide nanoparticles/polypyrrole/Au electrode for amperometric determination of urinary/plasma oxalate , 2013 .

[44]  Hassan Karimi-Maleh,et al.  High sensitive voltammetric sensor based on Pt/CNTs nanocomposite modified ionic liquid carbon paste electrode for determination of Sudan I in food samples. , 2013, Food chemistry.

[45]  Yan Liu,et al.  Ionic‐Liquid‐Doped Polyaniline Inverse Opals: Preparation, Characterization, and Application for the Electrochemical Impedance Immunoassay of Hepatitis B Surface Antigen , 2009 .

[46]  Michael S Wilson,et al.  Electrochemical immunosensors for the simultaneous detection of two tumor markers. , 2005, Analytical chemistry.

[47]  Mohammad A. Khalilzadeh,et al.  Sensitive voltammetric determination of epinephrine in the presence of acetaminophen at a novel ionic liquid modified carbon nanotubes paste electrode , 2012 .