Antibacterial activity and cell viability of hyaluronan fiber with silver nanoparticles.

Silver has been used since time immemorial in different chemical form to treat burns, wounds and several different infections caused by pathogenic bacteria, advancement of biological process of nanoparticles synthesis is evolving into a key area of nanotechnology. The current study deals with the green synthesis, characterization, and evaluation of the biological activity and cell viability of hyaluronan fibers with incorporated silver nanoparticles (HA-Ag NPs). Hyaluronan fiber was prepared by the dissolving of sodium hyaluronate (HA) in aqueous alkaline solution to prepare a transparent solution, which was used for the preparation of fibers by a wet-spinning technique. Consequently, hyaluronan fiber was used as capping and stabilizing agent for the preparation of fibers with silver nanoparticles. HA-Ag NPs were confirmed by transmission electron microscopy, dynamic light scattering, UV/VIS spectroscopy, scanning electron microscopy, energy-dispersive X-ray spectroscopy, thermal analysis, nuclear magnetic resonance, Fourier transform infrared spectroscopy, and X-ray photoelectron spectroscopy. HA-Ag NPs showed high antibacterial activity of against Staphylococcus aureus and Escherichia coli. Cell viability tests indicated that hyaluronan, hyaluronan fibers and hyaluronan fibers with silver nanoparticles were non-toxic on the cell growth. Two different particles size of Ag NPs (10, 40 nm) had not any toxicity till the concentration limit. These tests were performed using mouse fibroblast cell line 3T3.

[1]  A. Cowin,et al.  The Effect of a Hyaluronic Acid–Based Nasal Pack on Mucosal Healing in a Sheep Model of Sinusitis , 2005, American journal of rhinology.

[2]  A. S. Aly,et al.  A novel method for the preparation of silver/chitosan-O-methoxy polyethylene glycol core shell nanoparticles , 2012, Journal of Polymers and the Environment.

[3]  Ke Karlovu,et al.  The bactericidal effect of silver nanoparticles , 2010 .

[4]  A. S. Aly,et al.  Antibacterial cotton fabrics treated with core-shell nanoparticles. , 2012, International journal of biological macromolecules.

[5]  Jiawen Ren,et al.  Preparation of Tractable Platinum, Rhodium, and Ruthenium Nanoclusters with Small Particle Size in Organic Media. , 2000 .

[6]  Sun Yan,et al.  Preparation of aspirin and probucol in combination loaded chitosan nanoparticles and in vitro release study , 2009 .

[7]  A. Bauer,et al.  Antibiotic susceptibility testing by a standardized single disk method. , 1966, American journal of clinical pathology.

[8]  Federica Chiellini,et al.  Chitosan—A versatile semi-synthetic polymer in biomedical applications , 2011 .

[9]  Arnab Roy,et al.  Characterization of enhanced antibacterial effects of novel silver nanoparticles , 2007, Nanotechnology.

[10]  K. Tománková,et al.  The targeted antibacterial and antifungal properties of magnetic nanocomposite of iron oxide and silver nanoparticles. , 2011, Biomaterials.

[11]  Beom Soo Kim,et al.  Rapid biological synthesis of silver nanoparticles using plant leaf extracts , 2009, Bioprocess and biosystems engineering.

[12]  Wan Md Zin Wan Yunus,et al.  Silver/poly (lactic acid) nanocomposites: preparation, characterization, and antibacterial activity , 2010, International journal of nanomedicine.

[13]  R. Upadhyay,et al.  Enhanced antibacterial activity of bifunctional Fe3O4-Ag core-shell nanostructures , 2009 .

[14]  V. Velebný,et al.  The comparison of impedance-based method of cell proliferation monitoring with commonly used metabolic-based techniques. , 2009, Neuro endocrinology letters.

[15]  G. Olah,et al.  Synthesis and Applications of Palladium-Coated Poly(vinylpyridine) Nanospheres , 2000 .

[16]  N. Dagalakis,et al.  Design of an artificial skin. Part III. Control of pore structure. , 1980, Journal of biomedical materials research.

[17]  Margaret M. Smith,et al.  Significant synovial pathology in a meniscectomy model of osteoarthritis: modification by intra-articular hyaluronan therapy , 2008, Rheumatology.

[18]  A. Moustakas,et al.  Hyaluronan Fragments Induce Endothelial Cell Differentiation in a CD44- and CXCL1/GRO1-dependent Manner*boxs , 2005, Journal of Biological Chemistry.

[19]  Shuhong Yu,et al.  Large scale photochemical synthesis of M@TiO2 nanocomposites (M = Ag, Pd, Au, Pt) and their optical properties, CO oxidation performance, and antibacterial effect , 2010 .

[20]  A. Hameed,et al.  Antibacterial Characterization of Silver Nanoparticles against E. Coli ATCC-15224 , 2009 .

[21]  S. Purna,et al.  Collagen based dressings--a review. , 2000, Burns.

[22]  R. Savani,et al.  Hyaluronan-mediated angiogenesis in vascular disease: uncovering RHAMM and CD44 receptor signaling pathways. , 2007, Matrix biology : journal of the International Society for Matrix Biology.

[23]  I. Martin,et al.  Differential cartilaginous tissue formation by human synovial membrane, fat pad, meniscus cells and articular chondrocytes. , 2007, Osteoarthritis and cartilage.

[24]  Glenn H Fredrickson,et al.  The science of hyaluronic acid dermal fillers , 2008, Journal of cosmetic and laser therapy : official publication of the European Society for Laser Dermatology.

[25]  R. Murray,et al.  Water-Soluble, Isolable Gold Clusters Protected by Tiopronin and Coenzyme A Monolayers , 1999 .

[26]  P. Krishnamoorthy,et al.  EXTRACTION OF SILVER NANOPARTICLES FROM THE LEAF EXTRACTS OF CLERODENDRUM INERME , 2010 .

[27]  J. Baker,et al.  Antimicrobial mechanism of action of surfactant lipid preparations in enteric Gram‐negative bacilli , 2000, Journal of applied microbiology.

[28]  Younan Xia,et al.  Nucleation and growth mechanisms for Pd-Pt bimetallic nanodendrites and their electrocatalytic properties , 2010 .

[29]  George John,et al.  Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil. , 2008, Nature materials.

[30]  S. Lin,et al.  Design and evaluation of drug-loaded wound dressing having thermoresponsive, adhesive, absorptive and easy peeling properties. , 2001, Biomaterials.

[31]  Luis M. Liz-Marzán,et al.  Reduction and Stabilization of Silver Nanoparticles in Ethanol by Nonionic Surfactants , 1996 .

[32]  F. Cui,et al.  A mechanistic study of the antibacterial effect of silver ions on Escherichia coli and Staphylococcus aureus. , 2000, Journal of biomedical materials research.

[33]  W. Akeson,et al.  High-molecular-weight hyaluronan inhibits macrophage proliferation and cytokine release in the early wound of a preclinical postlaminectomy rat model. , 2006, The spine journal : official journal of the North American Spine Society.

[34]  H. Navsaria,et al.  Hyaluronic acid: the scientific and clinical evidence. , 2007, Journal of plastic, reconstructive & aesthetic surgery : JPRAS.

[35]  Fred C Tenover,et al.  Mechanisms of antimicrobial resistance in bacteria. , 2006, The American journal of medicine.

[36]  P. J. Powers.  Engaged mentors offer inspiration and open doors. , 2006, American Journal of Medicine.

[37]  C. Araújo,et al.  Laser ablated silver nanoparticles with nearly the same size in different carrier media , 2010 .

[38]  I. Hampson,et al.  Angiogenesis induced by degradation products of hyaluronic acid. , 1985, Science.

[39]  Pamila Gupta,et al.  Investigation of Antibacterial Properties of Silver Nanoparticle-loaded Poly ( acrylamide-co-itaconic acid )-Grafted Cotton Fabric , 2008 .

[40]  S. Saxena,et al.  BIOINSPIRED SYNTHESIS OF SILVER NANOPARTICLES , 2009 .

[41]  G. Prestwich,et al.  Composition of Hyaluronan Affects Wound Healing in the Rabbit Maxillary Sinus , 2006, American journal of rhinology.

[42]  J. Holmes,et al.  Steric Stabilization of Nanocrystals in Supercritical CO2 Using Fluorinated Ligands , 2000 .

[43]  Shant Kumar,et al.  Angiogenic oligosaccharides of hyaluronan enhance the production of collagens by endothelial cells. , 1993, Journal of cell science.

[44]  C. Wai,et al.  Synthesizing and Dispersing Silver Nanoparticles in a Water-in-Supercritical Carbon Dioxide Microemulsion. , 1999, Journal of the American Chemical Society.

[45]  Jong Seok Kim.  Antibacterial Activity of Ag+ Ion-Containing Silver Nanoparticles Prepared Using the Alcohol Reduction Method , 2007 .

[46]  L. Rice,et al.  Antimicrobial resistance prevention initiative: Proceedings of an expert panel on resistance - Roundtable discussion , 2006 .

[47]  M. Rubner,et al.  Antibacterial properties of Ag nanoparticle loaded multilayers and formation of magnetically directed antibacterial microparticles. , 2005, Langmuir : the ACS journal of surfaces and colloids.

[48]  Chi-Ming Che,et al.  Proteomic analysis of the mode of antibacterial action of silver nanoparticles. , 2006, Journal of proteome research.

[49]  S. Jeong,et al.  Antibacterial properties of padded PP/PE nonwovens incorporating nano-sized silver colloids , 2005 .

[50]  J. Pujol,et al.  Differential effects of hyaluronan and its fragments on fibroblasts: Relation to wound healing , 2008, Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society.

[51]  Jie Fu,et al.  Completely "green" synthesis and stabilization of metal nanoparticles. , 2003, Journal of the American Chemical Society.

[52]  J F Barrett,et al.  Structure, function, and assembly of cell walls of gram-positive bacteria. , 1983, Annual review of microbiology.

[53]  R. Murray,et al.  THE LOCATION OF THE MUCOPEPTIDE IN SECTIONS OF THE CELL WALL OF ESCHERICHIA COLI AND OTHER GRAM-NEGATIVE BACTERIA. , 1965, Canadian journal of microbiology.

[54]  R. Pasricha,et al.  Controlled interlinking of Au and Ag nanoclusters using 4-aminothiophenol as molecular interconnects. , 2004, Journal of colloid and interface science.

[55]  R. Abdel-Rahman,et al.  Green synthesis of hyaluronan fibers with silver nanoparticles. , 2012, Carbohydrate polymers.

[56]  E. Bandala,et al.  Enhanced Antibacterial Activity of CeO2 Nanoparticles by Surfactants , 2013 .

[57]  P. Hamal,et al.  Antifungal activity of silver nanoparticles against Candida spp. , 2009, Biomaterials.

[58]  M. Slevin,et al.  Angiogenic Oligosaccharides of Hyaluronan Induce Multiple Signaling Pathways Affecting Vascular Endothelial Cell Mitogenic and Wound Healing Responses* , 2002, The Journal of Biological Chemistry.

[59]  Hongwei Ma,et al.  Facile synthesis and application of Ag-chemically converted graphene nanocomposite , 2010 .

[60]  Jongsik Kim.  Reduction of Silver Nitrate in Ethanol by Poly ( N-vinylpyrrolidone ) , 2007 .

[61]  Jie Fu,et al.  A simple and green method for the synthesis of Au, Ag, and Au-Ag alloy nanoparticles , 2006 .

[62]  M. Yacamán,et al.  The bactericidal effect of silver nanoparticles , 2005, Nanotechnology.

[63]  T. Fan,et al.  Angiogenesis in a delayed revascularization model is accelerated by angiogenic oligosaccharides of hyaluronan. , 1995, Laboratory investigation; a journal of technical methods and pathology.

[64]  R. P. Nachane,et al.  A novel one-pot 'green' synthesis of stable silver nanoparticles using soluble starch. , 2006, Carbohydrate research.

[65]  Juan C. Scaiano,et al.  The biocompatibility and antibacterial properties of collagen-stabilized, photochemically prepared silver nanoparticles. , 2012, Biomaterials.