PRELIMINARY STUDY ON CONTROLLING BLACK FUNGI DWELLING ON STONE MONUMENTS BY USING A MICROWAVE HEATING SYSTEM

Microcolonial black fungi have their natural ecological niche on rocks and walls of hypogean environments, playing an important role in the deterioration of materials and aesthetical alteration of monumental stones and mortars. Three black fungi (Sarcinomyces sp., Pithomyces sp. and Scolecobasidium sp.) have been isolated from cultural assets of historical interest. These fungal strains have been used to test the microwave heating method as a new control methodology for eradicating the fungal biological growth on cultural stone artifacts. This methodology is based on a 2.45 GHz microwave electromagnetic radiation, generated by a new apparatus with an appropriate applicator. The first results showed the best dose of 65°C for three minutes, for all the investigated fungal strains. This methodology is very promising because is safety for the operator and the environment, and can be easily applied on site. The use of this method to kill biodeteriogens can avoid the application of chemicals formulates potentially dangerous for substrates and environment.

[1]  Yan Wu,et al.  Inactivation of bacteria and fungus aerosols using microwave irradiation , 2010 .

[2]  Arturo Casadevall,et al.  Ionizing Radiation Changes the Electronic Properties of Melanin and Enhances the Growth of Melanized Fungi , 2007, PloS one.

[3]  A. Ignesti,et al.  Microwave Destruction of Woodworms , 1994 .

[4]  L. Giurgiulescu,et al.  Preliminary researches regarding the microwaves influence on the milk microflora. , 2010 .

[5]  B. Eicher,et al.  Effects of microwave and radio frequency electromagnetic fields on lichens. , 1996, Bioelectromagnetics.

[6]  Č. Franěk,et al.  Mobile irradiator and its application in the preservation of the objects of art , 1986 .

[7]  G. Calò,et al.  Analysis of Microwave Thermal Treatment of Antique Books with Metallic Insets , 2007, The Journal of microwave power and electromagnetic energy : a publication of the International Microwave Power Institute.

[8]  Jae Hee Jung,et al.  Treatment of Fungal Bioaerosols by a High-Temperature, Short-Time Process in a Continuous-Flow System , 2009, Applied and Environmental Microbiology.

[9]  W. Krumbein,et al.  Growth control of algae and cyanobacteria on historical monuments by a mobile UV unit (MUVU) , 1980 .

[10]  D. Angelis,et al.  THE EFFECT OF MICROWAVES ON MICROORGANISMS , 2005 .

[11]  K. Whitehead,et al.  Black fungal colonies as units of survival: hyphal mycosporines synthesized by rock-dwelling microcolonial fungi , 2003 .

[12]  K Kwarecki,et al.  Response of Aspergillus nidulans and Physarum polycephalum to microwave irradiation. , 1980, The Journal of microwave power.

[13]  K. Sterflinger,et al.  Molecular taxonomy and biodiversity of rock fungal communities in an urban environment (Vienna, Austria) , 2001, Antonie van Leeuwenhoek.

[14]  Cesáreo Sáiz-Jiménez,et al.  The chemical structure of fungal melanins and their possible contribution to black stains in stone monuments , 1995 .

[15]  Katja Sterflinger,et al.  Dematiaceous fungi as a major agent for biopitting on Mediterranean marbles and limestones , 1997 .

[16]  S. Priori,et al.  A Portable Microwave System for Woodworm Disinfestation of Artistic Painted Boards , 1997 .

[17]  M. Willert-Porada,et al.  Advances in Microwave and Radio Frequency Processing , 2006 .

[18]  S Banik,et al.  Bioeffects of microwave--a brief review. , 2003, Bioresource technology.

[19]  I. L. Brunetti,et al.  Candida albicans inactivation and cell membrane integrity damage by microwave irradiation , 2007, Mycoses.

[20]  A. Nováková,et al.  The microbiology of Lascaux Cave. , 2010, Microbiology.

[21]  L. Breeding,et al.  Microwave disinfection of denture base materials colonized with Candida albicans. , 1999, The Journal of prosthetic dentistry.

[22]  W. E. Krumbein,et al.  Black fungi in marble and limestones — an aesthetical, chemical and physical problem for the conservation of monuments , 1995 .

[23]  G R Vela,et al.  Mechanism of lethal action of 2,450-MHz radiation on microorganisms , 1979, Applied and environmental microbiology.

[24]  K. Sterflinger,et al.  Microcolonial fungi from antique marbles in Perge/Side/Termessos (Antalya/Turkey) , 2007, Antonie van Leeuwenhoek.

[25]  R. Górny,et al.  Viability of fungal and actinomycetal spores after microwave radiation of building materials. , 2007, Annals of agricultural and environmental medicine : AAEM.

[26]  W. Krumbein,et al.  Sarcinomyces petricola, a new microcolonial fungus from marble in the Mediterranean basin , 1997, Antonie van Leeuwenhoek.

[27]  E. Grant,et al.  The role of water in microwave absorption by biological material with particular reference to microwave hazards. , 1979, Physics in medicine and biology.

[28]  C. Urzì On microbes and art: the role of microbial communities in the degradation and protection of cultural heritage. , 1999, Environmental microbiology.

[29]  A. Casadevall,et al.  Ionizing radiation: how fungi cope, adapt, and exploit with the help of melanin. , 2008, Current opinion in microbiology.

[30]  C. Ruibal,et al.  Isolation and characterization of melanized fungi from limestone formations in Mallorca , 2005, Mycological Progress.

[31]  G. D. Hoog,et al.  A new meristematic fungus, Pseudotaeniolina globosa , 2004, Antonie van Leeuwenhoek.

[32]  S. Chavan,et al.  Microwave baking in food industry: a review. , 2010 .

[33]  F. Wiendl,et al.  The Applicability of Gamma Radiation to the Control of Fungi in Naturally Contaminated Paper , 1995 .