Direct Determination of Total Flavones in Powder of Ginkgo biloba Extract by Near - infrared Diffuse Reflection Spectroscopy

Objective:To determine directly the total flavones in powder of Ginkgo biloba extract by combining near - infrared diffuse reflection spectroscopy with the chemometrics method. Methods: After collecting the powder samples with different total flavones concentrations, they were analyzed by HPLC and NIR spectra. Different regression models were used to correlate these data. Furthermore, tests of the best - fitted model with prediction samples were made. Results: MPLS( modified partial least squares) regression model best fitted the data. The multicorrelation coefficients of the prediction samples was 0. 973, the recovery was 96. 15% - 102. 0% ,with RSD of 1. 1%. Conclusions: The new method proposed could be used directly to determine the total flavones in powder of Ginkgo biloba extract with high accuracy. It is fast and convenient. There were potential applications of the method to the areas of instant analysis and on - line control of the powder production.