Altered expression of NFY-C and RORA in colorectal adenocarcinomas.

NFY-C, a subunit of the transcription factor NFY, binds to the promoters of several eukaryotic genes, including cell cycle-related genes. RORA is a steroid hormone receptor implicated in a range of important cellular processes. We evaluated the expression of NFY-C and RORA in colorectal adenocarcinomas and normal colonic tissue. NFY-C expression was elevated in adenocarcinomas. Moreover, NFY-C mRNA levels correlated with time to disease progression, while NFY-C protein expression was significantly higher in metastatic disease. RORA expression was downregulated in CRC adenocarcinomas compared to normal controls and correlated with time to disease progression. The role of NFY-C and RORA in CRC merits further investigation.

[1]  A. S. Lee,et al.  Transduction of calcium stress through interaction of the human transcription factor CBF with the proximal CCAAT regulatory element of the grp78/BiP promoter , 1995, Molecular and cellular biology.

[2]  C. Carlberg,et al.  Thiazolidine Diones, Specific Ligands of the Nuclear Receptor Retinoid Z Receptor/Retinoid Acid Receptor-related Orphan Receptor α with Potent Antiarthritic Activity* , 1996, The Journal of Biological Chemistry.

[3]  C. Vagianos,et al.  Bombesin and neurotensin exert antiproliferative effects on oval cells and augment the regenerative response of the cholestatic rat liver , 2010, Peptides.

[4]  M. Schwab,et al.  Common Fragile Sites and Cancer: Targeted Cloning by Insertional Mutagenesis , 2004, Annals of the New York Academy of Sciences.

[5]  Tim Hui-Ming Huang,et al.  Inference of hierarchical regulatory network of estrogen-dependent breast cancer through ChIP-based data , 2010, BMC Systems Biology.

[6]  R. Mantovani,et al.  NF-YC Complexity Is Generated by Dual Promoters and Alternative Splicing , 2009, The Journal of Biological Chemistry.

[7]  L. Miraglia,et al.  A Functional Genomics Strategy Reveals Rora as a Component of the Mammalian Circadian Clock , 2004, Neuron.

[8]  A. Papavassiliou,et al.  HER-3 in colorectal tumourigenesis: from mRNA levels through protein status to clinicopathologic relationships. , 2007, European journal of cancer.

[9]  Yudi Pawitan,et al.  False discovery rate, sensitivity and sample size for microarray studies , 2005, Bioinform..

[10]  W. Albig,et al.  Subunits of the Heterotrimeric Transcription Factor NF-Y Are Imported into the Nucleus by Distinct Pathways Involving Importin β and Importin 13 , 2005, Molecular and Cellular Biology.

[11]  D. Sinnett,et al.  In vivo footprinting analysis of the Glypican 3 (GPC3) promoter region in neuroblastoma cells. , 2007, Biochimica et biophysica acta.

[12]  S Rozen,et al.  Primer3 on the WWW for general users and for biologist programmers. , 2000, Methods in molecular biology.

[13]  Pengyuan Liu,et al.  Common Human Cancer Genes Discovered by Integrated Gene-Expression Analysis , 2007, PloS one.

[14]  E. Coccia,et al.  Transcriptional regulation of the ferritin heavy-chain gene: the activity of the CCAAT binding factor NF-Y is modulated in heme-treated Friend leukemia cells and during monocyte-to-macrophage differentiation , 1997, Molecular and cellular biology.

[15]  H. Kakeya,et al.  Multiple NF‐Y‐binding CCAAT boxes are essential for transcriptional regulation of the human C7orf24 gene, a novel tumor‐associated gene , 2011, The FEBS journal.

[16]  R. Evans,et al.  Isoform-specific amino-terminal domains dictate DNA-binding properties of ROR alpha, a novel family of orphan hormone nuclear receptors. , 1994, Genes & development.

[17]  T. H. van der Kwast,et al.  Quantitative analysis of the decay of immunoreactivity in stored prostate needle biopsy sections. , 2000, American journal of clinical pathology.

[18]  B. Wolpin,et al.  Systemic treatment of colorectal cancer. , 2008, Gastroenterology.

[19]  M. Athar,et al.  Tocotrienol-Rich Fraction of Palm Oil Activates p53, Modulates Bax/Bcl2 Ratio and Induces Apoptosis Independent of Cell Cycle Association , 2004, Cell cycle.

[20]  J. Mariani,et al.  The “CholesteROR” Protective Pathway in the Vascular System , 2004, Arteriosclerosis, thrombosis, and vascular biology.

[21]  R. Hipskind,et al.  Cell type-dependent control of NF-Y activity by TGF-β , 2006, Oncogene.

[22]  R. Mantovani,et al.  A survey of 178 NF-Y binding CCAAT boxes. , 1998, Nucleic acids research.

[23]  G. Superti-Furga,et al.  The deletion of the distal CCAAT box region of the A gamma-globin gene in black HPFH abolishes the binding of the erythroid specific protein NFE3 and of the CCAAT displacement protein. , 1989, Nucleic acids research.

[24]  A. Moorman,et al.  Assumption-free analysis of quantitative real-time polymerase chain reaction (PCR) data , 2003, Neuroscience Letters.

[25]  M. Bellorini,et al.  Cloning and expression of human NF-YC. , 1997, Gene.

[26]  B. Birren,et al.  Disruption of the nuclear hormone receptor RORα in staggerer mice , 1996, Nature.

[27]  N. Jahroudi,et al.  The NFY transcription factor functions as a repressor and activator of the von Willebrand factor promoter. , 2002, Blood.

[28]  H. Tsuda,et al.  Quantitative immunohistochemical evaluation of HER2/neu expression with HercepTestTM in breast carcinoma by image analysis , 2001, Pathology international.

[29]  S. Andò,et al.  Evidence that bergapten, independently of its photoactivation, enhances p53 gene expression and induces apoptosis in human breast cancer cells. , 2009, Current cancer drug targets.

[30]  T. P. Dooley.  Molecular biology of the human phenol sulfotransferase gene family. , 1998, The Journal of experimental zoology.

[31]  Min Gyu Lee,et al.  Glucose Metabolism in Cancer , 2003, Journal of Biological Chemistry.

[32]  David I. Smith,et al.  Common fragile sites, extremely large genes, neural development and cancer. , 2006, Cancer letters.

[33]  M. Tada,et al.  Right- and left-sided colorectal cancers display distinct expression profiles and the anatomical stratification allows a high accuracy prediction of lymph node metastasis. , 2004, The Journal of surgical research.

[34]  W. Reith,et al.  Regulation of MHC class II genes: lessons from a disease. , 1996, Annual review of immunology.

[35]  A. Antonacopoulou,et al.  POLR2F, ATP6V0A1 and PRNP expression in colorectal cancer: new molecules with prognostic significance? , 2008, Anticancer research.

[36]  Mads Thomassen,et al.  Gene expression meta-analysis identifies metastatic pathways and transcription factors in breast cancer , 2008, BMC Cancer.

[37]  P. Ravazoula,et al.  Potential Role of Bcl-2 and Bax mRNA and Protein Expression in Chronic Hepatitis Type B and C: A Clinicopathologic Study , 2003, Modern Pathology.

[38]  R. Ravazzolo,et al.  Nuclear Factor Y Drives Basal Transcription of the Human TLX3, a Gene Overexpressed in T-Cell Acute Lymphocytic Leukemia , 2006, Molecular Cancer Research.

[39]  D. Polizzi,et al.  Activation of the orphan nuclear receptor RORα induces growth arrest in androgen‐independent DU 145 prostate cancer cells , 2001, The Prostate.

[40]  Michael Q. Zhang,et al.  Integrative bioinformatics analysis of transcriptional regulatory programs in breast cancer cells , 2008, BMC Bioinformatics.

[41]  S. Raguz,et al.  Role of the duplicated CCAAT box region in gamma‐globin gene regulation and hereditary persistence of fetal haemoglobin. , 1996, The EMBO journal.

[42]  W. Kim,et al.  NF-Y–Dependent Cyclin B2 Expression in Colorectal Adenocarcinoma , 2007, Clinical Cancer Research.

[43]  A. Jetten.  Recent advances in the mechanisms of action and physiological functions of the retinoid-related orphan receptors (RORs). , 2004, Current drug targets. Inflammation and allergy.

[44]  R. Kuhn,et al.  RORA, a large common fragile site gene, is involved in cellular stress response , 2006, Oncogene.

[45]  D. Green,et al.  Cytoplasmic functions of the tumour suppressor p53 , 2009, Nature.

[46]  M. Motta,et al.  Role of the orphan nuclear receptor ROR alpha in the control of the metastatic behavior of androgen-independent prostate cancer cells. , 2002, Oncology reports.