Study on Line Imaging Spectroscopy as a Tool for Nitrogen Diagnostics in Precision Farming

Within nitrogen diagnostics hyperspectral imaging has a great potential overcoming many of the problems connected with derived estimates based on canopy reflectance measurements with a low spatial resolution. With this technology it is possible to collect spectral information from thousands of canopy sub areas (pixels) with a resolution of only a few mm. Hence it is now possible to obtain pure leaf estimates of the reflected light provided that the pixels have been pre-classified. However prior to this study it had not been shown that a high spatial resolution combined with a pixel filtration can actually improve nitrogen diagnostic in winter wheat. A hyperspectral line imaging system VTTVIS with a resolution of 1.8 mm (commercialised by Specim Ltd. Finland as Imspector V7) was able to predict the leaf chlorophyll concentration 30% and the leaf nitrogen concentration 38% more accurate than a hyperspectral non-imaging system with a lower resolution of 3.3*10 mm using the spectral range from 438-756 nm in winter wheat at 16 nitrogen levels provided no other plant stress was present. This improved performance using the VTTVIS was due to a two-band classification extracting relevant green leaf pixels using upper and lower thresholds for both R550 and the Normalised Difference between Green and Red (NDGR) [R550R670]/[R550+R670] prior to estimating the mean reflectance spectra used in the prediction models. The optimal limits modelling leaf chlorophyll concentration and leaf nitrogen concentration were found separately. Through a detailed theoretical description and based on ray tracing results of the dispersion Prism-Grating-Prism unit in the equipment, significant image distortion within both the spectral and spatial dimension of the hyperspectral image were proved. Further pattern noise in form of e.g. fixed pattern noise (FPN), lens vignetting, slit width variations, wavelength dependent PhotoResponse NonUniformity (PRNU), fringing patterns, and narrow band quantum efficiency response variation were shown to introduce significant bias in the sensitivity response of the VTTVIS. All known authors using PGP based systems have not described procedures for handling these systematic noise sources. Thus it is likely their published results are confounded with significant system bias. Fast calibration procedures under field conditions using the solar spectrum were developed and tested for spectral calibration with accuracy of ± 0.5 nm and for minimising system pattern noise. However due to limitations of the equipment, it was not possible to develop a comprehensive procedure for completely correcting these errors.

[1]  Luc Van Gool,et al.  Sensor for Weed Detection Based on Spectral Measurements , 1998 .

[2]  Yoshifumi Yasuoka,et al.  SPATIAL ESTIMATION OF BIOCHEMICAL PARAMETERS OF LEAVES WITH HYPERSPECTRAL IMAGER , 2001 .

[3]  T. Borregaard,et al.  Crop–weed Discrimination by Line Imaging Spectroscopy , 2000 .

[4]  Josep Peñuelas,et al.  Evaluating Wheat Nitrogen Status with Canopy Reflectance Indices and Discriminant Analysis , 1995 .

[5]  B. M. Whelan,et al.  VESPER – SPATIAL PREDICTION SOFTWARE FOR PRECISION AGRICULTURE , 2002 .

[6]  W. M. Haynes CRC Handbook of Chemistry and Physics , 1990 .

[7]  Edward J. Milton,et al.  Reference panel anisotropy and diffuse radiation - some implications for field spectroscopy , 2000 .

[8]  Heimo Keraenen,et al.  Advanced prism-grating-prism imaging spectrograph in online industrial applications , 1997, Other Conferences.

[9]  Gregory A. Carter,et al.  Responses of leaf spectral reflectance to plant stress. , 1993 .

[10]  A. Wild,et al.  Russell's Soil Conditions and Plant Growth , 1988 .

[11]  Frede Aakmann Tøgersen,et al.  Statistical Modelling and Deconvolution of Yield Meter Data , 2004 .

[12]  Anders Astrom,et al.  Near-sensor real-time imaging spectroscopy for industrial applications , 1996, Electronic Imaging.

[13]  J. V. Stafford,et al.  Limitations on the spatial resolution of yield mapping for combinable crops , 1997 .

[14]  W. Allen,et al.  Electrooptical remote sensing methods as nondestructive testing and measuring techniques in agriculture. , 1968, Applied optics.

[15]  A. Gitelson,et al.  Quantitative estimation of chlorophyll-a using reflectance spectra : experiments with autumn chestnut and maple leaves , 1994 .

[16]  J. D. De Baerdemaeker,et al.  Site-Specific Relationship Between Grain Quality and Yield , 2004, Precision Agriculture.

[17]  J. Privette,et al.  Impact of Tissue, Canopy, and Landscape Factors on the Hyperspectral Reflectance Variability of Arid Ecosystems , 2000 .

[18]  G. Agati,et al.  New vegetation indices for remote measurement of chlorophylls based on leaf directional reflectance spectra. , 2001, Journal of photochemistry and photobiology. B, Biology.

[19]  Theo Gevers,et al.  Comparison of multispectral images across the Internet , 1999, Electronic Imaging.

[20]  R. W. Leamer,et al.  Reflectance of Wheat Cultivars as Related to Physiological Growth Stages1 , 1980 .

[21]  J. Schepers,et al.  Transmittance and reflectance measurements of corn leaves from plants with different nitrogen and water supply , 1996 .

[22]  Senthold Asseng,et al.  Simulation of grain protein content with APSIM-Nwheat , 2002 .

[23]  Claus Buschmann,et al.  In vivo spectroscopy and internal optics of leaves as basis for remote sensing of vegetation , 1993 .

[24]  Luc Van Gool,et al.  Vision system for weed detection using hyper-spectral imaging, structural field information and unsupervised training sample collection , 1999 .

[25]  Luc Van Gool,et al.  Weed detection based on structural information using an imaging spectrograph , 1998 .

[26]  Gerrit Polder,et al.  Hyperspectral image analysis for measuring ripeness of tomatoes. , 2000 .

[27]  J. V. Stafford,et al.  Grain quality variations within fields of malting barley. , 1999 .

[28]  Esko Herrala,et al.  Imaging spectrometer for process industry applications , 1994, Other Conferences.

[29]  C. Field,et al.  A narrow-waveband spectral index that tracks diurnal changes in photosynthetic efficiency , 1992 .

[30]  T. S. Prasad,et al.  New hyperspectral vegetation characterization parameters , 2001 .

[31]  H.W.G. Booltink,et al.  Site-specific management: balancing production and environmental requirements at farm level. , 1995 .

[32]  Christopher B. Field,et al.  Reflectance indices associated with physiological changes in nitrogen- and water-limited sunflower leaves☆ , 1994 .

[33]  L. Tucker,et al.  Some mathematical notes on three-mode factor analysis , 1966, Psychometrika.

[34]  Theo Gevers,et al.  Color Measurement by Imaging Spectrometry , 2000, Comput. Vis. Image Underst..

[35]  D. Kimes,et al.  Irradiance measurement errors due to the assumption of a Lambertian reference panel , 1982 .

[36]  Luc Van Gool,et al.  Multi-spectral vision system for weed detection , 2001, Pattern Recognit. Lett..

[37]  D. Long,et al.  Method for Precision Nitrogen Management in Spring Wheat: II. Implementation , 2000, Precision Agriculture.

[38]  Josep Peñuelas,et al.  An AOTF-based hyperspectral imaging system for field use in ecophysiological and agricultural applications , 2001 .

[39]  R. H. Dowdy,et al.  Spatial and temporal stability of corn grain yields , 1997 .

[40]  D. Mulla,et al.  A comparison of winter wheat yield and quality under uniform versus spatially variable fertilizer management , 1992 .

[41]  D. Horler,et al.  The red edge of plant leaf reflectance , 1983 .

[42]  H. Keulen,et al.  Performance and application of the APSIM Nwheat model in the Netherlands , 2000 .

[43]  William D. Philpot,et al.  Toward the Discrimination of Manganese, Zinc, Copper, and Iron Deficiency in ‘Bragg’ Soybean Using Spectral Detection Methods , 2000 .

[44]  A. Richardson,et al.  Interaction of Light with a Plant Canopy , 1968 .

[45]  Airborne Imaging Spectrometry in National Forest Inventory , 1996 .

[46]  D. Mulla,et al.  Key processes and properties for site-specific soil and crop management. , 1997 .

[47]  Esko Herrala,et al.  Direct sight imaging spectrograph: a unique add-on component brings spectral imaging to industrial applications , 1998, Electronic Imaging.

[48]  Ray D. Jackson,et al.  Winter wheat vegetation indices calculated from combinations of seven spectral bands , 1985 .

[49]  F. H. Siddoway,et al.  Spring Wheat Yield Estimates from Spectral Reflectance Measurements , 1981, IEEE Transactions on Geoscience and Remote Sensing.

[50]  B. Ma,et al.  Canopy Light Reflectance and Field Greenness to Assess Nitrogen Fertilization and Yield of Maize , 1996 .

[51]  William D. Philpot,et al.  Yellowness index: An application of spectral second derivatives to estimate chlorosis of leaves in stressed vegetation , 1999 .

[52]  J. C. Price How unique are spectral signatures , 1994 .

[53]  C. A. Shull A Spectrophotometric Study of Reflection of Light from Leaf Surfaces , 1929, Botanical Gazette.

[54]  V. K. Choubey,et al.  Spectral Reflectance, Growth and Chlorophyll Relationships for Rice Crop in a Semi-Arid Region of India , 1999 .

[55]  Alfredo Huete,et al.  Effects of standing litter on the biophysical interpretation of plant canopies with spectral indices , 1996 .

[56]  Philip N. Slater,et al.  Discrimination of growth and water stress in wheat by various vegetation indices through clear and turbid atmospheres , 1983 .

[57]  M. S. Moran,et al.  Bidirectional Calibration Results for 11 Spectralon and 16 BaSO4 Reference Reflectance Panels , 1992 .

[58]  M. F. Baumgardner,et al.  Spectra of Normal and Nutrient-Deficient Maize Leaves , 1974 .

[59]  Patrick Wambacq,et al.  Hyperspectral image sensor for weed-selective spraying , 1999, International Symposium on Photonics and Applications.

[60]  N. Broge,et al.  Comparing prediction power and stability of broadband and hyperspectral vegetation indices for estimation of green leaf area index and canopy chlorophyll density , 2001 .

[61]  Darrel L. Williams,et al.  Laser-Induced Fluorescence (LIF) from Plant Foliage , 1987, IEEE Transactions on Geoscience and Remote Sensing.

[62]  Arie N. de Jong,et al.  Band selection from a hyperspectral data-cube for a real-time multispectral 3CCD camera , 2001, SPIE Defense + Commercial Sensing.

[63]  A. Masoni,et al.  Spectral Properties of Leaves Deficient in Iron, Sulfur, Magnesium, and Manganese , 1996 .

[64]  J. Harlan,et al.  Spectral estimation of Green leaf area index of oats , 1985 .

[65]  Gerrit Polder,et al.  Calibration and characterization of spectral imaging systems , 2001, International Symposium on Multispectral Image Processing and Pattern Recognition.

[66]  M. E. Bauer,et al.  Relation of agronomic and multispectral reflectance characteristics of spring wheat canopies , 1983 .

[67]  Kai Makisara,et al.  Design and first test results of the Finnish airborne imaging spectrometer for different applications (AISA) , 1993, Defense, Security, and Sensing.

[68]  R. Jørgensen,et al.  THE RISØ CROPASSESSOR - AN IDEA TO A LOW-COST, ROBUST, SIMPLE, AND MODULAR MEASURING DEVICE BASED ON EXISTING TECHNOLOGY FOR MONITORING THE SPATIAL FIELD CROP VARIATION , 2001 .

[69]  Elizabeth A. Walter-Shea,et al.  An Improved Goniometer System for Calibrating Field Reference-Reflectance Panels* , 1993 .

[70]  John B. Solie,et al.  Effect of growth stage and variety on spectral radiance in winter wheat , 2000 .

[71]  A. G. T. Schut,et al.  Effects of nitrogen stress in grass swards on evolution of ground cover and spectral characteristics of leaf strata , 2002, SPIE Remote Sensing.

[72]  M. S. Moran,et al.  Field calibration of reference reflectance panels , 1987 .

[73]  J. Araus,et al.  Spectral vegetation indices as nondestructive tools for determining durum wheat yield. , 2000 .

[74]  J. Peñuelas,et al.  Assessment of photosynthetic radiation‐use efficiency with spectral reflectance , 1995 .

[75]  Moon S. Kim,et al.  Estimating Corn Leaf Chlorophyll Concentration from Leaf and Canopy Reflectance , 2000 .