Three small ribooligonucleotides with specific arginine sites.

Arginine-binding RNA motifs are important to protein-RNA interaction and perhaps also for Archean biochemistry. Selection-amplification was used to isolate three RNAs that are eluted by free arginine from an L-arginine affinity column (Kd approximately 0.2-0.4 mM). The binding sites contain specific internal and bulge loops, whose sequences can include arginine coding triplets. Binding is highly specific for arginine, but all three motifs, like the self-splicing group I intron, also bind guanosine 5'-monophosphate. One site is stereoselective, somewhat preferring D-arginine.

[1]  O. Uhlenbeck,et al.  In vitro selection of RNAs that undergo autolytic cleavage with Pb2+. , 1992, Biochemistry.

[2]  Michael Famulok,et al.  Stereospecific recognition of tryptophan agarose by in vitro selected RNA , 1992 .

[3]  J. Szostak,et al.  In vitro selection of RNA molecules that bind specific ligands , 1990, Nature.

[4]  M Yarus,et al.  A specific amino acid binding site composed of RNA. , 1988, Science.

[5]  J. Puglisi,et al.  Conformation of the TAR RNA-arginine complex by NMR spectroscopy. , 1992, Science.

[6]  O. Uhlenbeck,et al.  Synthesis of small RNAs using T7 RNA polymerase. , 1989, Methods in enzymology.

[7]  François Michel,et al.  The guanosine binding site of the Tetrahymena ribozyme , 1989, Nature.

[8]  D. Lazinski,et al.  Sequence-specific recognition of RNA hairpins by bacteriophage antiterminators requires a conserved arginine-rich motif , 1989, Cell.

[9]  I. Majerfeld,et al.  Co-optimization of ribozyme substrate stacking and L-arginine binding. , 1992, Journal of molecular biology.

[10]  M. Zuker.  On finding all suboptimal foldings of an RNA molecule. , 1989, Science.

[11]  B Tidor,et al.  Arginine-mediated RNA recognition: the arginine fork , 1991, Science.

[12]  M. Yarus,et al.  Genetic code origins , 1989, Nature.

[13]  M Yarus,et al.  Specificity of arginine binding by the Tetrahymena intron. , 1989, Biochemistry.

[14]  D. Turner,et al.  Improved free-energy parameters for predictions of RNA duplex stability. , 1986, Proceedings of the National Academy of Sciences of the United States of America.

[15]  L. Gold,et al.  Systematic evolution of ligands by exponential enrichment: RNA ligands to bacteriophage T4 DNA polymerase. , 1990, Science.

[16]  A. Frankel,et al.  Specific binding of arginine to TAR RNA. , 1992, Proceedings of the National Academy of Sciences of the United States of America.

[17]  D M Crothers,et al.  Fragments of the HIV-1 Tat protein specifically bind TAR RNA. , 1990, Science.