Expression of glucose transporter 1 in human endometrial and decidual tissue

Regulation of endometrial glucose transport is important for the decidualization process. Therefore ,we have examined the expression of the glucose transporter protein isoform 1 (GLUT1) in endometrial samples during the menstrual cycle and in decidual tissue by immunohistochemistry ,and GLUT1 mRNA by RNase protection assays. GLUT1 protein was not detected in proliferative endometrial samples ,but was highly expressed in decidual tissue. Placental tissue was highly positive. GLUT1 mRNA could be detected in endometrial samples with an increase in endometria of the late secretory phase (day 25–28) and maximum concentration in the decidua of the 9th–10th week of gestation. Our results show that GLUT1 is differentially expressed in the different phases of the human endometrium with a maximum in the human decidua. Therefore ,GLUT1 may be an important marker for endometrial differentiation.

[1]  I. Simpson,et al.  Cell surface labeling of glucose transporter isoform GLUT4 by bis-mannose photolabel. Correlation with stimulation of glucose transport in rat adipose cells by insulin and phorbol ester. , 1990, The Journal of biological chemistry.

[2]  L. Giudice,et al.  Discovery of new inducible genes in in vitro decidualized human endometrial stromal cells using microarray technology. , 2000, Endocrinology.

[3]  M. Mueckler.  Family of Glucose-Transporter Genes: Implications for Glucose Homeostasis and Diabetes , 1990, Diabetes.

[4]  E. Sheets,et al.  In vitro binding of insulin and epidermal growth factor to human endometrium and endocervix. , 1985, American journal of obstetrics and gynecology.

[5]  H. Kurachi,et al.  Expression and localization of glucose transporter 1 (GLUT1) in the rat oviduct: a possible supplier of glucose to embryo during early embryonic development. , 1995, Biochemical and biophysical research communications.

[6]  M. Caüzac,et al.  Journal of Clinical Endocrinology and Metabolism Printed in U.S.A. Copyright © 1998 by The Endocrine Society Unexpected Expression of Glucose Transporter 4 in , 2022 .

[7]  C. Bondy,et al.  Placental glucose transporter gene expression and metabolism in the rat. , 1993, The Journal of clinical investigation.

[8]  J. Leeton,et al.  Environment of the preimplantation human embryo in vivo: metabolite analysis of oviduct and uterine fluids and metabolism of cumulus cells. , 1996, Fertility and sterility.

[9]  W. Hay.  Placental transport of nutrients to the fetus. , 1994, Hormone research.

[10]  H. Haring,et al.  Tyrosine kinase activity of insulin‐like growth factor I and insulin receptors in human endometrium during the menstrual cycle: Cyclic variation of insulin receptor expression , 1993, Fertility and sterility.

[11]  M. Sakata,et al.  Gestational changes of glucose transporter gene expression in the mouse placenta and decidua , 1996, Journal of endocrinological investigation.

[12]  T. Strowitzki,et al.  Glucose Transporter 4 (GLUT 4) Is Highly Expressed in Mitochondria-rich Oxyphil Cells , 1998 .

[13]  M. Hollenberg,et al.  Basal, oxytocin-, and insulin-stimulated glucose oxidation in human endometrium. , 1987, Canadian journal of physiology and pharmacology.

[14]  L. Giudice,et al.  Growth factors and growth modulators in human uterine endometrium: their potential relevance to reproductive medicine. , 1994, Fertility and sterility.

[15]  M. Caüzac,et al.  Journal of Clinical Endocrinology and Metabolism Printed in U.S.A. Copyright © 1997 by The Endocrine Society The GLUT3 Glucose Transporter Isoform Is Differentially Expressed within Human Placental , 2022 .

[16]  J. Stephenson,et al.  A Comparison of Renal Disease Across Two Continents: The Epidemiology of Diabetes Complications Study and the EURODIAB IDDM Complications Study , 1996, Diabetes Care.

[17]  M. Mueckler.  Implications for Glucose Homeostasis and Diabetes , 1990 .

[18]  P. Pilch,et al.  Regulation of Glucose-Transporter Function , 1990, Diabetes Care.

[19]  L. Giudice,et al.  Steroid and peptide regulation of insulin-like growth factor-binding proteins secreted by human endometrial stromal cells is dependent on stromal differentiation. , 1992, The Journal of clinical endocrinology and metabolism.