Tissue diagnosis of intestinal microsporidiosis using a fluorescent stain with Uvitex 2B.

AIMS--To detect intestinal microsporidiosis in paraffin wax embedded biopsy specimens using a fluorescence technique incorporating optical brighteners. METHODS--Eight HIV infected patients with confirmed intestinal microsporidiosis (six with Enterocytozoon bieneusi, one with Encephalitozoon intestinalis and one with Encephalitozoon cuniculi infection) and 10 without infection were studied. Tissue sections of paraffin wax embedded duodenal biopsy specimens were stained with 1% Uvitex 2B, coded and analysed independently by two investigators. RESULTS--In all eight cases with confirmed intestinal microsporidian infection, spores could be detected easily in tissue sections using the fluorescence technique. Spores or other elements consistent with microsporidiosis were not found in the 10 patients without infection. CONCLUSION--Staining of tissue sections from paraffin wax embedded intestinal biopsy specimens with stains incorporating Uvitex 2B is a rapid and easy technique for the diagnosis of intestinal microsporidiosis.

[1]  V. Diehl,et al.  Immunologically confirmed disseminated, asymptomatic Encephalitozoon cuniculi infection of the gastrointestinal tract in a patient with AIDS. , 1995, Clinical infectious diseases : an official publication of the Infectious Diseases Society of America.

[2]  D. Schwartz,et al.  Human microsporidial infections , 1994, Clinical Microbiology Reviews.

[3]  J. Orenstein,et al.  Light microscopic diagnosis of microsporidiosis in patients with AIDS. , 1994, The American journal of gastroenterology.

[4]  E. Canning,et al.  An improved practical and sensitive technique for the detection of microsporidian spores in stool samples. , 1994, Transactions of the Royal Society of Tropical Medicine and Hygiene.

[5]  J. Orenstein,et al.  Tissue diagnosis of intestinal microsporidiosis using the chromotrope-2R modified trichrome stain. , 1993, Archives of pathology & laboratory medicine.

[6]  P. Reiss,et al.  Diagnosis of intestinal and disseminated microsporidial infections in patients with HIV by a new rapid fluorescence technique. , 1993, Journal of clinical pathology.

[7]  E. Nohýnková,et al.  An extremely rapid method for detection of microsporidia in biopsy materials from AIDS patients. , 1993, Folia parasitologica.

[8]  D. Marriott,et al.  The Warthin-Starry stain in the diagnosis of small intestinal microsporidiosis in HIV-infected patients. , 1993, Folia parasitologica.

[9]  E. Canning,et al.  Staining of microsporidian spores by optical brighteners with remarks on the use of brighteners for the diagnosis of AIDS associated human microsporidioses. , 1993, Folia parasitologica.

[10]  B. Gazzard,et al.  Histological diagnosis of intestinal microsporidiosis in patients with AIDS. , 1991, Journal of clinical pathology.

[11]  B. Gazzard,et al.  Histological diagnosis ofintestinal microsporidiosis inpatients withAIDS , 1991 .

[12]  J. Orenstein,et al.  Intestinal microsporidiosis as a cause of diarrhea in human immunodeficiency virus-infected patients: a report of 20 cases. , 1990, Human pathology.