Non-sterile bio-hydrogen fermentation from food waste in a continuous stirred tank reactor (CSTR): Performance and population analysis

Abstract Bio-hydrogen production from food waste by anaerobic mixed cultures was conducted in a continuous stirred tank reactor (CSTR). The hydraulic retention time (HRT) was optimized in order to maximize hydrogen yield (HY) and hydrogen production rate (HPR). The maximum hydrogen content (38.6%), HPR (379 mL H2/L. d) and HY (261 mL H2/g-VSadded) were achieved at the optimum HRT of 60 h. The major soluble metabolite products were butyric and acetic acids which indicated a butyrate-acetate type fermentation. Operation of CSTR at HRT 60 h could select hydrogen producing bacteria and eliminate lactic acid bacteria and acetogenic bacteria. The microbial community analyzed by polymerase chain reaction-denaturing gradient gel electrophoresis (PCR-DGGE) revealed that the predominant hydrogen producer was Clostridium sp.

[1]  Luis Manuel Rosales-Colunga,et al.  Continuous biohydrogen production using cheese whey: Improving the hydrogen production rate , 2009 .

[2]  N. Ren,et al.  Bio-hydrogen production by mixed culture of photo- and dark-fermentation bacteria , 2010 .

[3]  Chiu-Yue Lin,et al.  Fermentative hydrogen production from starch using natural mixed cultures , 2008 .

[4]  M. Daffé,et al.  The envelope layers of mycobacteria with reference to their pathogenicity. , 1998, Advances in microbial physiology.

[5]  D. L. Hawkes,et al.  Enhancement of hydrogen production from glucose by nitrogen gas sparging. , 2000 .

[6]  W. Mitchell,et al.  Physiology of carbohydrate to solvent conversion by clostridia. , 1998, Advances in microbial physiology.

[7]  Herbert H. P. Fang,et al.  Fermentative Hydrogen Production From Wastewater and Solid Wastes by Mixed Cultures , 2007 .

[8]  G. Antonopoulou,et al.  Effect of substrate concentration on fermentative hydrogen production from sweet sorghum extract , 2011 .

[9]  Jo-Shu Chang,et al.  Operation strategies for biohydrogen production with a high-rate anaerobic granular sludge bed bioreactor , 2004 .

[10]  Jo‐Shu Chang,et al.  Fermentative hydrogen production with Clostridium butyricum CGS5 isolated from anaerobic sewage sludge , 2005 .

[11]  Phase holdups and microbial community in high-rate fermentative hydrogen bioreactors , 2011 .

[12]  D. Monnet,et al.  Transient intestinal carriage after ingestion of antibiotic-resistant Enterococcus faecium from chicken and pork. , 2001, The New England journal of medicine.

[13]  J. Chung,et al.  Bioproduction of hydrogen from food waste by pilot-scale combined hydrogen/methane fermentation , 2010 .

[14]  Joo-Hwa Tay,et al.  Rapid formation of hydrogen‐producing granules in an anaerobic continuous stirred tank reactor induced by acid incubation , 2007, Biotechnology and bioengineering.

[15]  Z. Hu,et al.  Hydrogen Production from Rice Winery Wastewater by Using a Continuously-Stirred Reactor , 2003 .

[16]  C. Chu,et al.  Aspect ratio effect of bioreactor on fermentative hydrogen production with immobilized sludge , 2013 .

[17]  Thomas L. Madden,et al.  Gapped BLAST and PSI-BLAST: a new generation of protein database search programs. , 1997, Nucleic acids research.

[18]  Godfrey Kyazze,et al.  Continuous dark fermentative hydrogen production by mesophilic microflora: principles and progress , 2007 .

[19]  J. Sambrook,et al.  Molecular Cloning: A Laboratory Manual , 2001 .

[20]  C. Deborde,et al.  Interactions between Pyruvate and Lactate Metabolism in Propionibacterium freudenreichii subsp.shermanii: In Vivo 13C Nuclear Magnetic Resonance Studies , 2000, Applied and Environmental Microbiology.

[21]  C Visvanathan,et al.  Anaerobic digestion of municipal solid waste as a treatment prior to landfill. , 2007, Bioresource technology.

[22]  J. Lay,et al.  Biohydrogen production as a function of pH and substrate concentration. , 2001, Environmental science & technology.

[23]  L. Rodrigues,et al.  Kinetic study of fermentative biosurfactant production by Lactobacillus strains , 2006 .

[24]  H Yokoi,et al.  Microbial hydrogen production from sweet potato starch residue. , 2001, Journal of bioscience and bioengineering.

[25]  Seppo Salminen,et al.  Lactic Acid Bacteria , 2004 .

[26]  P. Lawson,et al.  Clostridium frigidicarnis sp. nov., a psychrotolerant bacterium associated with 'blown pack' spoilage of vacuum-packed meats. , 1999, International journal of systematic bacteriology.

[27]  Hang-Sik Shin,et al.  Continuous biohydrogen production in a CSTR using starch as a substrate , 2008 .

[28]  Alissara Reungsang,et al.  Optimization of key factors affecting hydrogen production from food waste by anaerobic mixed culture , 2011 .

[29]  Sheng-Shung Cheng,et al.  Process performance evaluation of intermittent-continuous stirred tank reactor for anaerobic hydrogen fermentation with kitchen waste , 2008 .

[30]  A. E. Greenberg,et al.  Standard methods for the examination of water and wastewater : supplement to the sixteenth edition , 1988 .

[31]  Yu-You Li,et al.  Characterization of microbial community in the two-stage process for hydrogen and methane production from food waste , 2010 .

[32]  Hang-Sik Shin,et al.  Optimization of continuous hydrogen fermentation of food waste as a function of solids retention time independent of hydraulic retention time , 2008 .

[33]  Alissara Reungsang,et al.  Biohydrogen production from sugarcane bagasse hydrolysate by elephant dung: Effects of initial pH and substrate concentration , 2011 .

[34]  M Momirlan,et al.  Current status of hydrogen energy , 2002 .

[35]  D. L. Hawkes,et al.  Sustainable fermentative hydrogen production: challenges for process optimisation , 2002 .

[36]  A. Tawfik,et al.  Two stage anaerobic baffled reactors for bio-hydrogen production from municipal food waste. , 2011, Bioresource technology.

[37]  F. Kargı,et al.  Bio-hydrogen production from waste materials , 2006 .