Fabrication and evaluation of microgrooved polymers as peripheral nerve conduits

Cell alignment plays an important role in the repair of damaged peripheral nerves. The aligned Schwann cells could direct the axonal outgrowth during nerve reconstruction. One way of aligning Schwann cells is to use surface grooves in micrometric dimensions. In this study, microgrooves on chitosan or poly(d,l-lactide) (PLA) were fabricated and the behaviors of Schwann cells and glial cell line C6 on these surfaces were examined. It was found that Schwann cells and C6 cells could be successfully aligned by the microgrooves, and express the genes related to the production of neurotrophic factors. The polymer conduits with microgrooves on the inner surface were implanted in rats to repair the damaged sciatic nerve. The microgrooved conduits were demonstrated to enhance peripheral nerve regeneration as compared to the smooth conduits.

[1]  R. Bunge,et al.  The Schwann cell: Morphology and development , 1995 .

[2]  Yinye Yang,et al.  Performance Modification of Chitosan Membranes Induced by Gamma Irradiation , 2002, Journal of biomaterials applications.

[3]  J. Tanaka,et al.  The chitosan prepared from crab tendons: II. The chitosan/apatite composites and their application to nerve regeneration. , 2003, Biomaterials.

[4]  Matthew J Dalby,et al.  Nucleus alignment and cell signaling in fibroblasts: response to a micro-grooved topography. , 2003, Experimental cell research.

[5]  J. Bishop,et al.  Association of pRas and pRaf-1 in a complex correlates with activation of a signal transduction pathway , 1993, Current Biology.

[6]  J. Salzer.  Mechanisms of adhesion between axons and glial cells , 1995 .

[7]  C. Idé.  Peripheral nerve regeneration , 1996, Neuroscience Research.

[8]  A. Curtis,et al.  The control of cell division by tension or diffusion , 1978, Nature.

[9]  R. Brown,et al.  Adhesion, alignment, and migration of cultured Schwann cells on ultrathin fibronectin fibres. , 1999, Cell motility and the cytoskeleton.

[10]  E. Shooter,et al.  Neurotrophin 3 activation of TrkC induces Schwann cell migration through the c-Jun N-terminal kinase pathway , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[11]  Xiaosong Gu,et al.  The interaction of Schwann cells with chitosan membranes and fibers in vitro. , 2004, Biomaterials.

[12]  Jun Hu,et al.  Nanotopographical guidance of C6 glioma cell alignment and oriented growth. , 2004, Biomaterials.

[13]  Tessa Hadlock,et al.  Manufacture of porous polymer nerve conduits by a novel low-pressure injection molding process. , 2003, Biomaterials.

[14]  G. Paratcha,et al.  The Neural Cell Adhesion Molecule NCAM Is an Alternative Signaling Receptor for GDNF Family Ligands , 2003, Cell.

[15]  S. Green,et al.  Transfection of C6 glioma cells with glia maturation factor upregulates brain-derived neurotrophic factor and nerve growth factor: trophic effects and protection against ethanol toxicity in cerebellar granule cells , 2000, Brain Research.

[16]  G. Gronowicz,et al.  Mineralization and the Expression of Matrix Proteins During In Vivo Bone Development , 1998, Calcified Tissue International.

[17]  J. Fawcett,et al.  The role of Schwann cells in the regeneration of peripheral nerve axons through muscle basal lamina grafts , 1991, Experimental Neurology.

[18]  F. Lyall,et al.  Biomechanics and cells , 1994 .

[19]  A. Papalois,et al.  Beneficial effect of nerve growth factor‐7S on peripheral nerve regeneration through inside‐out vein grafts: An experimental study , 2004, Microsurgery.

[20]  S. Haskill,et al.  Signal transduction from the extracellular matrix , 1993, The Journal of cell biology.

[21]  Martin Rydmark,et al.  Morphology of normal peripheral axons , 1995 .

[22]  P Aebischer,et al.  Syngeneic Schwann cells derived from adult nerves seeded in semipermeable guidance channels enhance peripheral nerve regeneration , 1992, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[23]  C. Wilkinson,et al.  Topographical control of cell behaviour. I. Simple step cues. , 1987, Development.

[24]  A Compston,et al.  Guidance of oligodendrocytes and their progenitors by substratum topography. , 1995, Journal of cell science.

[25]  Surya K Mallapragada,et al.  Directed growth and selective differentiation of neural progenitor cells on micropatterned polymer substrates. , 2006, Biomaterials.

[26]  Y. Gong,et al.  Studies on nerve cell affinity of biodegradable modified chitosan films , 2003, Journal of biomaterials science. Polymer edition.

[27]  S. Hsu,et al.  Oriented Schwann cell growth on microgrooved surfaces , 2005, Biotechnology and bioengineering.

[28]  H. Thoenen,et al.  Enhanced synthesis of brain-derived neurotrophic factor in the lesioned peripheral nerve: different mechanisms are responsible for the regulation of BDNF and NGF mRNA , 1992, The Journal of cell biology.

[29]  Focal adhesion kinase in integrin-mediated signaling. , 1999 .

[30]  C. Heath,et al.  The development of bioartificial nerve grafts for peripheral-nerve regeneration. , 1998, Trends in biotechnology.

[31]  Heinrich Planck,et al.  Rat Schwann cells in bioresorbable nerve guides to promote and accelerate axonal regeneration , 2003, Brain Research.

[32]  K. Vuori.  Integrin Signaling: Tyrosine Phosphorylation Events in Focal Adhesions , 1998, The Journal of Membrane Biology.

[33]  K. Burridge,et al.  Focal adhesions, contractility, and signaling. , 1996, Annual review of cell and developmental biology.

[34]  Joseph P Vacanti,et al.  Biocompatibility analysis of poly(glycerol sebacate) as a nerve guide material. , 2005, Biomaterials.

[35]  S. Mallapragada,et al.  Oriented Schwann cell growth on micropatterned biodegradable polymer substrates. , 2001, Biomaterials.

[36]  S. Madihally,et al.  Porous chitosan scaffolds for tissue engineering. , 1999, Biomaterials.

[37]  S. Waxman.  The Axon : structure, function, and pathophysiology , 1995 .

[38]  D. Puleo,et al.  Mechanisms of fibronectin-mediated attachment of osteoblasts to substrates in vitro. , 1992, Bone and mineral.