Serum levels of reproductive hormones and ultrasonographic monitoring of ovarian follicles in female cloned dogs.

This study investigated the reproductive characteristics in 2 cloned female beagle dogs (Clones A and B) during proestrus and early estrus. The pre-ovulatory estradiol peak occurred 2 days before the pre-ovulatory luteinizing hormone (LH) surge, while the follicle stimulating hormone surge started concomitantly with the LH surge in both cloned dogs. Serum progesterone levels increased during proestrus and estrus and its concentration on the day of the LH surge was 3.59 and 2.71 ng/ml in Clones A and B, respectively. Gradual follicular growth was observed by ultrasonography during proestrus. Although the numbers are limited, these cloned female dogs showed normal ranges of reproductive hormone levels and follicular changes during proestrous and early estrous stages of the cycle.

[1]  Min Kyu Kim,et al.  Dogs cloned from fetal fibroblasts by nuclear transfer. , 2009, Animal reproduction science.

[2]  W. Hwang,et al.  Protocol for the recovery of in vivo matured canine oocytes based on once daily measurement of serum progesterone. , 2008, Cloning and stem cells.

[3]  G. Jang,et al.  Cloning endangered gray wolves (Canis lupus) from somatic cells collected postmortem. , 2008, Theriogenology.

[4]  G. Jang,et al.  A cloned toy poodle produced from somatic cells derived from an aged female dog. , 2008, Theriogenology.

[5]  H. Shin,et al.  Reproductive fertility of cloned male cats derived from adult somatic cell nuclear transfer. , 2007, Cloning and stem cells.

[6]  S. Rehm,et al.  Estrous cycle-dependent histology and review of sex steroid receptor expression in dog reproductive tissues and mammary gland and associated hormone levels. , 2007, Birth defects research. Part B, Developmental and reproductive toxicology.

[7]  Min Kyu Kim,et al.  Endangered wolves cloned from adult somatic cells. , 2007, Cloning and stem cells.

[8]  G. Jang,et al.  Birth of viable female dogs produced by somatic cell nuclear transfer. , 2007, Theriogenology.

[9]  H. Kooistra,et al.  Temporal relations between plasma concentrations of luteinizing hormone, follicle-stimulating hormone, estradiol-17beta, progesterone, prolactin, and alpha-melanocyte-stimulating hormone during the follicular, ovulatory, and early luteal phase in the bitch. , 2006, Theriogenology.

[10]  Goo Jang,et al.  Dogs cloned from adult somatic cells , 2005, Nature.

[11]  P. Monget,et al.  Clinical, Hormonal, and Hematologic Characteristics of Bovine Calves Derived from Nuclei from Somatic Cells , 2002, Biology of reproduction.

[12]  J. Verstegen,et al.  Comparisons of estradiol, LH and FSH patterns in pregnant and nonpregnant beagle bitches. , 2002, Theriogenology.

[13]  S. Woods,et al.  Cloned mice have an obese phenotype not transmitted to their offspring , 2002, Nature Medicine.

[14]  J. E. Fortune,et al.  Reproductive Characteristics of Cloned Heifers Derived from Adult Somatic Cells1 , 2002, Biology of reproduction.

[15]  A. Schnieke,et al.  Production of gene-targeted sheep by nuclear transfer from cultured somatic cells , 2000, Nature.

[16]  E. Kawakami,et al.  Plasma LH and progesterone levels before and after ovulation and observation of ovarian follicles by ultrasonographic diagnosis system in dogs. , 2000, The Journal of veterinary medical science.

[17]  J. Verstegen,et al.  ASSESSMENT OF OVARIAN CHANGES AROUND OVULATION IN BITCHES BY ULTRASONOGRAPHY, LAPAROSCOPY AND HORMONAL ASSAYS , 1996 .

[18]  A. Yeager,et al.  Ultrasonographic appearance of the ovary and uterus of the bitch during oestrus, ovulation and early pregnancy. , 1993, Journal of reproduction and fertility. Supplement.

[19]  D. Lüerßen,et al.  Ultrasonographic monitoring of follicular development, ovulation and the early luteal phase in the bitch. , 1993, Journal of reproduction and fertility. Supplement.

[20]  P. Chakraborty,et al.  Ultrasonographic appearance of the ovaries of dogs during the follicular and luteal phases of the estrous cycle. , 1992, American journal of veterinary research.

[21]  P. Concannon,et al.  Changes in LH, progesterone and sexual behavior associated with preovulatory luteinization in the bitch. , 1977, Biology of reproduction.